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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Industrial instead of "predominantly domestic sewage" is used, no GLP certificate

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetoacetanilide
EC Number:
202-996-4
EC Name:
Acetoacetanilide
Cas Number:
102-01-2
Molecular formula:
C10H11NO2
IUPAC Name:
3-oxo-N-phenylbutanamide

Test organisms

Test organisms (species):
activated sludge, industrial

Study design

Test type:
static
Total exposure duration:
3 h

Test conditions

Test temperature:
21°C
pH:
7.0 - 7.3
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
499 mg/L
Nominal / measured:
estimated
Conc. based on:
dissolved
Remarks:
testmaterial
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: calculated
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
898 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
9 375 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 value for bacteria toxicity, determined in the respiration inhibition test with activated sewage sludge indicates a very low toxic potential.