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EC number: 429-790-9 | CAS number: 137281-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 December 2008 to 12 January 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-[2-(2-amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoic acid
- EC Number:
- 429-790-9
- EC Name:
- 4-[2-(2-amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoic acid
- Cas Number:
- 137281-39-1
- Molecular formula:
- Hill formula: C15 H14 N4 O3 CAS formula: C15 H14 N4 O3
- IUPAC Name:
- 4-(2-{2-amino-4-oxo-3H,4H,7H-pyrrolo[2,3-d]pyrimidin-5-yl}ethyl)benzoic acid
- Test material form:
- solid: particulate/powder
- Details on test material:
- Name: Compound 202723
Storage condition of test material: Room temperature in dark
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were removed from one replicate of test solution and control on test day 0, 4(hatch), 11, 18, 28 and 32. Quality control (QC) samples were prepared at each sampling interval and remained with the exposure solution samples throughout the analytical process.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:Test substance weighed (weight corrected for purity) and dissolved into dilution water to prepare a 10 mg/L solution
- Controls: Dilution water only
- No evidence of undissolved material
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow (Pimephales promelas)
- Source: On site cultures, brood stock originally from US EPA (Duluth)
- Age at study initiation: embryos = 24 hours old
- Feeding during test: Beginning on Day 4, live brine shrimp 3x daily, ad libitum
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 32 d
Test conditions
- Hardness:
44 to 56 mg/L CaCO3- Test temperature:
- 24 to 26 ºC (continuous monitoring)
- pH:
- 7.0 to 7.5
- Dissolved oxygen:
- 5.2-9.0 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal 10 mg compound 202723/L
Mean Measured: 9.4 mg compound 202723/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Open glass/silicone aquaria (volume 6.5 L). Embryos were incubated glass jars (volume 150 mL) with Nitex screen bottoms which were gently rocked in the vessels.
- Aeration: None
- Type of flow-through: Metering pump
- Renewal rate of test solution: 250 mL per vessel per cycle, 10.2 aquarium volumes per 24 hours, except Days 12 to 29 when it was 6 aquarium volumes per 24 hours
- No. of organisms per vessel: initiated with 30 embryos/vessel; thinned to 10 larvae/vessel after hatch
- No. of vessels per concentration (replicates): 4 vessels/treatment
- No. of vessels per control (replicates): 4 vessels/control
- Biomass loading rate: 0.029 g/L of flowing test solution; 0.049 g/L on Days 12 to 29
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Aerated well water
- Metals: None detected at toxic concentrations
- Pesticides: None detected at toxic concentrations
- Alkalinity: 20 to 22 mg/L as CaCO3
- Conductivity: 250 to 290 µmhos/cm
- Culture medium is the same as test medium:
- Intervals of water quality measurement: Dissolved oxygen, pH and temperature measured daily; hardness, alkalinity, conductivity measured weekly.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark with 30 min transitions
- Light intensity: 1000 to 1400 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
-Dead and live embryos counted daily until hatching complete
-Behavior, appearance, mortality observed daily post-hatch
-Total length and dry weight measured at 28 days post-hatch
TEST CONCENTRATIONS
- Single concentration of 10 mg compound/L (nominal)
- Justification for using less concentrations than requested by guideline: limit test
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- number hatched
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- weight
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- length
- Details on results:
- There was acceptable hatching (>66%) and larval survival (>70%) in the control. No effects due to exposure to compound 202723 were observed (see
Table 1). - Reported statistics and error estimates:
- There were no decreases in hatching success, survival, length or dry weight in the deazaguanine treatment compared to the control. Therefore, no statistical analysis was conducted. See table 1
Any other information on results incl. tables
Table 1: Mean percent hatching success and percent normal larvae at completion of hatch (test day 4) and survival, total length and dry weight of fathead minnow (Pimephales promelas) larvae at test termination of the early life-stage (28 days post-hatch) exposure.
MeanMeasuredConc.(mg compound202723/L) |
EmbryoHatchingSuccess (SD)(%) |
NormalLiveLarvaeat hatch(SD) |
28DaysPost-Hatch |
||
|
|
|
LarvalSurvival (SD)(%) |
TotalLength(SD) (mm) |
DryWeight (SD)(g) |
Control |
78(8) |
100(0) |
88(19) |
27.5(0.57) |
0.0441(0.0050) |
9.4mg |
83(11) |
100(0) |
90(8) |
27.5(0.34) |
0.0443(0.0030) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No statistically significant effects were found on survival, growth (length and dry weight) among fish exposed to a mean measured concentration of 9.4 mg/L of compound 202723. No additonal testing was conducted on compound 202723 as OECD Guideline 210 recommends that concentrations of the substance higher than 10 mg/l need not be tested.
- Executive summary:
Test substance: Compound 202723
Experimental Design: Flow through exposure, 4 replicates of 30 eggs each for embryo portion (4 days), 4 replicates of 10 larvae for post-hatch portion (28 days), endpoints of hatching success, larval survival, dry weight and length
Test Organism: Fathead Minnow (Pimephales promelas)
Test Conditions: 32 day duration, 24 to 26oC, illumination of 16 hours light, 8 hours dark at 94 to 120 footcandles (1000 to 1400 lux). Well water pH 6.8 to 7.0, Specific conductivity: 230 to 260 umhos/bm, Total hardness as Ca~CO3: 40 to 58 mg/L, Total alkalinity as CaCO3: 18 to 20mg/L
Mean Measured concentration: 9.4mg compound 202723/L
Results: No effects were obserced on hatching, survival, growth (length and dry wt) among fish exposed to compound 202723 at 9.4 mg/l
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