Oxirane, 2-methyl-, polymer with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)

EC Number:

610-877-7

Cas Number:

52624-57-4

Molecular formula:

C6 H14 O3 . 3 (C3 H6 O . C2 H4 O)x

IUPAC Name:

Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)

Details on test material:

- Name of test material (as cited in study report): LUPRANOL VP 9053
- Analytical purity techn. purity
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The substance was added to the test water without any pretreatment; subsequently the fish were placed into the aquarium

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Length at study initiation: 7.0 cm (range: 6.0 - 8.5 cm)
- Weight at study initiation: 4.4 g (range: 2.8 - 7.1 g)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions: oil-free aerated and charcoal filtered tap water, flow-through system
- Water temperature: 20-22°C
- Acclimation to test water and test temperature: 3 days

- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oil-free air
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.0
- Water temperature: 20 - 22 °C
- Duration of housing: about 2 month
- Medical treatment: Once with 0.05 mg/L Malachite green chloride and once with 10 mg/L tetracycline hydrochloride
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.03 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mmol/L
Alkalinity: 0.8 mmol/L

Test temperature:

20 °C

pH:

7.0 - 7.5

Dissolved oxygen:

7.9 - 8.6 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 1000, 2150, 4640, 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: aquaria
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.4 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, 96 h

EFFECT PARAMETERS MEASURED: mortality, symptoms after 1, 4, 24, 48, 72, 96 h

Reference substance (positive control):

yes

Remarks:

chloracetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4 640 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities: 10000 mg/L treatment group - tumbling after 24 h
- Mortality of control: none

Results with reference substance (positive control):

- LC50: ca. 31 mg/L (48 h) (determined 10 Oct. 1988)