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EC number: 236-690-7 | CAS number: 13464-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed to guidelines and to GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hydrazine nitrate
- IUPAC Name:
- Hydrazine nitrate
- Reference substance name:
- Hydrazinium nitrate
- EC Number:
- 236-690-7
- EC Name:
- Hydrazinium nitrate
- Cas Number:
- 13464-97-6
- Molecular formula:
- H4N2.HNO3
- IUPAC Name:
- hydrazinium nitrate
- Details on test material:
- - Name of test material (as cited in study report): Hydrazine nitrate
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Arochlor induced rat liver S9 homogenate
- Test concentrations with justification for top dose:
- 0.03-3.0 mg/plate (0.32-30 mM).
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-anthramine dissolved in DMSO, diluted with DDH2O
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS:3
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index - Evaluation criteria:
- Appearance of background lawn of bacterial growth.
- Statistics:
- Mean and standard deviation
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No precipitation was observed in any of the chemical dilutions.
RANGE-FINDING/SCREENING STUDIES: 5 log concentrations (0.0005-5.0 mg/plate) were tested in TA 98, TA 100 and TA 102 with modified standard plate incorporation, the pre-incubation method. No precipitation was observed in any of the chemical dilutions.
ADDITIONAL INFORMATION ON CYTOTOXICITY: At 3 mg/plate, toxicity was observed in TA98, TA100, and TA 1537 with and without S9 and for TA 102 and TA 1535 without S9. 1 mg/plate was toxic to all strains without S9 activation. - Remarks on result:
- other: strain/cell type: TA 1535 and TA 102
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
Hydrazine nitrate was positive for mutagenicity when tested with metabolic activation. - Executive summary:
In a reverse gene mutation assay in bacteria, strains TA98, TA100, TA102, TA1535 and TA 1537 of S. typhimurium were exposed to hydrazine nitrate, at concentrations of 0.03 -3 mg/plate in the presence and absence of mammalian metabolic activation.
The positive controls induced the appropriate responses in the corresponding strains. There was evidence of induced mutant colonies over background for the test substance.
This study is classified as acceptable. This study satisfies the requirement for Test Guideline EPA OTS 798.5265 for in vitro mutagenicity (bacterial reverse gene mutation) data.
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