Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 604-603-5 | CAS number: 147769-93-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17.03.1999 - 16.09.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in GLP compliance and in accordance with an OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- AGEE 623 Amine
- IUPAC Name:
- AGEE 623 Amine
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): AGEE 623 Amine
- Physical state: liquid
- Analytical purity: 80.6%
- Composition of test material, percentage of components: batch contains about 20% solvent
- Purity test date: CoA date: 20.05.1998
- Lot/batch No.: T29844
- Expiration date of the lot/batch: 31.12.1999
- Stability under test conditions: for at least 48 hours
- Storage condition of test material: in original container, at approximate1y + 4 °C, in the dark
- Other:
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Sex: female
- Strain: clone 5
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age at study initiation (mean and range, SD): 7 - 22 hours old
ACCLIMATION
- Acclimation conditions (same as test or not): for 7 hours under test conditions
Study design
- Test type:
- not specified
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- water Hardness: 2.5 mmol/L (= 250.0 mg/L) as CaC03
- Test temperature:
- 21- 22 °C
- pH:
- pH 7.6 to 7.9
- Dissolved oxygen:
- at least 7.4 mg/L or higher
- Salinity:
- CaCI2 x 2H2O: 2.0 mmol/L
MgSO4 X 7H20: 0.5 mmol/L
NaHC03: 0.75 mmol/L
KCI: 0.075 mmol/L - Nominal and measured concentrations:
- measured: 1.0, 2.0,4.0, 8.0 and 16.5 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers of 100 mL volume with 50 mL testmedium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (tested twice per year)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: lower 95% confidence value: 2.5 mg/L / higher 95% confidence value: 4.4 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Control: The experiment is valid because the mortality of Daphnia magna in the control (reconstituted water) did not exceed 10 % and no Daphnia were trapped at the water surface.
- Reported statistics and error estimates:
- The 24-hour and 48-hour EC 50 and the 95 % confidence
Iimits were calculated by the Moving average interpolation.
The NOEC, EC 0 and EC 100 were determined directly from
the raw data.
Any other information on results incl. tables
Signs of lntoxication after 24 hours:
After 24 hours in the control and in the test concentrations
up to 4.0 mg test substance/L no mortality or immobilization
of the test animals was observed (see Table 1). At the
test concentration of 8.0 mg test substance/L immobilization
rate of 30 % was determined after 24 hours. At the
highest test concentration of 16.5 mg test substance/L 75%
of the Daphnia were immobile after 24 hours test duration.
Results after 24 hours:
The 24-hour EC 50 of the test substance was calculated to be 12.4 mg test substance/L with 95 % confidence Limits from 7.6 to 20.3 mg/L. The 24-hour EC 0 was 4.0 mg test substance/L, the 24-hour EC 100 was higher than 16.5 mg test substance/L.
Signs of Intoxication after 48 hours:
After 48 hours of exposure the toxicity of the test substance to Daphnia magna had increased. In the control and up to and including the test concentration of nominal 1.0 mg test substance/L no significant immobility or mortality of the test animals or other signs of intoxication were determined
during the test period of 48 hours. At the test concentration
of nominal 2.0 mg test substance/L 5 test animals were
immobile at the observation after 48 hours (immobilization
rate of 25 % ). At the next high er test concentration of
4.0 mg test substaueeiL 55 % immobilization was determined.
At the two highest test concentrations of 8.0 and
16.5 mg test substance/L all Daphnia were dead after
48 hours test duration.
Results after 48 hours:
The 48-hour EC 50 was calculated to be 3.3 mg test substaueeiL with 95 % confidence Iimits from 2.5 to 4.4 mg/L. The 48-hour EC 0 and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of AGEE 623 Amine was 1.0 mg test substance/L, since no significant immobilization rate and no other signs of intoxication were observed at the test animals up to and including this test concentration. The 48-hour EC 100 amounted to 8.0 mg test substance/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC 50 was calculated to be 3.3 mg test substance/L
with 95 % confidence Iimits from 2.5 to 4.4 mg/L.
The 48-hour EC 0 and the 48-hour NOEC (highest concentration
tested without toxic effects after 48 hours) of
AGEE 623 Amine was 1.0 mg test substance/L, since no
significant immobilization rate and no other signs of intoxication
were observed at the test animals up to and including
this test concentration. The 48-hour EC 100
amounted to 8.0 mg test substance/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.