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EC number: 250-748-9 | CAS number: 31643-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental started 18th June 2007 and finished 17th July 2007. Study Report date 26th July 2007.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study was conducted in a facility operating to GLP within the UK national GLP monitoring programme, but the study report was not audited by the QA unit at the facility and therefore no formal claim of GLP compliance can be made for this study. The analytical purity of the test sample was unknown although expected to be typical of manufacture. The study was reported as a summary report, but is considered an accurate record of the study and its outcome.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- not specified
- Principles of method if other than guideline:
- The test material was assessed for its skin sensitising potential using the Local Lymph Node Assay in the mouse. The test is designed to assess the skin sensitising potential (delayed type hypersensitivity) of the test material following topical application to the dorsal surface of the ear. Primary lymphocyte proliferation is assessed during the sensitising (induction) phase of the response. The assay determines the level of lymphocyte proliferation in lymph nodes draining the application site of the test material. Determination of lymphocyte proliferation is quantified by measuring the incorporation of radiolabelled thymidine in the dividing lymph node cells. The proliferation response of lymph node cells was expressed as the number of radioactive disintegrations per minute per lymph node (dpm/node) and as the ratio of HTdR incorporation into lymph node cells of test nodes relative to that recorded for the control nodes (Stimulation index).
- GLP compliance:
- no
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 5-nitrobenzene-1,2-dicarbonitrile
- EC Number:
- 250-748-9
- EC Name:
- 5-nitrobenzene-1,2-dicarbonitrile
- Cas Number:
- 31643-49-9
- Molecular formula:
- C8H3N3O2
- IUPAC Name:
- 5-nitrobenzene-1,2-dicarbonitrile
- Details on test material:
- The following is taken from the test submission form and is not reported in the summary study report:
- Physical state: solid, crystalline
- Analytical purity: unknown
- Lot/batch No.: Grindley's NBZ 6012/22
- Colour: pale yellow
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 19-20 g
Study design: in vivo (LLNA)
- Vehicle:
- dimethylformamide
- Concentration:
- 10%, 25% and 50% w/w of test material
- No. of animals per dose:
- 4 animals per test concentration and 4 control animals
- Details on study design:
- RANGE FINDING TESTS:
No signs of systemic toxicity at a test conc. of 50% w/w in the preliminary sighting study.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Control (dimethyl formamide): n/a 5% w/w : 2.52 10% w/w: 2.50 25% w/w: 1.75
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- Control (dimethyl formamide): 5424.56 (678.07 per node) 5% w/w : 13645.52 (1705.69 per node) 10% w/w: 13542.99 (1692.87 per node) 25% w/w: 7101.85(1183.64* per node) * Dpm/node obtained by dividing the Dpm vlaue by 8 ( total number of lymph nodes due to one mortality)
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test material was considered to be a non-sensitiser under the conditions of the test.
- Executive summary:
Introduction
The study was performed to assess the potential of the test material to produce delayed type hypersensitivy in the mouse using Method B42 Skin Sensitisation (Local Lymph Node Assay) of Commission Directive 2004/73/EC.
Method
The study is an indication for use as the first stage in the assessment of skin sensitisation potential. Three groups, each of four animals were treated with 50 ul of the test material ( 25ul per ear) as a solution in dimethyl formamide at concentrations of 5%, 10%,25%.
Results
Simulation indices:
5% w/w : 2.52
10% w/w: 2.50
25% w/w: 1.75
Conclusion
The test material was considered to be a non-sensitiser under the conditions of the test.
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