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Diss Factsheets
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EC number: 203-604-4 | CAS number: 108-67-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, near guideline, experimental study, published in peer reviewed literature; minor restrictions in design and/or reporting but otherwise adequate for assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412, Part 9 (draft standard) 1988
- Deviations:
- yes
- Remarks:
- Modified procedure for volatile substances. 250 mL bottles used with ground-glass stoppers and with an overall test time of 48 hours.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- no
- Details on sampling:
- No data reported
- Vehicle:
- not specified
- Details on test solutions:
- In order to produce the test solutions, water-soluble substances were quantitatively dissolved to produce an optically clear stock solution in 800 mL double-distilled water using magnetic stirrers (max 24 hours). Before beginning the test, the stock solutions of the substances to be tested were adjusted to pH 8.0±0.3 whereby the concentration of the acid or base was selected so as to ensure that the volume added remained small. When testing volatile substances (e.g. 1,3,5-trimethylbenzene) the procedure was modified and 250 mL wide-neck bottles with ground-glass stoppers were used. The parallel dilution series (f=2) was set up in the following way: the volume of dilution water (e.g. 27.5 mL) corresponding to the dilution level (e.g. 1:4 of the test medium) was pre-filled into each of the six bottles for the conentration level. Then, 5 mL of the 10-fold concentration of the nutrient solution and the corresponding volume of the test preparation (stock solution) (e.g. 12.5 mL) for the desired concentration was added to each test vessel. The bottles were closed immediately. Finally, the stopper was lifted briefly to add 5 mL of the algae suspension of the preliminary culture.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Strain number 8681 SAG. A strain that has been maintained over a number of decades in submerged culture. For this purpose, the required number of 100 mL Erlenmeyer flasks with metal caps containing 20 mL nutrient solution were sterilised in a steam steriliser for 30 minutes on 2 consecutive days. After cooling, the contents of each flask were inoculated with 2 mL cell suspension taken from a 10 day old stock culture. The inoculated flasks were placed on a white surface, protected from daylight and exposed to constant light from two parallel fluorescent Osram 40W/30 tubes, at 24 ± 1°C and relative humidity of 50%. To maintain the test strain, fresh stock cultures were prepared at 10 day intervals.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No data reported
- Hardness:
- No data reported
- Test temperature:
- 24 ± 1°C
- pH:
- 8.0± 0.3
- Dissolved oxygen:
- No data reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal tested concentration range 0.40 - 50 mg/L
- Details on test conditions:
- Constant lighting provided by OSRAM l 25/40 fluorescent tubes; each of the positions for the test preparation had an irradiance E = 17.0 W/m2. Temperature and arradiance are given as manipulated variables.
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 8.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Assumed to be extrapolated
- Details on results:
- No data reported
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The area under the growth curve (integral of biomass with time) was calculated according to the given equation and from this was calculated the percentage inhibition of cell multiplication during the test for each concentration level on the basis of a comparison of the biomass formed under the influence of the substance with the biomass in the control population.
The average growth rate for cultures showing exponential growth was calculated and on this basis, the growth related inhibition was calcualted from the difference between the growth rates under the influence of the substance and the growth rate in the control. The growth rate, as stipulated by DIN 38 312 Part 9 in the test procedure, was expressed by means of the logarithm of the change in the cell count.
The tested concentration was assigned to the respective inhibition values in the probability paper. The regression line was determined and from this could be read the desired EC10 and EC50 values. - Validity criteria fulfilled:
- yes
- Remarks:
- 16 fold increase in cell growth confirmed for control in 48 hours, meets DIN 38 412 part 9 criteria.
- Conclusions:
- The reported experimental 48 hour EC50 for Scenedesmus subspicatus was determined to be 25 mg/L based on biomass and 53 mg/L based on growth rate. The 48 hour EC10 was determined to be 8.1 mg/L based on biomass and 16 mg/L based on growth rate.
- Executive summary:
This robust summary has a reliability rating of 2 because it is a non-GLP, near guideline, experimental study, published in peer reviewed literature; minor restrictions in design and/or reporting but otherwise adequate for assessment. Since there is no information in the article to suggest that the data are invalid it can be considered suitable as the key study for this endpoint. The reported experimental 48 hour EC50 for Scenedesmus subspicatus was determined to be 25 mg/L based on biomass ad 53 mg/L based on growth rate. The 48 hour EC10 was determined to be 81 mg/L based on biomass and 16 mg/L based on growth rate. The result does not indicate that 1,3,5-trimethylbenzene (mesitylene) would meet the criteria for toxic in the PBT assessment. This endpoint will not affect the classification of the substance since a lower LC50 is found for Daphnia magna which will determine the classification.
Reference
Description of key information
The reported experimental 48 hour EC50 for Scenedesmus subspicatus was determined to be 25 mg/L based on biomass and 53 mg/L based on growth rate. The 48 hour EC10 was determined to be 8.1 mg/L based on biomass and 16 mg/L based on growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 53 mg/L
Additional information
The Kuhn and Pattard (1990) study is a non-GLP, near guideline, experimental study, published in peer reviewed literature with minor restrictions in design and/or reporting but otherwise adequate for assessment. Since there is no information in the article to suggest that the data are invalid, it can be considered suitable as the key study for this endpoint. The 48 hour EC50 value for green algae (Scendesmus subspicatus) was 25 mg/L based on biomass and 53 mg/L based on growth rate. The 48 hour EC10 value was 8.1 mg/L based on biomass and 16 mg/L based on growth rate.
A QSAR calculation using the ECOSAR model gives an estimated 96 hour EC50 for green algae of 3.19 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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