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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from a secondary source.
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Toxicity to microorganism study was carried out for time period of 5 hrs.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: (0, 500, 1000, 1500 and 2000 ul/l),
- Sampling method: Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content. At the end of testing HCl was added to stop biochemical processes.
- Vehicle:
- no
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5 h
- Test temperature:
- 25 °C
- Nominal and measured concentrations:
- (0, 500, 1000, 1500 and 2000 ul/l),
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Four 100-ml von Loh bottles
- Type (delete if not applicable): closed, glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations were sealed without air.
- Aeration: No
- Control vessel details : Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content. - Reference substance (positive control):
- not specified
- Duration:
- 5 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 520 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Duration:
- 5 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 520 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on effect on growth inhibition of test bacteria, the 5 hrs EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l.
- Executive summary:
Toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from a secondary source.
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Toxicity to microorganism study was carried out for time period of 16 hrs.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Bacteria
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
- Reference substance (positive control):
- not specified
- Duration:
- 16 h
- Dose descriptor:
- IC50
- Effect conc.:
- 435 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on growth inhibition of bacteria, the 16 hrs IC50 value was determined to be 435 mg/l.
- Executive summary:
Toxicity to microorganism study was carried ot for time period of 16 hrs. Bacteria was used as test innoculum. Based on growth inhibition of bacteria, IC50 value was determined to be 435 mg/l. Based on IC50 value test chemical was considered as non-toxic to microorganism.
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Toxicity to microorganism study was carried out for time period of 16 hrs.
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- WoE-3 : - Concentrations: (0, 500, 1000, 1500 and 2000 ul/l),
- Sampling method: Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content. At the end of testing HCl was added to stop biochemical processes.
- Vehicle:
- no
- Test organisms (species):
- other: WoE-2 : Other bacteria WoE-3 : Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 25 °C
- Nominal and measured concentrations:
- WoE-3 : (0, 500, 1000, 1500 and 2000 ul/l),
- Details on test conditions:
- WoE-3 :
TEST SYSTEM
- Test vessel: Four 100-ml von Loh bottles
- Type (delete if not applicable): closed
- Aeration: No - Reference substance (positive control):
- yes
- Remarks:
- WoE-3 :Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content
- Duration:
- 16 h
- Dose descriptor:
- IC50
- Effect conc.:
- 435 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Duration:
- 5 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 520 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.
- Executive summary:
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganism. The studies are as mentioned below:
First study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.
Second study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.
Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.
Based on above information test chemical was considered as non-toxic to microorganism.
Referenceopen allclose all
Description of key information
Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 520 mg/L
Additional information
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganism. The studies are as mentioned below:
First study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.
Second study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.
Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.
Based on above information test chemical was considered as non-toxic to microorganism.
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