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EC number: 413-550-5 | CAS number: 142068-96-0 H112339
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- November 1991 to January 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to EU & OECD test guidance in compliance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- EC Number:
- 413-550-5
- EC Name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- Cas Number:
- 142068-96-0
- Molecular formula:
- Hill formula: C53H28Cl2N14Na8O26S8
- IUPAC Name:
- octasodium 5-[(4-chloro-6-{[4-({[4-chloro-6-({7-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-8-hydroxy-3,6-disulfonatonaphthalen-1-yl}amino)-1,3,5-triazin-2-yl]amino}methyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
- Details on test material:
- H112339 :- The test chemical (reference: NBY 405/74) was obtained from ICI Central Notification Unit, Alderley Park, Macclesfield, Cheshire SK10 4TJ. A Certificate of Analysis (reference 9108127, dated 8 August 1991) stated that the test sample contained 10.5Z w/w water, therefore dose calculations treated the material as 89.5Z w/w technical material. Macroecology ref. 91/78.
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- The solutions were applied to 1.25 kg and 2.5 kg (dry weight equivalents) of artificial soil for the initial and definitive tests, respectively. Soil concentrations for H112339 in the initial test were nominally 0, 1, 10, 100 and 1,000 mg active ingredient (ai) kg-1 artificial soil (dry weight). In the definitive test the soil concentrations were 0, 100 and 1,000 mg ai kg-1 .
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The test soil was a mixture of the following ingredients in oven (approximately 105°C) dry weight proportions:
70% fine silica sand (Grade 110) Supplied by British Industrial Sands, Redhill, Surrey, U.K.
20% kaolinite clay (GTY Powder) Supplied by English China Clay International, London S.W.I.
10% peat (Shamrock Moss) organic matter = 97.7% Supplied by Bord na Mona, Dublin, Eire. Ground then 2mm sieved to remove large plant remains.
Calcium carbonate was incorporated into the soil at 5 g kg-1. Ingredients, in their natural fresh weight form, were mixed in a rotary cement mixer.
The application and incorporation of the H112339 test solution in the artificial soil followed the same procedure for both the tests. The weighed artificial soil was placed into a food mixer bowl and the test solution was added slowly while mixing. The soil was mixed for two minutes by a heavy duty food mixer to evenly incorporate the test substance into the soil. Deionised water was then added to bring the moisture content of the soil up to 50% and mixing continued for a further two minutes. 750 g (fresh weight) aliquots were weighed out into 1 litre glass beakers. For the initial and definitive tests, E. foetida were exposed in two and four aliquots (replicates) of treated soil per treatment, respectively.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- Eisenia foetidaused were clitellate adults that had been cultured in bulb fibre compost (J. Arthur Bower's .Lincoln .England) at Jealott's Hill. The earthworms were supplied by Monkfield, The Aquatic Centre, Cambridge, U.K. The cultures arrived at Jealott's Hill between March and May 1990 and were subsequently held in a controlled environment room.
The mean weight + standard deviation (SD) for E. foetida used in the initial and definitive test was 0.55 + 0.03 g and 0.47 + 0.02 g, respectively.
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Post exposure observation period:
- No post exposure observation period
Test conditions
- Test temperature:
- 20 + 2°C
- pH:
- 6.1 + 0.3
- Moisture:
- 45-52%
- Details on test conditions:
- Preparation of Test Solutions.
H112339 solutions were prepared in deionised water, and made up to 25 ml by the addition of deionised water, for application to the artificial soil. 25 ml of deionised water was used in the preparation of the control soil.
The solutions were applied to 1.25 kg and 2.5 kg (dry weight equivalents) of artificial soil for the initial and definitive tests, respectively. Soil concentrations for H112339 in the initial test were nominally 0, 1, 10, 100 and 1,000 mg active ingredient (ai) kg-1 artificial soil (dry weight). In the definitive test the soil concentrations were 0, 100 and 1,000 mg ai kg-1 .
Chloroacetamide solutions were prepared in deionised water and made up to 50ml for application to artificial soil, to give nominal concentrations of 18, 32 and 56 mg kg-1 (dry weight) as a toxic standard treatment. Chloroacetamide was tested on this batch of E. foetida on 16 August 1991.
