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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: test sample form each vessel was taken form top mid and lower surfaces for analysis
- Sample storage conditions before analysis: chemical was immediately analysed after sampling - Vehicle:
- no
- Details on test solutions:
- Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: The stock solution was prepared by dissolving 100 mg of test chemical in 100 ml of M7 medium to get the final concentration of 1000 mg/L with continous stirring of 21hours followed by filtration and then analytically detected. The final solubility value obtained after analytical detection was 1000.92 mg/L.
- Controls: M7 Medium (Control),
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s)
including control(s)): Not applicable - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Eggs of Daphnia magna were obtained from MicroBio tests Kleimoer 15B-9030 MARI AKERKE (GENT) BELGIUM. Later test system was sub-cultured in the test facility and same was used for
the study
- Age at study initiation (mean and range, SD): <24 h
- Age of parental stock (mean and range, SD): > 3 weeks
- Feeding during test : No
Others: A population of parthenogenetic females of synchronized age structure has been maintained for more than 2 years in the test facility under constant temperature conditions (18 to 22°C) at a 16 :
8 hour light-dark photoperiod (illumination: < 1000 lux). The culture media (M7 medium') was partly renewed twice a week. During the maintenance of test organism, test daphnids were fed with unicel
lular green algae (Selenestrum capricornutum).
ACCLIMATION
- Acclimation period: 48 hours prior test
- Acclimation conditions (same as test or not): Yes (healthy gravid females were acclimatized)
- Type and amount of food: Food was provided during acclimatization.
- Health during acclimation (any mortality observed): healthy no mortality was observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 164 mg CaCO3/L
- Test temperature:
- 19.7°C
- pH:
- 7.4
- Dissolved oxygen:
- 9.0
- Conductivity:
- 0.27 μS/cm
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 25 mL Beakers
- Type (delete if not applicable): open / closed Loosely covered with glass lid to reduce the loss of
water due to evaporation and to avoid the entry of dust into the solutions.
- Material, size, headspace, fill volume: Glass, volume 20 mL
- Volume of solution: 20 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): Not applicable
- Biomass loading rate: 5 daphnids/20 mL test medium
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light- 8 h dark
- Light intensity: 1337-1346 Lux
RANGE-FINDING STUDY
Based on the available data on the test chemical range finding test was not conducted directly main
study was intaited to draw the conclusion on test substance toxicity. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Details on results
- Mortality of control: No mortality in control
- Other adverse effects control: None
- Immobilisation of control: No immobilisation
- Abnormal responses: None in control - Results with reference substance (positive control):
- - Results with reference substance valid? Yes valid
EC50: 0.650 mg/L
Lower limit = 0.631 mg/L - Reported statistics and error estimates:
- Not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test concentrations were measured and found to remain within ±20% only in the 0th day of nominal. Hence, the EC50 > 100 mg/L was expressed based on nominal concentrations.
- Executive summary:
This study was designed (as per OECD 202, adopted in 2004) to assess the acute toxicity of test chemical following exposure of daphnids up to 48h by static method. The brood daphnids were acclimatized 48 hours prior to the test item exposure. Less than 24 h old daphnids were collected from the acclimatized gravid females and exposed to the test item. After exposure on day 0, daphnids were observed for immobilization at 24 and 48 h. M7 medium was used as control, and the same was used for test item formulation . 25 mL glass beakers having a solution volume of 20 mL were used in the test. . Main study was conducted using 0 (control), and 100 mg/L concentrations (limit test). 4 replicates/concentration having 5 daphnids/replicate was used for the main study. Normal behavioural response and no immobilization (0% mortality) were observed up to 48 h followed by control groups , there was no immobilisation was observed in both controla and exposed group. UV-Visible spectrophotometer method was used for active ingredient analysis along with stability of the test item in the test medium. Test item was found to be stable in the test medium. The active ingredient content results were considered acceptable (80-120%) as during main study 0 h and 48 h avg. recovery for 100 mg/L of exposure, which were found in acceptable range between 99.37 at zero hours and 98.29 at 48 hours . Hence the results were based on nominal concentration since the deviation in the initial measured concentration didn’t exceed 20%. Environmental parameters such as pH (7.4 -7.7), temperature (19.7 -21.9 °C), dissolve oxygen (8.8 -6.9 mg/L), hardness (164 mg CaCO3/L), conductivity (0.27 μS/cm), photoperiod (16 h light- 8 h dark) was maintained in acceptable range throughout the test. Feed was not provided during the test. The 48-h EC50of test chemical to, Daphnia magna was reported to be >100mg/L. The 48-h EC50of reference item(Potassium dichromate) to daphnid, Daphnia magna(found to be in acceptable range) is 0.640 mg/L. Hence, the results of the test with reference item establish the acceptability of the test system response, test procedures followed, and results obtained with test item. Hence, as per CLP classification category the test chemical can be categorized as Not classified category.
