Hexyl acrylate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Data source

Reference

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VII

Author:

Smyth HF, Carpenter CP, Carrol SW, Urbano CP, Streigel JA, Nycum JS

Year:

1969

Bibliographic source:

Am. Ind. Hyg. Assoc. J. Vol 30, 470-476

Materials and methods

Principles of method if other than guideline:

Eye injury in rabbits is recorded in a 10 grade ordinal series and is based upon the degree of corneal necrosis that results from single instillation of 0.5 ml undiluted test substance into conjunctival sac of 5 rabbits. Read immediately unstained and after fluoroscein at 24 hours, with ten grades recognized

GLP compliance:

no

Remarks:

Study predates GLP

Test material

Test material information

Constituent 1

Reference substance name:

Hexyl acrylate

EC Number:

219-698-5

EC Name:

Hexyl acrylate

Cas Number:

2499-95-8

Molecular formula:

C9H16O2

IUPAC Name:

hexyl prop-2-enoate

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source:
- Age at study initiation:
- Weight at study initiation:
- Housing:
- Diet (e.g. ad libitum):
- Water (e.g. ad libitum):
- Acclimation period:

ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL

Duration of treatment / exposure:

Duration of treatment: 24 h

Number of animals or in vitro replicates:

5 Rabbits

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing
- Time after start of exposure:

SCORING SYSTEM: Grade 1 indicates at most a very small areas of necrosis resulting from 0.5 mL undiluted chemical in the eye. Grade 5 indicates a severe burn from 0.005 mL undiluted and Grade 10 indicates a severe burn from 0.5 mL of a 1% solution in water or propylene glycol.

TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Results

Irritation parameter:

other: Irritation grade based on Carpenter et al. (1946)

Basis:

mean

Time point:

24 h

Score:

1

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Grade 1.0 corresponds to Necrosis on less than 5% of cornea

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

Based on the irritation score of Grade 1.0, which corresponds to Necrosis on less than 5% of cornea, n-hexyl acrylate is considered irritating to the eye.

Executive summary:

N-hexyl Acrylate produced evidence of eye irritation in an in-vivo rabbit assay. This finding supports classification of N-hexyl Acrylate as a Category 2 Eye Irritant under the Regulation (EC) 1272/2008 on classification, labeling and packaging of substances and mixtures (CLP).