Potassium iodate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of various test chemicals

Justification for type of information:

Experimental data of various test chemicals is from collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is based on three short term toxicity study of test chemical on the fish.

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

2: Sample Storage conditions : Room temperature 30°C

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

1, 2: TEST ORGANISM
- Common name: Zebra fish(Danio rerio)
- Source: Anytime Pet, Jariphataka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.4 cm
- Weight at study initiation (mean and range, SD): 0.045g (average)


ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): Same as test
- Feeding frequency during acclimation: Daily once
- Health during acclimation (any mortality observed): No mortality were observed

Other conditions during acclimation:
- Photoperiod 16 hours light and 8 hours dark
- Temperature range 23.9 °C
- pH range 7.5
- DO range 7.6 mg/l
- Aeration Continous

2: Test species/strain : Zebra Fish (Danio rerio)
Animal breeder/ supplier : Ashish Fish Center
Age of the animals : 3-4 months
Average weight: 0.203 g
Average length: 2.3 cm
Body weight : 0.203 g (approx.)
Diet : Standard brand feed, once daily
Physical conditions: No abnormalities

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72 and 96 hrs

Hardness:

1: 155.5 (mg of CaCO3)

Test temperature:

1: 23 °C
2: 29°C

pH:

1: 7.2
2: Between 7-8

Dissolved oxygen:

1: 7.6 mg/l
2: 7.4

Nominal and measured concentrations:

1: 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L
2: 100 mg/L

Details on test conditions:

1: TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water with 3 liter of headspace.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 2


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light and 8 hours dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations of (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.

2: -Test water: Deionized water was used.
-Conductivity (uS/cm) : 0.5 to 1
-Test vessels: The test vessels used were all glass bowl aquaria of 12” each.
-Photoperiod : 12 hours light period and 12-hour darkness
-Volume of water : 10 liters
-No. of animals per vessel : 8 fishes
-Adaptation : Test conditions corresponded to housing conditions
-Withdrawal of food : 1 day before and during exposure
-Aeration : Aeration was provided 1 day before the starting of experiment

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1st study

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 2nd study

Validity criteria fulfilled:

no

Conclusions:

1: Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra fish (Danio rerio) was determined to be 100 mg/L by observing the mortality.
2: Based on nominal concentrations, the median lethal concentration LC-50 (96 h) for test chemical on the mortality of fishe Danio rerio was observed to be >100 mg/L.
Thus based on the above effects, chemical consider to be nontoxic and not classified.

Executive summary:

Summarized result for the toxicity of test chemical on the mortality of fish were reviewed and mention below:

 

This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Potassium nitrite on Zebra fish (Danio rerio) was determined to be 100 mg/L by observing the mortality. As the effect were observed at borderline 100 mg/l, so we cannot classify the chemical as toxic.

 

Above study was supported by the second study from experimental report. This study was designed to assess the acute toxic effects of the test compound on the Zebra fish (Danio rerio). Bowl aquaria containing 10 liters of deionized water were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1g of the test substance in10 liters deionized water with continuous stirring for achieving the test concentration of 100 mg/L, respectively. The limit test at 100 mg/L was conducted and it was observed that the LC-50 is greater than this concentration. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were found in the control aquaria. The LC0 (96 hours) was observed at 100 mg/L (highest concentration at which no mortality was observed). LC50 (96 hours) was > 100mg/L.

 

Thus based on the above effects, chemical consider to be nontoxic and not classified.

Description of key information

1: Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra fish (Danio rerio) was determined to be 100 mg/L by observing the mortality.

2: Based on nominal concentrations, the median lethal concentration LC-50 (96 h) for test chemical on the mortality of fishe Danio rerio was observed to be >100 mg/L.

Thus based on the above effects, chemical consider to be nontoxic and not classified.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Summarized result for the toxicity of test chemical including structurally and functionally similar read across chemical on the mortality of fish were reviewed and mention below:

 

This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Potassium nitrite on Zebra fish (Danio rerio) was determined to be 100 mg/L by observing the mortality. As the effect were observed at borderline 100 mg/l, so we cannot classify the chemical as toxic.

 

Above study was supported by the second study from experimental report. This study was designed to assess the acute toxic effects of the test compound on the Zebra fish (Danio rerio). Bowl aquaria containing 10 liters of deionized water were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1g of the test substance in10 liters deionized water with continuous stirring for achieving the test concentration of 100 mg/L, respectively. The limit test at 100 mg/L was conducted and it was observed that the LC-50 is greater than this concentration. Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. The test vessels used were all glass bowl aquaria of 12” each. In a 96 h study for test chemical on Danio rerio, at a test concentration of 100 mg/L, the fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l. No mortalities were found in the control aquaria. The LC0 (96 hours) was observed at 100 mg/L (highest concentration at which no mortality was observed). LC50 (96 hours) was > 100mg/L.

 

Thus based on the above effects, chemical consider to be nontoxic and not classified.