Reference
Sr. No |
Concentrations mg/L |
Wavelength (nm) |
Absorbance |
Temperature (°C) |
1 |
blank |
00 | 00 | 25 |
2 |
5.0 | 199 | 0.02 | 25 |
3 |
10.0 | 199 | 0.04 | 25 |
4 |
20.0 | 199 | 0.08 | 25 |
5 |
40.0 | 199 | 0.17 | 25 |
6 |
60.0 | 199 | 0.28 | 25 |
7 |
80.0 | 199 | 0.38 | 25 |
8 |
100.0 | 199 | 0.49 | 25 |
9 |
120.0 | 199 | 0.60 | 25 |
10 |
140.0 | 199 | 0.69 | 25 |
11 |
160.0 | 199 | 0.78 | 25 |
12 |
180.0 | 199 | 0.87 | 25 |
13 |
200.0 | 199 | 0.95 | 25 |
0 Hour |
0 Hour |
48 Hours |
48 hours |
||
Sr. No |
Concentration’s(mg/L) |
Absorbance (mean) |
Analytical concentrations |
Absorbance (mean) |
Analytical concentrations |
1 | control | 00 | 00 | 00 | 00 |
2 | 100 | 0.48 | 99.37 | 0.47 | 98.29 |
Nominal conc. (mg/l) | Number of Daphnids/replicate | Immobility | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
0h | 24h | 48h | Cumulative Immobilization | Percent Inhibition | |||||||||||
control | 5 | R1=0 | R2=0 | R3=0 | R4=0 | R1=0 | R2=0 | R3=0 | R4=0 | R1= | R2= | R3= | R4= | - | - |
100 | 5 | R1=0 | R2=0 | R3=0 | R4=0 | R1=0 | R2=0 | R3=0 | R4=0 | R1= | R2= | R3= | R4= | - | - |
Any abnormal behavior(if any) | Actively moving | Actively moving | Actively moving |
Nominal conc. (mg/l) | Temperature(° C) | DO(mg/l) | pH | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
control | R1=19.7 | R2=19.7 | R3=19.7 | R4=19.7 | R1=8.8 | R2=8.8 | R3=8.8 | R4=8.8 | R1=7.4 | R2=7.4 | R3=7.4 | R4=7.4 |
100 | R1=19.7 | R2=19.7 | R3=19.7 | R4=19.7 | R1=9.0 | R2=9.0 | R3=9.0 | R4=9.0 | R1=8.1 | R2=8.1 | R3=8.1 | R4=8.1 |
Nominal conc. (mg/l) | Temperature(° C) | DO(mg/l) | pH | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
control | R1=19.4 | R2=19.4 | R3=19.4 | R4=19.4 | R1=8.9 | R2=8.9 | R3=8.9 | R4=8.9 | R1=7.6 | R2=7.6 | R3=7.6 | R4=7.6 |
100 | R1=19.4 | R2=19.4 | R3=19.4 | R4=19.4 | R1=8.7 | R2=8.7 | R3=8.7 | R4=8.7 | R1=8.2 | R2=8.2 | R3=8.2 | R4=8.2 |
Nominal conc. (mg/l) | Temperature(° C) | DO(mg/l) | pH | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
control | R1=21.9 | R2=21.9 | R3=21.9 | R4=21.9 | R1=6.9 | R2=6.9 | R3=6.9 | R4=6.9 | R1=87.7 | R2=7.7 | R3=7.7 | R4=7.7 |
100 | R1=21.9 | R2=21.9 | R3=21.9 | R4=21.9 | R1=6.5 | R2=6.5 | R3=6.5 | R4=6.5 | R1=8.2 | R2=8.2 | R3=8.2 | R4=8.2 |
Description of key information
This study was designed (as per OECD 202, adopted in 2004) to assess the acute toxicity of test chemical following exposure of daphnids up to 48h by static method. The brood daphnids were acclimatized 48 hours prior to the test item exposure. Less than 24 h old daphnids were collected from the acclimatized gravid females and exposed to the test item. After exposure on day 0, daphnids were observed for immobilization at 24 and 48 h. M7 medium was used as control, and the same was used for test item formulation . 25 mL glass beakers having a solution volume of 20 mL were used in the test. . Main study was conducted using 0 (control), and 100 mg/L concentrations (limit test). 4 replicates/concentration having 5 daphnids/replicate was used for the main study. Normal behavioural response and no immobilization (0% mortality) were observed up to 48 h followed by control groups , there was no immobilisation was observed in both controla and exposed group. UV-Visible spectrophotometer method was used for active ingredient analysis along with stability of the test item in the test medium. Test item was found to be stable in the test medium. The active ingredient content results were considered acceptable (80-120%) as during main study 0 h and 48 h avg. recovery for 100 mg/L of exposure, which were found in acceptable range between 99.37 at zero hours and 98.29 at 48 hours . Hence the results were based on nominal concentration since the deviation in the initial measured concentration didn’t exceed 20%. Environmental parameters such as pH (7.4 -7.7), temperature (19.7 -21.9 °C), dissolve oxygen (8.8 -6.9 mg/L), hardness (164 mg CaCO3/L), conductivity (0.27 μS/cm), photoperiod (16 h light- 8 h dark) was maintained in acceptable range throughout the test. Feed was not provided during the test. The 48-h EC50of test chemical to, Daphnia magna was reported to be >100mg/L. The 48-h EC50of reference item(Potassium dichromate) to daphnid, Daphnia magna(found to be in acceptable range) is 0.640 mg/L. Hence, the results of the test with reference item establish the acceptability of the test system response, test procedures followed, and results obtained with test item. Hence, as per CLP classification category the test chemical can be categorized as Not classified category.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
