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EC number: 203-109-3 | CAS number: 103-41-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04.10.1999 - 08.10.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Feb. 1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Guideline B.5 "Acute Toxicity (Eye Irritation)"
- Version / remarks:
- Jan. 1997
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Benzyl cinnamate
- EC Number:
- 203-109-3
- EC Name:
- Benzyl cinnamate
- Cas Number:
- 103-41-3
- Molecular formula:
- C16H14O2
- IUPAC Name:
- benzyl 3-phenylacrylate
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight: 2.6 - 3.0 kg b.w.
- Housing: individually in PPO cages (floor area: 2576 cm2) with performed floor.
- Diet: pelleted complete rabbit diet "Altromin 2123" from Altromin, D-32791 Lage, Lippe; ad libitum.
- Water: free access to domestic drinking water acidified with hydrochloric acid to pH 2.5
- Acclimation period: at least 1 week
ENVIRONMENTAL CONDITIONS
- Temperature: 20°C ± 3°C
- Humidity: 55% ± 15%
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): cycle of 12 hours light (06 to 18h) and 12 hours darkness
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted - Duration of treatment / exposure:
- Single exposure without rinsing.
- Observation period (in vivo):
- 1, 24, 48 and 72 hours after treatment
- Number of animals or in vitro replicates:
- 4 females
- Details on study design:
- TESTING PROCEDURE
- The day before testing both eyes of the animals were examined with a hand held inspection lamp fitted with white and UV-light and magnifying glass with 2 x magnification to ensure there were no defects or irritation. The examination was performed before and after instillation of Fluorescein.
- The left eye was treated. The right eye remained untreated and served as control.
- 0.1 ml of the warmed test item was placed in the left eye of the rabbits by gently pulling the lower lid away from the eyeball to form a cup into which the test article was dropped. The lids were then gently held together for 1 second.
- The eyes were examined and the grade of ocular reaction was recorded one hour and 24 hours later. After the first 24 hour reading Fluorescein was instilled. After rinsing with 20 ml 0.9% sodium chloride solution the eyes were examined again using UV-light to detect possible comeal damage.
- The eyes were also examined 48 and 72 hours after the treatment.
EVALUATION OF THE REACTIONS (see tables below)
ASSESSMENT
A substance or a preparation is considered to be irritant if it causes ocular lesions which occur within 72 hours after exposure and which persist for at least 24 hours.
Ocular lesions are significant if the rnean scores of the eye irritation test have any of the following values:
- cornea opacity ≥ 2 but < 3
- iris lesion ≥ 1 but < 1.5
- redness of conjunctivae ≥ 2.5
- oedema of conjunctivae (chemosis) ≥ 2
or, in the case where the test has being completed using three animals if the lesions, on two or more animals, are equivalent to any of the above values except that for iris lesion the value should be equal or greater than 1 but less than 2 and for redness of the conjunctivae the value should be equal or greater than 2.5.
In both cases all scores at each of the reading times (24, 48 and 72 hours) for an effect should be used in calculating the respective mean values.
A substance or a preparation is considered to be able to cause serious damage to eyes if it causes severe ocular lesions which occur within 72 hours after exposure and which persist for at least 24 hours.
Ocular lesions are severe if the means of the scores of the eye Irritation test have any of the values:
- cornea opacity ≥3
- iris lesion ≥ 1.5
The same shall be the case where the test has been completed using three animals if these lesions, on two or more animals, have any of the values:
- cornea opacity ≥ 3
- iris lesion = 2
In both cases all scores at each of the reading times (24, 48 and 72 hours) for an effect should be used in calculating the respective mean values.
Ocular lesions are also severe when they are still present at the end of the observation time.
Ocular lesions are also severe if the substance or preparation causes irreversible colouration of the eyes.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, #2, #3, #4
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: reversibility: not applicable
- Irritation parameter:
- iris score
- Basis:
- animal: #1, #2, #3, #4
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: reversibility: not applicable
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #1, #2, #3, #4
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- chemosis score
- Basis:
- animal: #1, #2, #3, #4
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: reversibility: not applicable
- Irritant / corrosive response data:
- One hour after application of the test article animals No. 1565, No. 1571, No. 1572 and No. 1573 showed some conjunctival vessels definitely injected.
After 24 hours some conjunctival vessels were definitely injected in all animals. After 48 and 72 hours all animals were free of any signs of eye irritation.
Any other information on results incl. tables
Scores for ocular lesions:
*individual mean score: Only the scores from the readings after 24, 48 and 72 hours are included in the calculation of the individual mean scores.
Rabbit No/Weight (kg) | Parameters | Individual mean score* |
1565/2.9 | cornea opacity | 0.00 |
iris | 0.00 | |
conjunctiva redness | 0.33 | |
conjunctiva chemosis | 0.00 | |
1571/2.6 | cornea opacity | 0.00 |
iris | 0.00 | |
conjunctiva redness | 0.33 | |
conjunctiva chemosis | 0.00 | |
1572/2.8 | cornea opacity | 0.00 |
iris | 0.00 | |
conjunctiva redness | 0.33 | |
conjunctiva chemosis | 0.00 | |
1573/3.0 | cornea opacity | 0.00 |
iris | 0.00 | |
conjunctiva redness | 0.33 | |
conjunctiva chemosis | 0.00 |
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No. 1272/2008
- Conclusions:
- The test substance shall not be classified as eye irritating.
- Executive summary:
In the current study the eye irritant effect of the test item was investigated according to the method recommended in the OECD Guideline 405 and EEC Guideline B.5.
Four female albino rabbits were exposed to 0.1 mL of the undiluted test articie in the left eye, while the other eye remained untreated and served as control. The eyes were examined and the changes were graded according to a numerical scale one hour, 24, 48 and 72 hours after dosing.
Slight signs of irritation were observed on the treated eyes, however, these were reversible and according to the criteria set out in the CLP regulation the test item shall not be classified as eye irritant.
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