Tris(trimethylsilyl) phosphate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material:
Jiu Jiang Tinci Materials Techonology Co.
N200611031
- Purity, including information on contaminants, isomers, etc.:

99.28%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Refrigeration (+2 to +8ºC)
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storag:
Stable
- Stability in the medium, i.e. sensitivity of the test material to hydrolysis and/or photolysis:
Stable
- Solubility and stability of the test material in the solventehicle and the exposure medium:
Soluble in water.
- Reactivity of the test material with the incubation material used (e.g. plastic ware):

Non-reactive.

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Calcium chloride dihydrate CaCl2.2H2O 11.76 g
Dissolved in, and made up to 1 litre with deionized water.
Magnesium sulphate heptahydrate MgSO4.7H2O 4.93 g
Dissolved in, and made up to 1 litre with deionized water.
Sodium hydrogen carbonate NaHCO3 2.59 g
Dissolved in, and made up to 1 litre with deionized water.
Potassium chloride KCl 0.23 g
Dissolved in, and made up to 1 litre with deionized water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:
Daphnia magna
- Age at study initiation (mean and range, SD):
less than 24 h
- Source:
Eurofins AgroSciences services,
Ecotox GmbH
Aquatic Toxicology,Eutinger Strasse 24
75223 Niefern-Öschelbronn,
Germany
- Feeding during test
no


ACCLIMATION
- Acclimation period:
24h
- Acclimation conditions (same as test or not):
same as test
- Type and amount of food:
unicellular green alga, chlorella vulgaris
- Feeding frequency:
once a day
- Health during acclimation (any mortality observed):
none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18.8-19℃

pH:

7.88-8.09

Dissolved oxygen:

7.8-8.3 O2/L

Nominal and measured concentrations:

6， 12， 24， 48， 96 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
growth chamber
- Aeration:
no
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

23.181 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid?
YES
- Relevant effect levels:
EC50 24h=0.5866 mg/L
EC50 48h=0.2954 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of short-term toxicity to aquatic invertebrates is 23.181 mg/L.

Description of key information

The EC50 of short-term toxicity to aquatic invertebrates is 23.181 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

23.181 mg/L