This study was designed (as per OECD 202, adopted in 2004) to assess the acute toxicity of test chemical following exposure of daphnids up to 48h by static method. The brood daphnids were acclimatized 48 hours prior to the test item exposure. Less than 24 h old daphnids were collected from the acclimatized gravid females and exposed to the test item. After exposure on day 0, daphnids were observed for immobilization at 24 and 48 h. M7 medium was used as control, and the same was used for test item formulation . 25 mL glass beakers having a solution volume of 20 mL were used in the test. . Main study was conducted using 0 (control), and 100 mg/L concentrations (limit test). 4 replicates/concentration having 5 daphnids/replicate was used for the main study. Normal behavioural response and no immobilization (0% mortality) were observed up to 48 h followed by control groups , there was no immobilisation was observed in both controla and exposed group. UV-Visible spectrophotometer method was used for active ingredient analysis along with stability of the test item in the test medium. Test item was found to be stable in the test medium. The active ingredient content results were considered acceptable (80-120%) as during main study 0 h and 48 h avg. recovery for 100 mg/L of exposure, which were found in acceptable range between 99.37 at zero hours and 98.29 at 48 hours . Hence the results were based on nominal concentration since the deviation in the initial measured concentration didn’t exceed 20%. Environmental parameters such as pH (7.4 -7.7), temperature (19.7 -21.9 °C), dissolve oxygen (8.8 -6.9 mg/L), hardness (164 mg CaCO3/L), conductivity (0.27 μS/cm), photoperiod (16 h light- 8 h dark) was maintained in acceptable range throughout the test. Feed was not provided during the test. The 48-h EC50of test chemical to, Daphnia magna was reported to be >100mg/L. The 48-h EC50of reference item(Potassium dichromate) to daphnid, Daphnia magna(found to be in acceptable range) is 0.640 mg/L. Hence, the results of the test with reference item establish the acceptability of the test system response, test procedures followed, and results obtained with test item. Hence, as per CLP classification category the test chemical can be categorized as Not classified category.
To evaluate the influence of the test item on the mobility and survival of Daphnia magna an Acute Immobilisation Test according to OECD TG 202, was carried out under static conditions and in compliance with the GLP principles. Five animals in four replicates were exposed for 48 hours to nominal test item concentrations of 6.25, 12.5, 25, 50 and 100 mg/L. In addition, a blank control including the test medium but without the test item was performed. The test item concentrations were analytically verified by using HPLC-UV to be 6.609, 12.740, 26.350, 51.560 and 103.228 mg/L after 48 hours. The test item concentration was thus 97 – 101 % of the nominal at the end of the experimental phase (at the start of the test: 102 –106 % of the nominal). Therefore, all biological results are based on nominal concentrations.The suitability of the test system was confirmed by a positive control with the reference substance potassium dichromate (24h EC50 = 1.12 mg/L). All validity criteria of the test guidelines were fulfilled. The mobility of the test animals was recorded after 24 and 48 hours of exposure. As a result, the 48-hour EC50 of the test item was determined to be 94.3 mg/L in nominal (95 % conf. limits: 77.3 – 133.3 calculated). The 48-hour EC10 was found to be 56.1 mg/L in nominal ( 95 % conf. limits: 28.1 – 70.0 mg/L). Based on the outcomes the test chemical can be classified into aquatic chronic category 3 as per CLP classification criteria.
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