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EC number: 429-790-9 | CAS number: 137281-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 to 24 December 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP, guideline study was followed
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-[2-(2-amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoic acid
- EC Number:
- 429-790-9
- EC Name:
- 4-[2-(2-amino-4,7-dihydro-4-oxo-3H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoic acid
- Cas Number:
- 137281-39-1
- Molecular formula:
- Hill formula: C15 H14 N4 O3 CAS formula: C15 H14 N4 O3
- IUPAC Name:
- 4-(2-{2-amino-4-oxo-3H,4H,7H-pyrrolo[2,3-d]pyrimidin-5-yl}ethyl)benzoic acid
- Test material form:
- solid: particulate/powder
- Details on test material:
- Name: Compound 202723
Storage condition of test material: Room temperature in dark
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of newly prepared test solutions were taken on Days 0, 2, 6, 8, 13, 15, and 20. Samples of aged solutions were taken on Days 1, 3, 7, 9, 14, 16, and 21. Three QC samples were prepared at each interval and remained with samples.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material weighed (weight corrected for purity) and dissolved in dilution water to prepare a 10 mg/L solution (prepared daily)
- Controls: Dilution water only
- No evidence of undissolved material
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: On site cultures
- Feeding during test: 200 µL algal suspension (Ankistrodesmus falcatus, 4 x 10+7 cells/mL) and 50 µL of a yeast, cereal leaves and digested flaked fish food suspension
- Feeding frequency: Daily
SOURCE AND AGE OF TEST ORGANISMS:
Test organisms were not from the first brood and were derived from healthy culture stock. Test organisms were < 24 hours old at test initiation
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- Total hardness range as calcium carbonate (CaCO3) of 170 to 180 mg/L, a total alkalinity range (as CaCO3) of 100 to 110 mg/L
- Test temperature:
- 18 to 21 ºC (continuous monitoring)
- pH:
- pH range of 7.8 to 8.6
- Dissolved oxygen:
7.3 to 10.0 mg/L- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal 10 mg compound 202723/L
Mean Measured: 10 mg compound 202723/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL open glass beakers (volume 80 mL)
- Aeration: None
- Type of exposure: Static-renewal
- Renewal rate of test solution: 100% solution change daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well-water, autoclaved, fortified with salts and nutrients to prepare hard water, and mixed overnight while aerating
- Total organic carbon: 0.69 mg/L
- Metals: None detected at toxic concentrations
- Pesticides: None detected at toxic concentrations
- Alkalinity: 100 to 110 mg/L as CaCO3
- Conductivity: 500 to 650 µmhos/cm
- Culture medium same as test medium:
- Intervals of water quality measurement: Dissolved oxygen, temperature and pH measured at each renewal period in new and aged solutions. Alkalinity and conductivity measured weekly.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h darkness
- Light intensity: 8.6 to 11 µE per m2 per s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Number of immobilized daphnids measured daily
Numbers of offspring determined upon first brood release in any vessel and daily throughout remainder of test
Number of immobilized offspring recorded
VEHICLE CONTROL PERFORMED: no
TEST CONCENTRATIONS
-Single concentration of 10 mg compound 202723/L (nominal)
-Justification for using less concentrations than requested by guideline: limit test
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- There was acceptable survival (>80%) and reproduction (>60 offspring per female) in the controls in the study. No effects due to exposure to compound
202723 were observed (see Table 1). - Reported statistics and error estimates:
- Statistical analysis (t-Test) indicated no significant difference between the control and treatment group for reproduction and length.
Any other information on results incl. tables
Table 1: Mean survival, reproduction and total body lengths of parental daphnids at termination o fthe 21 day static renewal toxicity test exposing daphnids (Daphnia magna) to compound 202723
Mean Measured Conc (mg compound 202723/L) | Mean percent survival | Mean Reproduction (SD) | Total Body Length in mm (SD) |
Control | 100 | 128 (18) | 4.51 (0.11) |
10mg | 100 | 122 (17) | 4.61 (0.1) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Study is considered valid as the performance criteria defined in OECD211 were met. There was acceptable survival (>80%) and reproduction (>60 offspring per female) in the controls in the study.
Results from an acute aquatic study on invertebrates is not available for the test material. The compound is highly likely to be of low acute toxicity to invertebrates based on a lack of toxicity demonstrated in this chronic invertebrate study. The chronic invertebrate test was conducted as a limit test using a test concentration of 10 mg/L and a control group according to OECD 211 guideline. - Executive summary:
Test Substance Compound 202723
Test organism Daphnia magna Test Conditions 21 day duration, daily test solution renewals, temp range of 18 to 21oC, photoperiod of 16 hours light at an illumination range of 8.6 to 11uE.m-2.s-1 and 8 hours dark. Dilution water Fortified well water pH 8.1 to 8.5, Dissolved oxygen: 7.4 to 9.4mg/l, Specific conductivity 600umhos/cm, total hardness as Ca CO3: 170 to 180 mg/L, total alkalinity as CaCO3: 100 to 110mg/L Results No effects were found on survival, growth or reproduction among daphnids exposed to compound 202723 at 10mg/L. The NOEC for compound 202723 is 10 mg/l
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