Application and Incorporation of Test Solutions in Artificial Soil
The application and incorporation of the H112339 test solution in the artificial soil followed the same procedure for both the tests. The weighed artificial soil was placed into a food mixer bowl and the test solution was added slowly while mixing. The soil was mixed for two minutes by a heavy duty food mixer to evenly incorporate the test substance into the soil. Deionised water was then added to bring the moisture content of the soil up to 50% and mixing continued for a further two minutes. 750 g (fresh weight) aliquots were weighed out into 1 litre glass beakerB. For the initial and definitive tests, E. foetida were exposed in two and four aliquots (replicates) of treated soil per treatment, respectively.
Addition of Test Earthworms
Clitellate E. foetida, which had been preconditioned in untreated artificial soil (50% moisture content) for the previous 24 hours, were added, ten to each beaker. Each batch of ten E. foetida was weighed so that any weight change at the end of the exposure period could be measured. The mean weight and standard deviation of E. foetida used in the test was determined from these weighings. The E. foetida were placed on the surface of the soil between 1 and 2 hours after chemical treatment, and cling film was placed over the top to prevent them from escaping. Six small holes were made in the cling film to allow ventilation.
Test Conditions
The conditions throughout the tests were as follows:
Soil moisture contents were within the range 45-52% throughout the test period (measured at the beginning and end of the tests). Moisture contents were maintained by surface watering with deionised water. The pH of the soils remained within the range 6.1 + 0.3. E. foetida in the test soil were held in a constant environment room at a temperature of 20 + 2°C. A 24 hour light regime was maintained from six fluorescent white tubes. Light intensity was 1,000 + 300 lux.
Assessment of Earthworms
E. foetida were assessed for mortality and any abnormal behavioural effects after 14 days in the initial test and after 7 and 14 days in the definitive test. The soil was tipped onto a clean surface and E. foetida were recorded as dead if there was no response to a mechanical stimulus of their front end. Any E. foetida not found after careful searching of the soil were assumed to be dead. Each batch of ten E. foetida was weighed at the beginning and end of each test to determine any change in bodyweight. - Nominal and measured concentrations:
- Initial test were nominally 0, 1, 10, 100 and 1,000 mg active ingredient (ai) kg-1 artificial soil (dry weight). In the definitive test the soil concentrations were 0, 100 and 1,000 mg ai kg-1.
- Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 other: mg ai kg-1
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 other: mg ai kg-1
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The 14 day LC50 value for H112339 was greater than 1,000 mg ai kg-1 (the highest dose tested). There were no mortalities, behavioural effects nor significant bodyweight changes compared to the control (P=5%) at 1,000 mg ai kg-1 in the definitive test. However, all earthworms in this treatment showed red coloration. This was not considered to be a toxic effect. At 100 mg ai kg-1 there were no deaths, abnormalities in behaviour or external condition, nor were there any significant effects on bodyweight (P=5%). Hence, the No Observed Effect Level was at least 100 mg ai kg-1. There was no control mortality.
- Results with reference substance (positive control):
- The 14 day LC50 value (and 95% confidence limits) for 2-chloroacetamide was 38 (35-43) mg kg-1.
- Reported statistics and error estimates:
- The LC50 value and its 95% confidence limits were estimated for chloroacetamide using the standard technique of iteratively reweighted linear regression of the logit of percent mortality upon log10 (dose).
For H112339, the data on bodyweight were analysed by a one-way analysis of covariance using final (post-treatment) bodyweight as the dependent variable and initial (pre-treatment) bodyweight as a covariate. In this analysis it was assumed that there was a straight line relationship between final and initial bodyweights. The effect of this analysis is to adjust the means of the final bodyweights to a constant value of initial weight so that a meaningful comparison can be made between them.
Using the pooled estimate of error variability from the analysis, t-tests were carried out between the mean final bodyweight (adjusted for the mean initial bodyweight) at each dose with the mean final bodyweight (adjusted for the mean initial bodyweight) in the control.
LC50 estimation was performed using the in-house package LOGITPC Version 1.2. Analysis of covariance was performed using Statistical Analysis System (SAS), Version 6.04.
