2-thiazolidinylidenecyanamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

427-720-1

EC Name:

-

Cas Number:

26364-65-8

Molecular formula:

C4H5N3S

IUPAC Name:

[(1,3-thiazolidin-2-ylidene)amino]formonitrile

Details on test material:

name: Cyanamide, (4,5-dihydroxy-2-thiazolyl)-
molecular formula: C4 H5 N3 S
molecular weight: 127.2
physical state: solid
analytical purity: 98 %

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout (Oncorhynchus mykiss)
- Source: G. Muller, D-37186, FRG
- Length at study initiation (length definition, mean, range and SD): 5.7 + 0.4 cm
- Weight at study initiation (mean and range, SD): 2.1+ 0.5 g
- Food type: commercial trout diet (Brutfutter FB50, Kronen-Fischkraftfutter, D-46483 Wesel, FRG

ACCLIMATION
- Acclimation period: 48 hours
- Acclimation conditions: same as test
- Type and amount of food: commercial trout diet, see above

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

40 - 60 mg CaCO3/l

Test temperature:

12.3°C - 12.9°C

pH:

6.9-7.1

Dissolved oxygen:

88 % - 102 %

Nominal and measured concentrations:

nominal : 102 mg/l
measured : 101 - 103 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0,5 g fish/l

TEST MEDIUM / WATER PARAMETERS
Reconstituted water prepared by adding salt stock solutions to demineralized water
(conductivity < 0.2 uS/cm) to yield the following ionic concentrations (according to ISO) was used:
Ca++ 0.384 mmole/l
Mg++ 0.096 mmole/l
Na+ 0.148 mmole/l
K+ 0.015 mmole/l
Cl- 0.783 mmole/l
HC03- 0.148 mmole/l
S04-- 0.096 mmole/l

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal concentration in mg test substance (pure intermediate)/l	3h		24 h		48 h		72 h		96 h
	Dead	Obs.	Dead	Obs.	Dead	Obs.	Dead	Obs.	Dead	Obs.
control	0	0	0	0	0	0	0	0	0	0
102(100)	0	0	0	0	0	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance is not subject to classification.

Executive summary:

The effect threshold and the lethal treshold (96h) of the test substance 2-CYANIMINO-l,3 -THIAZOLIDINE (CIT), an intermediate for the production of the insecticide YRC 2894 with a purity of 98.0 % to Rainbow Trout (Oncorhynchus mykiss) in a static 96-hour-limit-test was determined to be >102 (>100) mg test substance (a.i.)/l.

In this test one group often fish was exposed to nominal concentration of 102(100) mg test substance

(pure intermediate)/!. One additional group often fish was exposed to untreated test water. There were

neither mortalities nor symptoms of intoxication in the control group.

In the tested concentration at 102(100) mg test substance (pure intermediate)/l no fish showed any

symptoms.

The test substance was homogeneously mixed into the aquarium. Under test conditions the test

substance was stable. Analysis resulted in values between 101 % at day 0 and 103 % at day 4 (based on

analytical determination of 2-CYANIMINO-1,3-THIAZOLIDINE (CIT) in water by HPLC of nominal.

All reported results are related to nominal concentration of the test substance.