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EC number: 212-572-0 | CAS number: 827-52-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented GLP study according to international guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
- Version / remarks:
- adopted 21 September 1998
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- up-and-down procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Cyclohexylbenzene
- EC Number:
- 212-572-0
- EC Name:
- Cyclohexylbenzene
- Cas Number:
- 827-52-1
- Molecular formula:
- C12H16
- IUPAC Name:
- cyclohexylbenzene
- Details on test material:
- - Name of test material (as cited in study report): phenylcyclohexane
- Description: clear colourless liquid
- Date received: 2001-08-06
- Storage conditions: room temperature, in the dark
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Sprague-Dawley CD (Crl: CD® (SD) IGS BR)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River (UK) Ltd, Margate, Kent, UK
- Age at study initiation: approximately 8 weeks
- Weight at study initiation: ≥ 200g
- Fasting period before study: overnight immediately before dosing
- Housing: females individually and males in group of 3 in solid-floor polypropylene cages furnished with woodflakes
- Diet: ad libitum (Rat and Mouse Expanded Diet No. 1, Special Diets Services Limited, Witham, Essex, UK) with exception of an overnight fast immediately before dosing and approximately 3 to 4 h after dosing
- Water: ad libitum
- Acclimation period: ≥ 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25
- Humidity (%): 30-70
- Air changes (per hr): ≥ 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Observations for deaths or overt signs of toxicity 1/2, 1, 2 and 4 h after dosing and subsequently once daily for 14 days. Individual body weights were recorded prior to dosing and 7 and 14 days after treatment.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No deaths
- Clinical signs:
- other: Hunched posture was noted in 2 females. Signs of systemic toxicity also noted in one of these females were decreased respiratory rate, laboured respiration, ataxia and emaciation. These 2 females recovered 2 or 6 days after dosing. There were no signs of
- Gross pathology:
- No abnormalities
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute oral median lethal dose (LD50) in the Sprague-Dawley CD (Crl CD® (SD) IGS BR) strain rat was found to be greater than 2000 mg/kg bw.
- Executive summary:
The study was performed to assess the acute oral toxicity of the test material following a single oral administration in the Sprague-Dawley CD (Crl CD® (SD) IGS BR) strain rat. The study was carried out according to OECD guideline 425.
One fasted female animal was treated with the test material at dose level of 2000 mg/kg. A second and a third fasted female rat were also treated at a dose level of 2000 mg/kg. This was then followed by a group of 3 fasted male rats at the same dose level.
The test material was administered orally undiluted. Clinical signs and body weight development were monitored during the study. All animals were subjected to gross necropsy.
There were no deaths. Hunched posture was noted in 2 females. Signs of systemic toxicity also noted in one female were decreased respiratory rate, laboured respiration, ataxia and emaciation. There were no signs of systemic toxicity noted in one female and all males.
All animals showed expected gains in body weight over the study period. No abnormalities were noted at necropsy.
The acute oral median lethal dose (LD50) was found to be greater than 2000 mg/kg bw.
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