Benzene, C15-16-alkyl derivs.

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented, peer-reviewed publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzene, C10-13-alkyl derivs.

EC Number:

267-051-0

EC Name:

Benzene, C10-13-alkyl derivs.

Cas Number:

67774-74-7

IUPAC Name:

undecylbenzene

Constituent 2

Reference substance name:

Undecylbenzene

EC Number:

229-806-2

EC Name:

Undecylbenzene

Cas Number:

6742-54-7

IUPAC Name:

undecylbenzene

Details on test material:

- Molecular weight (if other than submission substance): 239.6
- Composition of test material, percentage of components: 0.9% C9 or less, 9.4% C10, 40.2% C11, 32.7% C12, 15.9% C13, 0.9% C14, 17% 2 Ph alkanes, <0.5% tetralines

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

no information

Test solutions

Vehicle:

no

Details on test solutions:

A test solutions was prepared, without use of solvent, to reach the solubility concentration (0.041 mg/1).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: laboratory bred
- Age at study initiation (mean and range, SD): less than 24 hrs

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20 degree C

Salinity:

0.2 g/L NaHCO3
0.224 g/l CaCl2
0.026 g/L K2SO4

Nominal and measured concentrations:

nominal: 0, 12.5%, 25%, 50%, 75%, saturated (0.041 mg/L)

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilization

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.041 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: > water solubility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.041 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: > water solubility

Details on results:

No dose-response increase in the number of immobilized daphnids was seen. Also no significant increase in immobilization over controls was seen.

Any other information on results incl. tables

Immobilized daphnids at 48 hrs ¿ LAB

Concentration	1	2	3	4
Control	0	1	0	0
Saturated	0	1	0	0
75%	1	0	0	1
50%	1	0	0	0
25%	0	1	0	0
12.5%	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hr EC50 for daphnids was > 0.041 mg/L.

Executive summary:

In cases where no data were available on the target substance, Benzene, C15 -16 -alkyl derivs., data were read across from a structurally related material (the test substance).

This study determined the short-term toxicity of linear alkyl benzenes to Daphnia magna. Daphnids were exposed to concentrations of 0, 12.5%, 25%, 50%, 75%, and 100% saturated test solution for 48 hrs. At the end of this period, the daphnids were observed for mobility. Though immobilized daphnids were seen at 4 test concentrations, there was no dose-related increase in immobilization, nor was the number of immobilized daphnids significantly increased over negative controls. The 48 -hr EC50 is >0.041 mg/l, this is greater than the water solubility for the test substance.