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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline
Details on test material:
White powder
Specific details on test material used for the study:
Batch 562371/016 - purity : 100%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Variety : StrausStrain : Berlin
Sex : female
Age: between 0 and 24 hours
Origin : Umweltbundesamt Berlin
Arrival of strain : Sept. 27th 2007

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hours

Test conditions

Test temperature:
19.9 - 20.8°C
pH:
7.5 to 7.7
Dissolved oxygen:
8.8mg/L
Nominal and measured concentrations:
Nominal : 1/ 10/ 100 mg/L
Details on test conditions:
Before the study, there is a selection of daphnia. Replciates : Four vessels, each containing 20 mL test solution and five daphnia. Control : Four vessels, each containing 20 mL dilution water and five daphnia. Test vessels glass beakers, nominal volume 50 mL, tall shapeTemperature 20 +/- 2°C
Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.3 mg/L, lying within the demanded range of 0.6 - 1.7 mg/L.
Reported statistics and error estimates:
All validity critera were met; For the estimation of the EC50 of the reference item, the fit showed sufficient statistical correspondence of the data with the dose-responsa equation

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In accordance the guideline OECD 202, the following results could be determined for 1574 ACIDE :
48h and 24h NOEC = 100 mg/L
24h and 48h EC50i > 100 mg/L
Executive summary:

The experiment was performed using three concentrations ranging from 1 to 100 mg/L. Twenty daphnia were exposed to the test ite for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

The concentrations showed no toxicity. None of the animals were immobilised in the control.

The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.3 mg/L, lying within the demanded range of 0.6 - 1.7 mg/L.

The following results could be determined for the 1574 ACIDE :

24 and 48h NOEC = 100mg/L

24 and 48h ECi50 > 100 mg/L

= 1 - 10 mg/L