2,2-difluoro-1,3-benzodioxole

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2-difluoro-1,3-benzodioxole

EC Number:

216-431-4

EC Name:

2,2-difluoro-1,3-benzodioxole

Cas Number:

1583-59-1

Molecular formula:

C7H4F2O2

IUPAC Name:

2,2-difluoro-1,3-benzodioxole

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Doses:

2000 mg/kg b.w.

No. of animals per sex per dose:

3

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

One animal died immediately after administration due to a technical failure. All other animals survived until the end of the study period.

Clinical signs:

Slightly ruffled fur was noted in all animals at the 1- hr observation and persisted up to test day 3 in all animals of the second treated group and up to test day 4 in the two remaining animals of the first treated group. Hunched posture was additionally noted from the 2-hr reading to test day 3 in the first treated group and from the 1-hr reading to test day 2 in the second treated group.

Body weight:

within the range.

Gross pathology:

The appendix of the caecum of one animal was blue-red to violed discolored, with hyperemic blood vessels. The appendix was empty while the main part of the caecum was full. Injury of the trachea was noted at the unscheduled necropsy from the animal which died immediately after treatment and permitted to conclude about a technical failure. No macroscopic findings were recorded in the other animals.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The median lethal dose of the test item after single oral administration to female rats, observed over a period of 14 days is greater than 2000 mg/kg b.w..