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EC number: 227-645-2 | CAS number: 5921-65-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 29.11.2012 - 05.12.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Measurement of Samples:
Samples incubation vials were cooled to room temperature and then recapped with HPLC autosampler caps. Measurement was started immediately. - Buffers:
- 1) Buffer-Solution pH = 4:
16.0 mL CH3COOH (2 M), 8 mL CH3COONa, ad 200 mL demineralised water.
2) Buffer-Solution pH = 7:
1.7416 g KH2PO4, 100 mL demineralised water, 2.98 mL NaOH (2 M), ad 200 mL demineralised water.
3) Buffer-Solution pH = 9:
0.6186 g H3BO3, 0.7459 g KCl, 100 mL demineralised water, 2.16 mL NaOH (2 M), ad 200 mL demineralised water. - Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: strerile test vials were used
- Sterilisation method: sterile filtration over 0.2 µm nylon filters
TEST MEDIUM
- Volume used/treatment: a solution of the test item was prepared by dissolving 100 mg Acetoguanamine with 100 mL of water by means of an ultrasonic bath (resulting concentration 1000 mg/L). This stock solution was mixed 1:1 with the respective buffer solution, giving final concentrations of 500 mg/L test item. - Duration:
- 120 h
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 500 mg/L
- Duration:
- 120 h
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 500 mg/L
- Duration:
- 120 h
- pH:
- 9.01
- Temp.:
- 50 °C
- Initial conc. measured:
- 500 mg/L
- Number of replicates:
- 4 per pH (3 for incubation, 1 for measurements at t = 0 h)
2 blanks per pH (1 for incubation, 1 for measurements at t = 0 h) - Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- Preliminary test -TIER 1
No signs of hydrolysis were observed in the preliminary study (tier 1) at pH 4, pH 7 and pH 9. Therefore, the test item can be considered as hydrolytically stable at these pH values. Due to the hydrolytical stability of the test item Acetoguanamine, the performance of Tier 2 is not necessary. - Transformation products:
- no
- Details on hydrolysis and appearance of transformation product(s):
- not applicable
- pH:
- 9
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- > 120 h-1
- Remarks on result:
- hydrolytically stable based on preliminary test
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- > 120 h-1
- Remarks on result:
- hydrolytically stable based on preliminary test
- pH:
- 4
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- > 120 h-1
- Remarks on result:
- hydrolytically stable based on preliminary test
- Validity criteria fulfilled:
- not specified
- Conclusions:
- No signs of hydrolysis were observed in the preliminary study (tier 1) at pH 4, pH 7 and pH 9. Therefore, Acetoguanamine can be considered as hydrolytically stable at these pH values. Due to the hydrolytical stability of the test item Acetoguanamine, the performance of Tier 2 is not necessary.
- Executive summary:
In a study according to OECD Guideline 111 the pH-dependent hydrolysis of Acetoguanamine in water was determined.
A stock solution containing 1000 mg/L of the test item in demineralised water was mixed with buffer solutions (pH values: 4; 7; and 9) in a 1:1 ratio. The resulting solutions were sterilized by filtration over a 0.2 µm Nylon filter, purged with Argon and stored at 50 °C for a period of five days. Samples were analysed at the beginning and after five days. The analysis of the samples (performed with HPLC/UV) showed no significant changes in the concentration of the test item within five days. Therefore, Acetoguanamine can be considered as hydrolytically stable at these pH values.
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Duration:
- 5 d
- Temp.:
- 50 °C
- Initial conc. measured:
- ca. 100 mg/L
- Number of replicates:
- 2
- Positive controls:
- not specified
- Negative controls:
- not specified
- Statistical methods:
- no data
- Preliminary study:
- For the testing of the hydrolysis of 6-phenyl-1,3,5-triazine-2,4-diyldiamine a preliminary study according to OECD guideline 111 has been conducted. With view to the screening criteria for hydrolysis laid down in the guideline and the results of the tier 1 study the test item can be considered as hydrolytically stable (DT50 > 365 d). Thus, no higher tier testing on the hydrolysis was conducted.
- Transformation products:
- no
- pH:
- 4
- Temp.:
- 50 °C
- DT50:
- > 5 d
- pH:
- 7
- Temp.:
- 50 °C
- DT50:
- > 5 d
- pH:
- 9
- Temp.:
- 50 °C
- DT50:
- > 5 d
- Validity criteria fulfilled:
- yes
- Remarks:
- validity criteria for the tier 1 assessment of the hydrolysis has been fulfilled
- Conclusions:
- The test item is considered as hydrolytically stable (DT50 (25 °C) > 365 d).
