4-trans-Pentyl-[1,1-bicyclohexyl]-4-on

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-04-24 to 1992-04-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Controls, 0.1; 0.18; 0.32; 0.58 and 1.0 test item mg/L
- Sampling method: At the beginning of the experiments, for each concentration an additional solution (100 mL) was prepared under the same conditions as the test solution for the analytical verification. The bottles were sealed and stored at <4 °C. 20 mL of the samples were sent to the client for analytical verification. At the end of the experiment, the daphnia were removed and 100 mL of each sample were treated analogous to the samples at the beginning of the experiment. Of these samples also 20 mL each were sent to the client for analytical verification.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A series of stock solutions of the test substance were prepared in acetone. By withdrawing 100 μL/L each, the stated nominal concentrations of the test substance were prepared in the test solutions.
- Controls: Yes, vehicle control (100 µL/L acetone) and control containing ony diluton water (without test substance).
- Chemical name of vehicle: acetone
- Concentration of vehicle in test medium: 100 µL/L

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: STRAUS
- Age at study initiation: < 24 hours
- Feeding during test : No

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

70 mg CaCO3/L

Test temperature:

21 °C

pH:

7.5 (at the start and end of the test)

Dissolved oxygen:

96% (at the start)
89% (at the end)

Nominal and measured concentrations:

Nominal: Controls, 0.1; 0.18; 0.32; 0.58 and 1.0 mg/L
Measured concentrations:

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Material, size, headspace, fill volume: glass, 100 mL, none, 100 mL
- Volume of solution: 100 mL
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard dilution water according to OECD 202

EFFECT PARAMETERS MEASURED: Immobilisation of the Daphnia was recorded after 24h and 48h. Daphnia was considered immobilized if the animals showed no movement within 15 seconds after a gentle touch. If other effects were observed, they were also noted.

VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: No
- Other adverse effects control: No
- Immobilisation of control: No
- Analytical results: The effect concentrations given are based on the nominal concentrations of the test substance. The solutions were analytically checked. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form.

 Table 1: Cumulative immobilization at 24 h and 48 h, Cumulative percentage immobilization in ( )

Concentration [mg/L]	Immobilized daphnids
	24 h	48 h
0	0* (0)	0 (0)
100 µL acetone/L	0* (0)	0 (0)
0.1	0* (0)	0 (0)
0.18	0* (0)	6 (30)
0.32	10* (50)	20* (100)
0.58	20* (100)	20* (100)
1.0	20* (100)	20* (100)

* 20 daphnia were used at the beginning of the experiment.

Validity criteria fulfilled:

yes

Conclusions:

The acute immobilization study with Daphnia magna was performed according to OECD 202 (1992). Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L.

Executive summary:

The effects of the test item to Daphnia magna (STRAUS) were determined in an acute immobilisation test according to OECD 202 (1992). The study was conducted under static conditions over a period of 48 hours with nominal test item concentrations of 0.1, 0.18, 0.32, 0.58 and 1.0 mg/L. