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Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
January - March 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
yes
Remarks:
only one concentration was used
GLP compliance:
yes (incl. QA statement)
Radiolabelling:
yes
Remarks:
C-14
Vehicle:
no
Details on preparation of test solutions, spiked fish food or sediment:
C-14 labelled estradiol was dissolved in water
Test organisms (species):
Lepomis macrochirus
Route of exposure:
aqueous
Justification for method:
aqueous exposure method used for following reason:
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
>= 21 d
Total depuration duration:
>= 7 d
Test temperature:
23.3-24.7
pH:
7.1-8.3
Dissolved oxygen:
6.0-8.2
Nominal and measured concentrations:
The fish were exposed to 136.1 ng 14C-estradiol/l. (mean measured concentration, based on radioactivity). Nominal concentration was 276 ng/L
Reference substance (positive control):
no
Details on estimation of bioconcentration:
The BCFss is calculated as the ratio of the mean values of the 14C concentration in fish and in water determined during steady state.
BCFss
The BCFss is calculated directly from the measurements and additionally based on lipid normilized value.
Lipid content:
ca. 7.6 %
Time point:
end of exposure
Type:
BCF
Value:
<= 49.1 dimensionless
Basis:
whole body w.w.
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:2 ng/l
Key result
Conc. / dose:
136.1 ng/L
Temp.:
>= 23.3 - <= 24.7 °C
pH:
8.2
Type:
BCF
Value:
108.8 L/kg
Basis:
whole body w.w.
Time of plateau:
10 d
Calculation basis:
steady state
Key result
Conc. / dose:
136.1 ng/L
Temp.:
>= 23.3 - <= 24.7 °C
pH:
8.2
Type:
BCF
Value:
85.9 L/kg
Basis:
normalised lipid fraction
Time of plateau:
10 d
Calculation basis:
steady state
Key result
Elimination:
yes
Parameter:
DT50
Depuration time (DT):
3 d
Remarks on result:
other:
Remarks:
empirical

No substance related behaviour or mortality was observed.

Validity criteria fulfilled:
no
Remarks:
The test concentration varied by more than +/-20%
Conclusions:
BCF (steady state) is 108.8, or 85.9 (lipid normalized), which is lower than the bioconcentration criterion for PBT substances (2000)
Executive summary:

The fish were exposed to 14C labeled estradiol and additionally to the dilution water (tap water) for a period of 21 days. Two replicate tanks for the test concentration and one tanks for the tap water control were used. After the accumulation phase, the fish were kept a futher 7 days for depuration. 40 fish were used for the test solution and 20 for the control group. The concentration of the test substance in the fish and in the water was determined through both phases of the test. The 14C concentration in the fish was analyzed by liquid scintillation after combustion of aliquots of the fish in samples taken on day 4, 6, 10, 14, 21, 24, and 26. The 14C concentration in the water was analyzed by liquid scintillation in samples taken on day 4, 6, 10, 14, 21, 24, 26.

It could be established that the BCF was 108.8, and 85.9, if based on lipid normalized (6%) value.

Description of key information

The fish were exposed to 14C labeled estradiol and additionally to the dilution water (tap water) for a period of 21 days. Two replicate tanks for the test concentration and one tank for the tap water control were used. After the accumulation phase, the fish were held a futher 7 days for depuration. 40 fish were used for the test solution and 20 for the control group.
The concentration of the test substance in the fish and in the water was determined through both phases of the test. The 14C concentration in the fish was analyzed by liquid scintillation after combustion of aliquots of the fish in samples taken on day 4, 6, 10, 14, 21, 24, and 26. The 14C concentration in the water was analyzed by liquid scintillation in samples taken on day 4, 6, 10, 14, 21, 24, and 26.



Results: The BCF was 108.8 or 85.9 if lipid normalized (6%).

Key value for chemical safety assessment

BCF (aquatic species):
108.8 L/kg ww

Additional information

The study was performed under GLP (report A52549).