- Executive summary:
Two replicates of a 100 mg/L sterile aqueous buffer solution of the test item have been examined at 50 °C in a preliminary test according to OECD guideline 111. As no relevant degradation had been observed after five days the substance is considered as hydrolytically stable (DT50 (25°C) > 365 days) in line with the OECD guideline.
- Endpoint:
- hydrolysis
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
- Justification for type of information:
- JUSTIFICATION FOR DATA WAIVING
Refer to attached Document - Reason / purpose for cross-reference:
- data waiving: supporting information
- Reason / purpose for cross-reference:
- data waiving: supporting information
Referenceopen allclose all
For all measured values applies that sm is the value given by the calibration function.
The measured concentrations and the change in tier 1 are presented in the following table:
Table 1: Measured Concentrations Tier 1.
Standards |
|||||
Hours |
Nominal Conc. Srin mg/L |
Peak area in mAU * min |
Measured Conc. Smin mg/L |
Sr/Sm |
Mean Sr/Sm |
0 |
50 |
21.1536 |
51.18 |
0.9769 |
0.9754 |
21.1529 |
|||||
0 |
100 |
42.3896 |
103.06 |
0.9703 |
|
42.4022 |
|||||
0 |
500 |
209.2998 |
510.68 |
0.9791 |
|
209.3283 |
|||||
120 |
100 |
41.7261 |
100.56 |
1.0044 |
1.0044 |
41.0168 |
|||||
pH 4.00 |
|||||
Hours |
Nominal Conc. Srin mg/L |
Peak area in mAU * min |
Measured Conc. Smin mg/L |
Residue in % |
Change in % |
0 |
500 |
206.9591 |
492.29 |
|
|
206.4307 |
|||||
207.203 |
|||||
120 |
500 |
200.2984 |
489.60 |
99.45 |
- 0.55 |
202.3735 |
|||||
202.7249 |
|||||
pH 7.00 |
|||||
Hours |
Nominal Conc. Srin mg/L |
Peak area in mAU * min |
Measured Conc. Smin mg/L |
Residue in % |
Change in % |
0 |
500 |
207.177 |
493.04 |
|
|
207.2308 |
|||||
207.1316 |
|||||
120* |
500 |
(203.3968) |
(496.233) |
|
|
(106.3102) |
(259.1417) |
||||
(52.3535) |
(127.3762) |
||||
120 |
500 |
205.7433 |
491.51 |
99.69 |
- 0.31 |
206.5435 |
|||||
206.8165 |
|||||
pH 9.01 |
|||||
Hours |
Nominal Conc. Srin mg/L |
Peak area in mAU * min |
Measured Conc. Smin mg/L |
Residue in % |
Change in % |
0 |
500 |
206.8145 |
493.63 |
|
|
207.489 |
|||||
207.9805 |
|||||
120 |
500 |
203.6204 |
495.19 |
100.32 |
+ 0.32 |
204.9094 |
|||||
203.7695 |
*The value in brackets were produced by faulty inspections and therefore considered invalid. The samples were reinjected from a different autosampler location.
Assesment:
After five days (120 hours), the concentrations of the test item were close to 100 % at all three pH values. The test item can be considered hydrolytically stable.
During determination of concentrations after 120 h, erroneous injections of the autosampler occured in vials 2 and 3 of pH 7 sample, resulting in apparent deviation of -50% of the initial concentration in vial 2 and -0.75 % in vial 3. These data are shown in brackets in table 1. After moving the vials to another position in the autosampler tray, the samples were injected again. In the re-injection, all three vials yielded nearly identical values (RSD 0.27 %). Only the values from the second injection were used for calculations.
Description of key information
Hydrolysis of Caprinoguanamine is not expected under environmental conditions based on its structure, supported by data from structural closely related substances.
Key value for chemical safety assessment
Additional information
Degradation by hydrolysis of Caprinoguanamine is not expected under environmental conditions based on its structure, supported by data from structural closely related substances.
Measured data on hydrolysis are available from OECD 111 guideline studies with Acetoguanamine and Benzoguanamine. In both studies measurements at pH values of 4, 7 and 9 show no significant changes in the concentration of the test item within five days. Both, Acetoguanamine and Benzoguanamine were proven to be hydrolytically stable.
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