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EC number: 485-320-2 | CAS number: 221667-31-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 - 11 Aug 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EG L 133 Part C. 11 (1988)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Type of application: direct weighing
- Controls: Two controls without test item are included in the test design, one at the start and the other at the end of the test series. One physico-chemical oxygen consumption control (10000 mg/L) was performed.
- Test concentration separation factor: 10
- Other relevant information: The test item has been added to about 130 mL deionized water and stirred overnight before testing (equilibration phase). - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Culture type: Mixed population of aquatic microorganisms (activated sludge)
- Name and location of sewage treatment plant where inoculum was collected: Municipal STP Cologne-Stammheim (aeration tank of a domestic sewage treatment plant).
- Pretreatment: Aeration of the activated sludge, daily fed with synthetic medium
- pH of the suspension before application: 7.2 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.1 - 22.6 °C
- pH:
- 6.8 - 7.7
- Nominal and measured concentrations:
- Nominal test concentrations (mg/L) : 0 (Control), 100, 1000, 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Aeration: yes, permanent aeration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration: 840.0 mg/L suspended solids
- Nutrients provided for bacteria: A synthetic sewage feed was made by dissolving the following amounts of substance in 1 litre of water.
16.0 g peptone
11.0 g meat extract
3.0 g urea
0.7 g NaCl
0.4 g CaCl2 • 2H2O
0.2 g MgSO4 • 7H2O
2.8 g K2HPO4
EFFECT PARAMETERS MEASURED: respiration rate - Reference substance (positive control):
- yes
- Remarks:
- 3.5- Dichlorophenol; Test concentration: 5, 10 and 20 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 2 in "Any other information on results incl. tables".
Reference
AE 0001789 showed 13.0 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
The EC50 is higher than 10000 mg/L. The effect value related to nominal concentration, since no analytical monitoring was performed.
Table 1: Respiratory rate of the test item and the controls
Test concentration [mg/1] |
Respiratory rate test item [mg/1 • h] |
Phys.-chem. O2 consumption [mg/1 • h] |
Respiratory rate - phys.-chem. O2 consumption [mg/1 • h] |
Inhibition [%] |
100 |
39.0 |
*0.0 |
39.0 |
0.0 |
1000 |
39.0 |
*0.0 |
39.0 |
0.0 |
10000 |
30.0 |
0.0 |
30.0 |
13.0 |
Control, mean |
34.5 |
|
|
|
Control 1 |
36.0 |
|
|
|
Control 2 |
33.0 |
|
|
|
Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
* The physico-chemical oxygen consumption has been determined at 10000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Table 2: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
The mean oxygen uptake rate of the blank controls was 41.1 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
The coefficient of variation of oxygen uptake rate in control replicates was 6.1% at the end of definitive test. |
yes |
Description of key information
EC50 (3 h) > 10000 mg/L (nominal, activated sludge, OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The respiration inhibition of the test item was tested in a respiration inhibition test using activated sludge fed with synthetic sewage (OECD 209, M-276100-01-1). Only a moderate inhibition of 13% compared to the control was observed at the highest test item concentration of nominal 10000 mg/L. Thus, a 3 h EC50 for the test substance was reported as >10000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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