Any other information on results incl. tables
Initial Test – Mortality and bodyweight ofE. foetidaafter 14 days exposure to H112339 in artificial soil
H112339 Concentration in Soil (mg ai kg-1) |
Replicate |
Numbers dead out of 10 |
Weight of 10E. foetida(g) |
||
14 days |
Start Day 0 |
Finish Day 14 |
% Change |
||
Control |
1 |
0 |
5.8 |
5.6 |
-3.4 |
2 |
0 |
5.8 |
5.4 |
-6.9 |
|
1 mg kg-1 |
1 |
0 |
5.6 |
5.2 |
-7.1 |
2 |
0 |
5.7 |
5.0 |
-12.3 |
|
10 mg kg-1 |
1 |
0 |
5.0 |
4.8 |
-4.0 |
2 |
0 |
5.2 |
4.8 |
-7.7 |
|
100 mg kg-1 |
1 |
0 |
5.4 |
5.1 |
-5.6 |
2 |
0 |
5.9 |
5.3 |
-10.2 |
|
1000 mg kg-1 |
1 |
1 |
5.6 |
5.2a |
-7.1 |
2 |
1 |
5.2 |
5.0a |
.3.8 |
a Where mortality occurred, the end weight and the percentage weight were adjusted to account for the dead earthworm using the following calculation:
End weight for 10 worms (g) =
Weight of remaining worms (g) X No. Exposed (10)/No. Surviving
Definitive Test – Mortality and bodyweight ofE. foetidaafter 7 and 14 days exposure to H112339 in artificial soil
H112339 Concentration in Soil (mg ai kg-1) |
Replicate |
Numbers dead out of 10 |
Weight of 10E. foetida(g) |
|||
7 days |
14 days |
Start Day 0 |
Finish Day 14 |
% Change |
||
Control |
1 |
0 |
0 |
4.4 |
4.3 |
-2.3 |
2 |
0 |
0 |
5.0 |
4.7 |
-6.0 |
|
3 |
0 |
0 |
4.4 |
4.4 |
0 |
|
4 |
0 |
0 |
4.4 |
4.4 |
0 |
|
100 mg kg-1 |
1 |
0 |
0 |
4.7 |
4.5 |
-4.3 |
2 |
0 |
0 |
4.5 |
4.5 |
0 |
|
3 |
0 |
0 |
5.1 |
5.2 |
+2.0 |
|
4 |
0 |
0 |
4.9 |
4.9 |
0 |
|
1000 mg kg-1 |
1 |
0 |
0 |
4.7 |
4.4 |
-6.4 |
2 |
0 |
0 |
4.5 |
4.2 |
-6.7 |
|
3 |
0 |
0 |
4.9 |
4.6 |
-6.1 |
|
4 |
0 |
0 |
4.6 |
4.7 |
+2.2 |
Mortality ofE. foetidaafter 7 and 14 days exposure to 2-chloroacetamide in artificial soil
Chloroacetamide Concentration in Soil (mg kg-1) |
Replicate |
Numbers Dead out of 10 |
|
7 days |
14 days |
||
18 |
1 |
0 |
0 |
2 |
0 |
0 |
|
3 |
0 |
0 |
|
4 |
0 |
0 |
|
32 |
1 |
2 |
2 |
2 |
0 |
0 |
|
3 |
1 |
3 |
|
4 |
1 |
1 |
|
56 |
1 |
10 |
10 |
2 |
10 |
10 |
|
3 |
10 |
10 |
|
4 |
9 |
9 |
Treatment Means ofE. foetidabodyweights after 14 days exposure to H112339
Treatment |
Mean Adjusted Final Bodyweights and (Standard Errors) for Groups of 10 Earthworms |
Control |
4.55 (0.09) |
100 mg ai kg-1 |
4.67 (0.09) |
1000 mg ai kg-1 |
4.48 (0.08) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 14 day LC50 value for H112339 was greater than 1,000 mg ai kg-1 which was the highest dose tested. The No Observed Effect Level was at least 100 mg ai kg-1.
- Executive summary:
Eisenia foetida(clitellate adults) were exposed to concentrations of test substance BT12339 evenly incorporated into an artificial soil for 14 days at 20 + 2°C.
The 14 day LC50 value was greater than 1,000 mg active ingredient (ai) kg-1soil which was the highest dose tested. At 100 mg ai kg-1there were no deaths or significant bodyweight changes (P=5%) nor abnormalities in behaviour or external condition. Thus, the No Observed Effect Level was at least 100 mg ai kg-1. The only effect at 1,000 mg ai kg-1in the definitive test was the red coloration of the earthworms which was not considered to be a toxic effect.
The test substance is not considered to pose a hazard to soil macro-organisms.
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