Registration Dossier
Registration Dossier
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EC number: 251-410-3 | CAS number: 33229-34-4
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
The potential of HC Blue no 2 to induce contact hypersensitivity was examined using the local lymph node assay (OECD guideline 429). Groups of 4 female mice in each were treated with 0.5%, 1%, 2.5%, 5% and 10% of the test substance in vehicle (dimethylformamide) for 3 daily treatments. After 2 days of resting, the proliferation of lymphocytes in the lymph node draining the application site was measured by incorporation of tritiated methyl thymidine (day 6). While the data is equivocal, the test substance HC Blue No 2 is considered to have a skin sensitising potential.
Migrated from Short description of key information:
The potential of HC Blue no 2 to induce contact hypersensitivity was examined using the local lymph node assay (OECD guideline 429). Groups of 4 female mice in each were treated with 0.5%, 1%, 2.5%, 5% and 10% of the test substance in vehicle (dimethylformamide) for 3 daily treatments. After 2 days of resting, the proliferation of lymphocytes in the lymph node draining the application site was measured by incorporation of tritiated methyl thymidine (day 6). While the data is equivocal, the test substance HC Blue No 2 is considered to have a skin sensitising potential.
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
