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EC number: 203-867-5 | CAS number: 111-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 1983
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-(2-aminoethylamino)ethanol
- EC Number:
- 203-867-5
- EC Name:
- 2-(2-aminoethylamino)ethanol
- Cas Number:
- 111-41-1
- Molecular formula:
- C4H12N2O
- IUPAC Name:
- 2-[(2-aminoethyl)amino]ethan-1-ol
- Details on test material:
- - Name of test material: Aminoethylethanolamin (AEEA), test substance 89/233
- Analytical purity: 99 %
- Storage condition of test material: roóm temperature
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- microsomal enzyme systems (S-9 fraction) from Aroclor 1254-induced male Sprague-Dawley rat liver
- Test concentrations with justification for top dose:
- 1st experiment: 0, 20, 100, 500, 2500 or 5000 µg/plate
2nd experiment: 0, 2000, 4000, 6000, 8000 µg/plate - Vehicle / solvent:
- - solvent used: double distilled water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- sterility control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- solvent control
- True negative controls:
- no
- Remarks:
- with and without S-9 mix , in TA 1535, TA 1537, TA 98 and TA 100
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (with S-9 mix)
- Remarks:
- with S-9 mix , in TA 1535, TA 1537, TA 98 and TA 100
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- without S-9 mix , in TA 1535, TA 1537, TA 98 and TA 100
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-phenylendiamine
- Remarks:
- without S-9 mix , in TA 1535, TA 1537, TA 98 and TA 100
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S-9 mix, in TA 1535, TA 1537, TA 98 and TA 100
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
- standard plate and preincubation method
DURATION
- Preincubation period: 20 minutes
- Expression time (cells in growth medium): 48 h
SELECTION AGENT (mutation assays): minimal amino acid solution 0.05 mM histidine + 0.05 mM biotin
NUMBER OF PLATES:
- 3 per dose or control
DETERMINATION OF CYTOTOXICITY
- Method: colonies (his+ revertants) were counted
- Evaluation criteria:
- Characterization of a substance as positive requires:
- doubling of the spontaneous mutation rate
- dose-response relationship
- reproducibility of the results
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 2500 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
AEEA showed no mutagenic response in any strain with or
without S-9 mix.
The substance was completely soluble in water.
Cytotoxicity was only observed in the preincubation test depending on the strain at 2500 and 5000 µg/plate.
Applicant's summary and conclusion
- Executive summary:
In a reverse gene mutation assay the bacteria strains TA 1535, TA 1537, TA 98 and TA 100 of S. typhimurium were exposed to AEEA (99.0 % a.i.) at concentrations of 0, 20, 100, 500, 2000, 2500, 4000, 5000, 6000 or 8000 µg/plate (3 plates/dose) in the presence and absence of mammalian metabolic activation applying both the standard plate and the pre-incubation method (BASF AG, Department of Toxicology, 1991). AEEA was completely soluble in water and tested up to 8000 µg/plate. Cytotoxicity was only observed in the pre-incubation test depending on the strain at 2500 and 5000 µg/plate. The positive controls induced the appropriate responses in the corresponding strains. There was no evidence of induced mutant colonies over background.
This study is classified as acceptable and satisfies the requirement of OECD Test Guideline 471 for in vitro mutagenicity (bacterial reverse gene mutation) data.
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