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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From February 8,1990 to February 21,1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant with international guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Provenienza:Hoechst wastewater biological treatment plant
- Concentration of sludge:10 g/L, 1020 mg/L of dry weight - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 44 other: mg C/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- other: COD
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature:22°C
- pH:7.3
- pH adjusted: yes with NaOH
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:
STATISTICAL METHODS: - Parameter:
- other: COD
- Value:
- ca. 4
- Sampling time:
- 28 d
- Details on results:
- no data
- Results with reference substance:
- no reference substance
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- After 29 days of treatment the substance was degraded less than 10%. (~4%)
- Executive summary:
Reactive orange 16 was tested by OECD 302B (Zhan-Wellens test). After 29 days of treatment the substance was degraded less than 10%. (~4%)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- other: read across from supporting substance
- Adequacy of study:
- weight of evidence
- Study period:
- Since November 18,1985 to December 17,1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant with international guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EEC-Directive 79/831, Part C: Methods for the Determination of Ecotoxicity,
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge:municipal wastewater treatment plant, F-Sindlingen
- Duration of test (contact time):
- ca. 29 d
- Initial conc.:
- ca. 58 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test vessel: Erlenmeyer flasks 20 ml
- Test temperature :21°C
- pH:7.1
- pH adjusted: no
-Other:
mother solution: 5 g/L in distilled water - Reference substance:
- other: hydroquinone
- Parameter:
- % degradation (DOC removal)
- Value:
- < 10
- Sampling time:
- 29 d
- Results with reference substance:
- hydroquinone
2.0 in deionized water
4.9, was used with NaOH to pH 7.1. DOC: 1275 mg C / l
Inoculum and reaction medium of instruction (No. 1.6.1.2 and 1.6.4) - Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not ready biodegradable
- Conclusions:
- After 29 days of treatment the substance was degraded less than 10%.
- Executive summary:
- The similar substance 1 was tested by OECD 301E screening test. The substance it not ready biodegradable.
Referenceopen allclose all
Biodegradation within 28 days
time | elimination | biodegradation | mean |
3h | 0% | 0% | < 10% |
7 days | 2% | 2% | |
14 days | -8% | -8% | |
21 days | -2% | -2% | |
28 days | 4% | 4% |
sempling time | control DOC mg/L |
test item DOC, mg/L | test item degradation % |
positive control DOC mg/L |
positive control DOC degradation % |
3h | 2.9 | 18.9 | 5.5 | 16.6 | 17 |
ld | 2.1 | 18.2 | 9 | 17.7 | 11.6 |
3 d | 2 | 19 | 5 | 17 | 15 |
7d | 2 | 19 | 5 | 5.4 | 73 |
10 d | 2.1 | 21.7 | 0 | 6.9 | 65.5 |
17 d | 3.7 | 21.2 | 0 | 5.7 | 71,5 |
21 d | 0.9 | 30** | - | 5.5 | 72.5 |
24 d | 1.6 | 22.4 | 0 | 5.8 | 72,5 |
28 d | 2.6 | 21.4 | 0 | 5.4 | 71 |
29 d | 1.9 | 19.1 | 4.5 | 5.4 | 73 |
** outlier
Description of key information
OECD 301E, similar substance 1, Less than 10% in 28 days
OECD302B, COD ca 4% in 28 days
OECD203B enhanced, similar substance 2, not inherently
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The target substance was tested following OECD 301E screening test for ready biodegradation with a final results < 10% indicating that the substance is not readily biodegradable
Similar substance 1 was tested following OECD 302B for inherently biodegradation. At the end of the test period no biodegradation was observed. therefore Reactive Orange 16 is not classified as inherently biodegradable.Also similar substance 2 showed no biodegradation following enhanced inherently biodegradation test. Simulation test on water and sediment as per OECD309 is therefore submitted as to completely assess the biodegradation behaviour of the substance.
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