LUMIPOD

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From March 22, 2022 to April 20, 2022

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

For the preparation of the inoculum a sample of aerobic sludge has been selected by the mixed
treatment plant of urban (about 66%) and industrial (about 34%) liquid sewage situated at San Rocco
Monza (MB), Italia. The plant of treatment is managed by “BRIANZACQUE SRL (Monza)”.

Initial conc.:

32.13 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation:

inorg. C analysis

Details on study design:

ASSAY EXECUTION

For assay execution the following preparations have been done using deionized water,
Boitles not used have been eliminated at the end of the test.

Test sample

The elemental analysis of the test item has been determined by REDOX Srl (see results in attachment
N. 3).
Because of the slight solubility of the test item in water, the sample was prepared by ultrasonic
dispersion according to UNI EN ISO 10634. About 20 g of the test sample was added to 800 mL of
medium. Then the sample was sonicated for 40 minutes at 40°C. Finally, the sample was let settle (1
nour) and the surplus was removed. The sample was analyzed at TOC analyzer and the value
obtained was considered as the initial measured concentration.
The sample has been used with the measured initial concentration of 32.13 mg/L of organic carbon
(TOC).

Reference substance

Considering 1-Octanol as reference substance using its molecular formula, a starting measured
concentration of 20.49 mg/L of organic carbon (TOC) has been used.

Blank

As blank has been used water containing culture Medium added with the inoculum of the muds.

Test sample + Reference substance

Test sample and reference substance have been put together in order to have a final concentration
given by the sum of organic carbon (TOC) of the 2 substances (see attachment N. 2 for results).

Abiotic check

Test item has been put together with 50 mg/L HgCle in order to check a possible abiotic degradation.

Conditions of assay

The inoculum has been added in all botiles (see bottle preparation), closed and incubation continued
under stirring in darkness at 20 + 1 °C for 28 days. Data recording is performed continuously by a
validated informatic monitoring system (Labgquard Evisense).
At least 1 hour before every determination of inorganic carbon (TIC) the reaction in one bottle of blank,
one of reference substance and one of the test item has been stopped with the addition of 6 mL of sodium hydroxide (1 M).
Triplicate bottles have been analyzed to detect inorganic carbon (IC) at each check point, except at the end of the test where five bottles have been analyzed.

Reference substance:

other: 1-Octanol

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

84

Sampling time:

28 d

Check point (days)	% of biodegradation reference substance	% of biodegradation Test item	% of biodegradation reference substance + Test item
0	0	0	0
1	44	3	17
3	73	21	-
13	94	59	-
21	105	85	-
28	90	84	92

Interpretation of results:

readily biodegradable

Conclusions:

On the base of results obtained, interpreted in accordance to OECD 310:2014 and to Annex | to the OECD Guideline for Testing of Chemicals “Revised introduction to the OECD guidelines for testing of chemicals, section 3" (OECD, March 2006). the test item “L-LEUCINE, REACTION PRODUCTS WITH 1,4:3,6-DIANHYDRO-D-GLUCITOL, ISO-PR ALC. AND OCTANOYL CHLORIDE” (UVCB substance) should be considered readily biodegradable in aerobic conditions.

Description of key information

On the base of results obtained, interpreted in accordance to OECD 310:2014 and to Annex | to the OECD Guideline for Testing of Chemicals “Revised introduction to the OECD guidelines for testing of chemicals, section 3" (OECD, March 2006). the test item “L-LEUCINE, REACTION PRODUCTS WITH 1,4:3,6-DIANHYDRO-D-GLUCITOL, ISO-PR ALC. AND OCTANOYL CHLORIDE” (UVCB substance) should be considered readily biodegradable in aerobic conditions.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater