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Ecotoxicological information

Endpoint summary

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Additional information

Short-term toxicity to fish

The acute toxicity of the substance (benzylamine)trifluoroborone was investigated according to EU Method C.1 in compliance with GLP. Brachydanio rerio (Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 22.1 °C - 22.4 °C (control: 21.9 °C - 22.4 °C) and a pH range of 7.4 - 7.6 (control: 7.5 - 7.7) and a substance concentration of 100 mg/L. Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As result a LC 100 of > 100 mg/L is reported.

Short-term toxicity to aquatic invertebrates

(benzylamine)trifluoroboron was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 in compliance with GLP. Daphnia magna STRAUS (clone 5) was used as test organism and where exposed for 48 hours to the test substance. A control was included, potassium dichromate was used as a reference substance to test the sensitivitiy of the test system. It was a static freshwater test at 20 ± 1°C with a light-dark cycle (16 hr of light and 8 hr of darkness), all validity criteria for the study as given within the guideline were met. The screening-test was performed using nominal 1 mg/L, 10 mg/L 100 mg/L and 1000 mg/L of the test item, the main test was prepared using nominal 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L and 160 mg/L. The sample concentrations were measured as TOC. As a conclusion of the analytical part of the study the reported EC values should refer to measured concentrations. As a result an EC50 of 66.5 mg/L is reported.

Toxicity to aquatic algae

The toxicity of „(benzylamine)trifluoroboron“ towards algae was tested according to OECDGuideline No. 201 in compliance with GLP. Desmodesmus subspicatus CHODAT was used as test organism and were exposed during 72 hours to the test substance. A control was included, no reference substance is mentioned. It was a freshwater test at 24.3-25.3 °C at constant illumination, all validity criteria within the guideline were met. The screening test was performed using nominal 1 mg/L, 10 mg/L, 100 mg/L and 1000 mg/L of the test item, the main test was prepared using nominal 2.56 mg/L, 6.4 mg/L, 16 mg/L, 40 mg/L and 100 mg/L. The sample concentrations were measured as TOC. As a conclusion of the analytical part of the study the reported EC values should refer to measured concentrations. As results an effect based on growth rate (ErC50(72h)) of 290.930 mg/L and a NOEC(r) < 0.210 mg/L are reported.

Toxicity to microorganisms

The acute toxicity to microorganisms of (benzylamine)trifluoroborn was tested according to EU Method C.11. in compliance with GLP. A mixed population of aquatic microorganisms (activated sludge), taken from a laboratory scale sewage treatment plant unit which runs predominantly with domestic sewage (South Wupper area water authority) was used as inoculum. An incubation period of 30 min was chosen with aeration. Two controls without test item were included in the test design. The sensitivity of the activated sludge was checked using 3.5 -Dichlorophenol as reference compound. The physico-chemical oxygen consumption control was carried out, since some substances may consume oxygen by chemical reactivity. Direct weighings were prepared to give the different test item concentrations, which were 1000, 1800, 3200, 5600 and 10000 mg/L. 3.5 -Dichlorophenol was used in the concentrations of 2.5, 5, 10, 20 and 40 mg/L. 10000 mg/L test item was included in the physico-chemical oxygen consumption control. No analytical methods were applied. Analysis of the respiration rates at the end of the test gave the following result: EC50 = 5850 mg/L. The EC50 value of the reference compound was measured to be 15 mg/L, thus being in the validity range of 5 - 30 mg/L.