1-Naphthaleneethanamine, 7-methoxy-, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.9 (Biodegradation: Zahn-Wellens Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

604-144-0

EC Number:

604-144-0

Cas Number:

139525-77-2

Molecular formula:

C13H15NO.HCl

IUPAC Name:

604-144-0

Test material form:

solid: particulate/powder

Details on test material:

White powder

Specific details on test material used for the study:

Batch : 839411/006Purity : 100.9%Expiry date : 10 Feb 2009Storage : room temperature (20 +/- 5°C), dry

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

The sludge was taken from the activation basin of th ESN sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdord (date of collection : 8 nov 2007)The sludge was washed with tap water twice, then filtrated through a cloth and resuspended in test medium. It was then aerated. The dry matter was determined with 3960 mg suspended solids/L.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 200 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Before sampling for DOC analysis, the pH was measured and adjusted, if necessary, evaporated water was replenished. From each flask; sample of 5 ml were taken on days 0.125 - 2 - 4 - 8 12 - 15 - 20 - 27 and 28. The samples were membrane filtrated and the DOC was measured.

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

All criteria were met : difference within replicates : 0,2% (target value <20%)Degradation of reference item : 7 days (target value < 14 days)Degradation in the toxicity control on day 14 : 42% (target value > 25%)

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 2

Sampling time:

28 d

Details on results:

As nearly no elimination or no degration occurend, no lag phase and no degradatio phase could be determined

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

According the guideline 302B, Mephtamin HCL is not inherently biodegradable.

Executive summary:

Mephtamin HCL was tested using a concentration of nominally 200 mg organic carbon/L in test medium following OECD 302B.

Aniline was chosen as reference item. Activated sludge from a sewage treating plant was used as inoculum (concentration 600 mg dry matter/L). The test was left running for 28 days.

To measure the degradation of test and Mephtamin HCL, the content of dissolved organic carbon (DOC) in the test vessels was measured nine times during the test.

All validity criteria were met. Degradation of the reference item was 98% after 8 days and of the Mephtamin at the end of the test is 2%

Therefore Mephtamin HCL is not inherently biodegradable.