Hexyl acrylate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Data source

Reference

Reference Type:

publication

Title:

Range Finding Toxicity Data: List VII

Author:

Smyth HF, Carpenter CP, Weil CS, Pozzani UC, Strigel JA, Nycum JS

Year:

1969

Bibliographic source:

American Industrial Hygiene Association Journal 30:470

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Non-fasted animals were treated with a single dose by gastric intubation and observed during 14 days. This study was conducted as a range-finding test according to the method described by Smyth HF Jr. and Carpenter CP (1948).

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Hexyl acrylate

EC Number:

219-698-5

EC Name:

Hexyl acrylate

Cas Number:

2499-95-8

Molecular formula:

C9H16O2

IUPAC Name:

hexyl prop-2-enoate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
-Source: Mellon Institute
-Age at study initiation: 4-5 weeks
-Weight at study initiation: 90-120 g
-Diet: Rockland Rodent diet
Water: ad-libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Details on oral exposure: undiluted substance

Doses:

Logarithmic differing by a factor of 2 (no further detail)

No. of animals per sex per dose:

5 Males

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed daily for mortality; frequency of weighing not specified.
- Necropsy of survivors performed: no

Statistics:

LD 50 calculated by method of Thompson (Bacteri Rev 11: 115 June 1947 Use of moving averages and interpolation to estimate median effective dose) and tables of Weil (Biometrics 8: 249 Sept 1952: Tables for convenient calculation median-effective dose (LD50 or ED50) and instructions in their use). The LD50 is given with ± 1.96 standard deviations.

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

22 932 mg/kg bw

Based on:

test mat.

95% CL:

> 18.9 - <= 35.8

Remarks on result:

other: original data: 26.0 ml/kg LD50 (mg/kg)= LD50 (ml/kg) x density x 1000 = 26.0 x 0.882 x 1000 = 22932.0 mg/kg

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 for n-hexyl acrylate following oral intubation was established at ca. 22392 mg/kg bw (26.0 mL/kg) for males. This finding does not warrant classification of n-hexyl acrylate as an acute oral toxicant under the Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP).

Executive summary:

In the key study (Smyth 1969), male rats were treated orally with single doses of n-hexyl acrylate and observed during 14 days. This study was conducted as a range-finding test according to the method described by Smyth HF Jr. and Carpenter CP (1948). The LD50 for n-hexyl acrylate following oral intubation was established at ca. 22392 mg/kg bw (26.0 mL/kg) for males. This finding does not warrant classification of n-hexyl acrylate as an acute oral toxicant under the Regulation (EC) 1272/2008 on classification, labeling, and packaging of substances and mixtures (CLP).