Reaction mass of 3-[(diphenoxyphosphoryl)oxy]phenyl triphenyl 1,3-phenylene bis(phosphate) and tetraphenyl 1,3-phenylene bis(phosphate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08/09/1988 - 14/02/1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to international guideline (OECD guideline 402) under GLP. Chemical identity of the test substance is not reported.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate

EC Number:

701-337-2

Cas Number:

not available

Molecular formula:

C30H24O8P2

IUPAC Name:

3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate

Details on test material:

- Name of test material (as cited in study report): CR 733-S
- Physical state: Liquid
- Storage condition of test material: At ambient temperature, in the dark, in a dry place

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River wige GmbH, Sulzfeld, FRG
- Age at study initiation: 9 weeks
- Fasting period before study: No
- Housing: polycarbonate cages containing purified sawdust (Woody Clean supplied by Broekman Institute, Someren, The Netherlands) as bedding material
- Diet (e.g. ad libitum): standard pelleted laboratory animal diet (RMH-B, pellet diameter 10 mm, Hope Farms, Woerden, The Netherlands), ad libitum
- Water (e.g. ad libitum): tap-water, ad libitum
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-20
- Humidity (%): 60-75
- Photoperiod (hrs dark / hrs light): 12/12


IN-LIFE DATES: From: 08/09/1988 To: 22/09/1988

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Type of wrap if used: surgical gauze, fixed with aluminium foil, flexible bandage and adhesive tape


REMOVAL OF TEST SUBSTANCE
- Washing (if done): Test substance gently removed with tissues, moistened with tap water
- Time after start of exposure: 24 hours


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.5 ml/kg bodyweight:
- Constant volume or concentration used: yes

Duration of exposure:

24 hours

Doses:

2000 mg/kg bodyweight

No. of animals per sex per dose:

5 males and 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing: weekly; clinical observations: daily (multiple times on day 0); observation of treated area on days 4, 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, necropsy

Statistics:

No data

Results and discussion

Preliminary study:

Not relevant

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortality occured during the 14-day observation period.

Clinical signs:

other: Signs of toxicity included lethargy and blood nose encrustation on days 0 and 1, respectively. The treated skin surface of animals showed no irritation.

Gross pathology:

Macroscopic examination at necropsy of all animals at the end of the study did not reveal any changes that were considered to have arisen as a result of treatment.

Other findings:

No data

Any other information on results incl. tables

Table showing the mortality and clinical signs of rats in this study.

	Day 0			Day
	0:20	2:15	4:50	1	2	3	4	5	6	7	8	9	10	11	12	13	14
No abnormalities	10	10	0	9	10	10	10	10	10	10	10	10	10	10	10	10	10
Lethargy	0	0	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bloody nose encrustation	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0
Deaths	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute dermal toxicity (LD50) of CR 733-S towards rats is > 2000 mg/kg bodyweight.

Executive summary:

The acute dermal toxicity of CR 733 -S was determined according to OECD guideline 402 under GLP. Five male and 5 female rats were exposed to 2000 mg/kg bodyweight of the test substance for 24 hours and observed for 14 days. The LD50 was found to be > 2000 mg/kg bodyweight for both males and females.