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EC number: 210-719-3 | CAS number: 622-08-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: Batch number 170808-29
- Expiration date of the lot/batch: 28/Aug/2019
- Purity test date: 99.71 % w/w - Analytical monitoring:
- yes
- Details on sampling:
- At intervals of 0, 24, 48 and 72 hours of the test, aliquots of each one of the test solutions were taken from the flasks to determine the test substance
concentration - Vehicle:
- yes
- Details on test solutions:
- The primary stock solution used in the range-finding test was prepared by dissolving 0.0250 g of the test substance in the volume was completed to 250 mL with OECD medium, resulting in a concentration of 100 mg.1. 1. The stock solution of 10 mg.L-1 was prepared from dilution of the primary stock solution.
The stock solution used in the definitive test was prepared by dissolving 0.100 g of the test substance in the volume was completed to 1000 mL with OECD medium, resulting in a concentration of 100 mg.L-1.
From these initial stock solutions, a series of dilutions was prepared in order to obtain the desired tested concentrations - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The unicellular green alga species Pseudokirchneriella subcapitata used is part of the Chloroficeae group, Chlorococcales order.
The test system was obtained from Culture Collection of Algae at Goettingen University - SAG 61.81 on October, 08th, 2013and is recommended by the
Guideline OECD 201 (2011).
The stock culture was prepared and maintained under lab-controlled conditions in solid medium with protease peptone.
The inoculated solid medium was kept inside a culture room, with controlled temperature of 21 to 24°C and luminosity of 2100 ± 10% lux. After algae cultures
reached an ideal growth (about one week), they were maintained at temperature of 2 to 6°C and luminosity of 100 to 200 lux in order to slow down the algae
growth. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- 22.0 to 22.3°C
- pH:
- 7.2 to 8.12
- Nominal and measured concentrations:
- Nominal concentration (mg.L-1)
Control
1 0.
3.2
10
32
100
Mean of effective concentration of test substance (mg.L-1)0.9026
2.6836
8.1396
26.9469
81.9080 - Details on test conditions:
- A range-finding test was carried out using the following concentrations of test substance: control; 0.1; 1; 10 and 100 mg.L-1 of test substance, with two replicate per concentration. The algae inoculum was transferred aseptically to each test flask, with calculated volume to yield an initial cell concentration of 5 x 103
After 72 hours, samples of each test flask were taken in order to count the cells and to determine the concentrations, using a Neubauer chamber and a
microscope, that caused algal growth reduction.
Based on the results obtained in the range-finding test, the definitive test was carried out with the following concentrations of test substance: control;
1; 3.2; 10; 32 and 100 mg.L-1.
The test was performed with six replicates per each concentration and control, therefore tree replicate was prepared for determinations
of pH and chemical analysis. The algae inoculum was transferred aseptically to each test flask, with calculated volume to yield an initial cell concentration of 5 x 103 cells.mL-1.
All test flasks were randomly positioned in an incubating chamber with controlled temperature and kept under continuous shaking and lighting conditions.
Every day the flasks were relocated to different places inside the incubating chamber.
The definitive test was carried out under the conditions described below:
Temperature: 22.0 to 22.3°C
Light: 5991 lux (average)
Photoperiod: continuous light
Shaker velocity: 130.8 rpm (average)
Test duration: 72 hours
Temperature was measured daily using a digital thermometer. The pH was measured at the beginning of the test and at 72 hours .
One aliquot was taken from each test flask in order to determine the algal growth over the period of 24, 48 and 72 hours after the start of the test. A Neubauer
chamber and a microscope were used to determine the cell number (cells.mL-1).
A test with the reference substance, potassium dichromate, was also carried out in order to verify the sensitivity of the test system.
The procedure for this test was the same as for the definitive test described above, with initial cell concentration of 5 x 103 cells.mL-1 for each concentration and using nominal concentrations of 0 (control); 0.18; 0.32; 0.56; 1.00 and 1.80 mg/L and two replicates for each test concentrations. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Remarks:
- because the highest tested concentration of 100 mg.L1, caused only 16% inhibition of growth rate.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 3.2 mg/L
- Basis for effect:
- growth rate
- Details on results:
- The 72-hours EC50 value of test substance, obtained in this study with Pseudokirchneriella subcapitata, was 23.42 mg.L-1 (95% confidence limits = 13.78 to 39.79 mg.L-1) and the the 72-hours EC50 value was not determined, because the highest tested concentration of 100 mg.L-1, caused only 16% inhibition of growth rate.
The highest test substance concentration which no observed significant adverse effect (NOEC) on algal growth was 1 mg.L-1 and the lowest test substance concentration at which was observed significant reducing effect on growth (LOEC) was 3.2 mg/L. - Results with reference substance (positive control):
- The 72-hours EC50 value of the reference substance potassium dichromate was 0.64 mg/L (confidence limits at 95% = 0.59 to 0.70 mg1-1), demonstrating that the toxic sensitivity of the algae was within the accepted range (ISO, 1989), which report toxicity ranged between 0.60 and 1.03 mg.L-1.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hours EyC50 value for alga Pseudokirchneriella subcapitata exposed to 2-(Benzyloxy) ethanol was 23.42 mg.L-1 (95% confidence limits = 13.78 to 39.79 mg.L-1) and the the 72-hours EC50 value was not determined, because the highest tested concentration of 100 mg.L1, caused only 16% inhibition of growth rate. The highest test substance concentration which no observed significant adverse effect (NOEC) on algal growth was 1 mg.L-1 and the lowest test substance
concentration at which was observed significant reducing effect on growth (LOEC) was 3.2 mg.L-1.
Reference
Description of key information
The 72-hours EyC50 value for alga Pseudokirchneriella subcapitata exposed to 2-(Benzyloxy) ethanol was 23.42 mg.L-1 (95% confidence limits = 13.78 to 39.79 mg.L-1) and the the 72-hours EC50 value was not determined, because the highest tested concentration of 100 mg.L1, caused only 16% inhibition of growth rate. The highest test substance concentration which no observed significant adverse effect (NOEC) on algal growth was 1 mg.L-1 and the lowest test substance
concentration at which was observed significant reducing effect on growth (LOEC) was 3.2 mg.L-1.
Key value for chemical safety assessment
Additional information
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