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EC number: 200-023-8 | CAS number: 50-28-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- January - March 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Deviations:
- yes
- Remarks:
- only one concentration was used
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- yes
- Remarks:
- C-14
- Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- C-14 labelled estradiol was dissolved in water
- Test organisms (species):
- Lepomis macrochirus
- Route of exposure:
- aqueous
- Justification for method:
- aqueous exposure method used for following reason:
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- >= 21 d
- Total depuration duration:
- >= 7 d
- Test temperature:
- 23.3-24.7
- pH:
- 7.1-8.3
- Dissolved oxygen:
- 6.0-8.2
- Nominal and measured concentrations:
- The fish were exposed to 136.1 ng 14C-estradiol/l. (mean measured concentration, based on radioactivity). Nominal concentration was 276 ng/L
- Reference substance (positive control):
- no
- Details on estimation of bioconcentration:
- The BCFss is calculated as the ratio of the mean values of the 14C concentration in fish and in water determined during steady state.
BCFss
The BCFss is calculated directly from the measurements and additionally based on lipid normilized value. - Lipid content:
- ca. 7.6 %
- Time point:
- end of exposure
- Type:
- BCF
- Value:
- <= 49.1 dimensionless
- Basis:
- whole body w.w.
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:2 ng/l
- Key result
- Conc. / dose:
- 136.1 ng/L
- Temp.:
- >= 23.3 - <= 24.7 °C
- pH:
- 8.2
- Type:
- BCF
- Value:
- 108.8 L/kg
- Basis:
- whole body w.w.
- Time of plateau:
- 10 d
- Calculation basis:
- steady state
- Key result
- Conc. / dose:
- 136.1 ng/L
- Temp.:
- >= 23.3 - <= 24.7 °C
- pH:
- 8.2
- Type:
- BCF
- Value:
- 85.9 L/kg
- Basis:
- normalised lipid fraction
- Time of plateau:
- 10 d
- Calculation basis:
- steady state
- Key result
- Elimination:
- yes
- Parameter:
- DT50
- Depuration time (DT):
- 3 d
- Remarks on result:
- other:
- Remarks:
- empirical
- Validity criteria fulfilled:
- no
- Remarks:
- The test concentration varied by more than +/-20%
- Conclusions:
- BCF (steady state) is 108.8, or 85.9 (lipid normalized), which is lower than the bioconcentration criterion for PBT substances (2000)
- Executive summary:
The fish were exposed to 14C labeled estradiol and additionally to the dilution water (tap water) for a period of 21 days. Two replicate tanks for the test concentration and one tanks for the tap water control were used. After the accumulation phase, the fish were kept a futher 7 days for depuration. 40 fish were used for the test solution and 20 for the control group. The concentration of the test substance in the fish and in the water was determined through both phases of the test. The 14C concentration in the fish was analyzed by liquid scintillation after combustion of aliquots of the fish in samples taken on day 4, 6, 10, 14, 21, 24, and 26. The 14C concentration in the water was analyzed by liquid scintillation in samples taken on day 4, 6, 10, 14, 21, 24, 26.
It could be established that the BCF was 108.8, and 85.9, if based on lipid normalized (6%) value.
Reference
No substance related behaviour or mortality was observed.
Description of key information
The fish were exposed to 14C labeled estradiol and additionally to the dilution water (tap water) for a period of 21 days. Two replicate tanks for the test concentration and one tank for the tap water control were used. After the accumulation phase, the fish were held a futher 7 days for depuration. 40 fish were used for the test solution and 20 for the control group.
The concentration of the test substance in the fish and in the water was determined through both phases of the test. The 14C concentration in the fish was analyzed by liquid scintillation after combustion of aliquots of the fish in samples taken on day 4, 6, 10, 14, 21, 24, and 26. The 14C concentration in the water was analyzed by liquid scintillation in samples taken on day 4, 6, 10, 14, 21, 24, and 26.
Results: The BCF was 108.8 or 85.9 if lipid normalized (6%).
Key value for chemical safety assessment
- BCF (aquatic species):
- 108.8 L/kg ww
Additional information
The study was performed under GLP (report A52549).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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