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EC number: 248-383-5 | CAS number: 27277-00-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, available as unpublished report, minor restrictions in design and/or reporting but otherwise adequate for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The hydrolysis of the test substance was investigated in sterile deionised glass-distilled water at concentrations of 5 and 50 ppm, in the dark at pH 5, 7, and 9 at 25 °C.
- GLP compliance:
- no
- Radiolabelling:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Sub-samples (15mL) were withdrawn from all flasks at the time of application. Further samples were withdrawn from one flask of each type at 1, 3, 7, 14, and 26 days after application. These subsamples were stored at -15°C until required.
- Details on test conditions:
- STOCK SOLUTION:
A stock solution was prepared containing 3.65 µg/µL of the C14-radioactive labeled test substance.
TEST SOLUTIONS:
pH 7 and 9 solution were made from E.I.L. buffer powders. A pH 5 solution was made with a citrate buffer. Aliquots of these solutions (100mL) were transferred to conical flasks and they were sterilized in a Griffin and George autoclave.
After cooling to room temperature, aliquots of a test substance stock solution were added to the flask to make the solution. Duplicates of each solution were used. For the approximately 5 ppm solutions, 137 µL of the radiochemical stock solution was added to 100 mL in each flask. For the approximately 50 ppm solutions, 1274 µL of the radiochemical stock solution plus 100 µL of unlabelled stock solution (containing 96.7 mg of the unlabelled substance in 2.5 mL methanol) was added to 100 mL in each each flask. The flasks were stoppered, sealed with ‘parafilm’ and swirled at 25 °C in the dark.
DESCRIPTION OF SOLUTIONS USED FOR PH HYDROLYSIS STUDY:
- 1A & 1B: pH 5 solution containing 5 ppm test substance
- 2A & 2B: pH 5 solution containing 50 ppm test substance
- 3A & 3B: pH 7 solution containing 5 ppm test substance
- 4A & 4B: pH 9 solution containing 5 ppm test substance
- 5A & 5B: pH 9 solution containing 50 ppm test substance
- 6A & 6B: pH 9 solution containing 5 ppm test substance + 10000 ppm of “Gramoxone U.K.” - Duration:
- 26 d
- pH:
- 9
- Temp.:
- 25
- Initial conc. measured:
- 50 other: ppm
- Duration:
- 26 d
- pH:
- 9
- Temp.:
- 25
- Initial conc. measured:
- 5 other: ppm
- Duration:
- 26 d
- pH:
- 7
- Temp.:
- 25
- Initial conc. measured:
- 5 other: ppm
- Duration:
- 26 d
- pH:
- 5
- Temp.:
- 25
- Initial conc. measured:
- 50 other: ppm
- Duration:
- 26 d
- pH:
- 5
- Temp.:
- 25
- Initial conc. measured:
- 5 other: ppm
- Positive controls:
- no
- Negative controls:
- no
- Transformation products:
- no
- Remarks on result:
- other: see "Details on results"
- Details on results:
- The calculated quantity of radioactivity present in the approximately 5 ppm solution was 2.34E+05 dpm/mL; in the 50 ppm solution the value was 4.68E+05 dpm/mL. Measurement of radioactivity during the experiment showed that there was no loss of radioactivity during a ca. 4 week period.
The autoradiograms of chromagrams showed that there was no significant degradation in any of the solutions 2A, 3A, 5A, and 6B after 7 and 26 days, and the radioactivity was counted as described previously.
Approximately 90% of the radioactivity applied to the plate was recovered and, of this approximately 95% was due to the C14-radioactive labeled test substance. These figures were similar for the 7 and 26 day chromatograms.
The results show that the test substance is stable to hydrolysis at room temperature between pH 5 and 9. This range includes all likely natural water pH’s and so purely chemical breakdown is unlikely under natural conditions. - Validity criteria fulfilled:
- not applicable
- Conclusions:
- The results show that the test substance is stable to hydrolysis at room temperature between pH 5 and 9. This range includes all likely natural water pH’s and so purely chemical breakdown is unlikely under natural conditions.
- Executive summary:
The hydrolysis of the test substance was investigated in sterile deionised glassdistilled water at concentations of 5 and 50 ppm, in the dark at pH 5, 7, and 9 at 25 °C. The calculated quantity of radioactivity present in the approximately 5 ppm solution was 2.34 E5 dpm/mL; in the 50 ppm solution the value was 4.68 E5 dpm/mL. Measurement of radioactivity during the experiment showed that there was no loss of radioactivity during a 4 week period. The autoradiograms of chromagrams showed that there was no significant degradation in any of the solutions after 7 and 26 days. Approximately 90% of the radioactivity applied to the plate was recovered and, of this approximately 95% was due to the C14-radioactive labeled test substance. These figures were similar for the 7 and 26 day chromatograms. The results show that the test substance is stable to hydrolysis at room temperature between pH 5 and 9. This range includes all likely natural water pH’s and so purely chemical breakdown is unlikely under natural conditions.
Reference
The radioactivity present in the pH Hydrolysis studies expressed as a percentage of the calculated applied activity:
Solution |
pH |
Description |
% of calculated activity at time after its addition (days) |
||||
0 |
3 |
7 |
14 |
26 |
|||
1A |
5 |
5 ppm test substance |
104 |
105 |
- |
104 |
- |
1B |
105 |
- |
109 |
- |
110 |
||
2A |
5 |
50 ppm test substance |
105 |
- |
104 |
- |
106 |
2B |
106 |
105 |
- |
107 |
- |
||
3A |
7 |
5 ppm test substance |
102 |
- |
100 |
- |
101 |
3B |
145* |
145.0 |
- |
145 |
- |
||
4A |
9 |
5 ppm test substance |
101 |
102 |
- |
103 |
- |
4B |
105 |
- |
104 |
- |
104 |
||
5A |
9 |
50 ppm test substance |
104 |
- |
101 |
- |
106 |
5B |
104 |
104 |
- |
105 |
- |
||
6A |
9 |
5 ppm test substance plus 10,000 ppm ‘Gramoxone’ |
105.0 |
103 |
- |
106 |
- |
6B |
104 |
- |
102 |
- |
102 |
* erroneous application.
Description of key information
The substance is considered to be hydrolytically stable.
Key value for chemical safety assessment
Additional information
The hydrolysis of the test substance was investigated in sterile deionised glass-distilled water at concentrations of 5 and 50 ppm, in the dark at pH 5, 7, and 9 at 25 °C. The calculated quantity of radioactivity present in the approximately 5 ppm solution was 2.34 E5 dpm/mL; in the 50 ppm solution the value was 4.68 E5 dpm/mL. Measurement of radioactivity during the experiment showed that there was no loss of radioactivity during a 4 week period. The autoradiograms of chromatograms showed that there was no significant degradation in any of the solutions after 7 and 26 days. Approximately 90% of the radioactivity applied to the plate was recovered and, of this approximately 95% was due to the C14-radioactive labelled test substance. These figures were similar for the 7 and 26 day chromatograms. The results show that the test substance is stable to hydrolysis at room temperature between pH 5 and 9. This range includes all likely natural water pH’s and so purely chemical breakdown is unlikely under natural conditions.
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