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EC number: 602-891-7 | CAS number: 122731-59-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar 1993 to Dec 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- rac-5-Amino-N-(2,3-diacetoxypropyl)-2,4,6-triiodoisophthalamic acid
- EC Number:
- 602-891-7
- Cas Number:
- 122731-59-3
- Molecular formula:
- C15 H15 I3 N2 O7
- IUPAC Name:
- rac-5-Amino-N-(2,3-diacetoxypropyl)-2,4,6-triiodoisophthalamic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
For the stock solution 12.6 g of ZK 91.248 were dissolved in 1000 mL of bidistilled water. This solution was used for the highest concentration. For the test concentrations, 7500, 5000 and 2500 mg/L of the stock solution were diluted 1:2 and 1:4. 80 mL of the respective solution were filled into the respective test vesseL
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- The microorganisms for the pre-culture were taken from a stock up to seven days old, approximately 7 hours before the start of the incubation in the test. They were kept in a pre-culture nutrient solution consisting of 25 mL solution 1.25 mL solution 3 and 50 mL solution 4. 900 mL of sterile dist. water were added. The pre-culture had a turbidity of 0.086 extinction units on the spectrophotometer. This pre-culture was incubated for approximately 7 hours at room temperature. After the incubation period the turbidity of the pre-culture was measured with 0.648 extinction units, and the pre-culture was dissolved to 0.228 (equivalent to approximately TE/F 50) extinction units with the pre-culture medium, ready to be used as inoculum.
2.5 mL nutrient solution 2, 2.5 mL nutrient solution 3, and 5 mL nutrient solution 4, respectively, were added to the 80 mL test substance solutions. Finally, 10 mL of the inoculum were added. Each test concentration and the control without test compound were set up in triplicate. Additionally, one test vessel was used for each test concentration and the control without inoculum in order to analyze the substance-dependent turbidity.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
Test conditions
- Test temperature:
- room temperature
- Nominal and measured concentrations:
- Nominal: control, 2500, 5000, 7500 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
EFFECT PARAMETERS MEASURED
The turbidity of the control was measured at 0 and 16 hours, whereas the turbidity of the test solutions was measured only after 16 hours (photometer Methrom 662, light path of 2 cm). Additionally, measurements of the control and the test concentrations without inoculum were performed after 16 hours. All measurements were made at a wavelength of 436 nm.
The measured extinction was transformed to turbidity units (TE/F) according to DIN 38404, part 2, using a calibration table. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
- Conclusions:
- The EC10 of Tamip diacetate (ZK 91.248) was higher than 10 g/L.
- Executive summary:
The compound was studied in a microbial growth test with the bacterium Pseudomonas putida according to DIN 38.412, L8. The test was performed under GLP. The microorganisms were incubated with the test substance over a period of 16 hours.The EC10 was higher than 10 g/L.
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