Bis(1-oxotetradecanyloxyphenyl)phenylmethane

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fraunhofer IME

Test material

Test material information

Constituent 1

Reference substance name:

2-{phenyl[2-(tetradecanoyloxy)phenyl]methyl}phenyl tetradecanoate

EC Number:

941-526-9

Molecular formula:

C47H68O4

IUPAC Name:

2-{phenyl[2-(tetradecanoyloxy)phenyl]methyl}phenyl tetradecanoate

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 61.81 SAG
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Göttingen, Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22 +- 2 °C

pH:

Control: 7.48 at test start, 7.81 at test end
Test cultures: initial pH 7.58 - 7.66, at test end 8.15 – 8.34

Nominal and measured concentrations:

0, 6.25, 12.5, 25.2, 50 and 100 mg/L (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL conical glass flasks
- Type:covered with air-permeable siliconesponge caps
- Initial cells density: 10 000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 8

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: pH measured in one test vessel per test concentration including the control. At the end of the test, the solution pH values were measured directly in test vessels. The test culture temperature was measured in one test vessel only at test start

OTHER TEST CONDITIONS
- Photoperiod: continuous illimination
- Light intensity and quality: 8127 - 8221 lux

EFFECT PARAMETERS MEASURED:
Cell concentrations were determined with an electronic particle counter at test start and after 24, 48 and 72 h

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Remarks on result:

other: No growth inhibition observed

Details on results:

No chemical analysis was performed and thus the assessment was based on the nominal concentrations. --> Water accomodated fraction (WAF) used

Results with reference substance (positive control):

EC50 > 100 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No growth inhibition observed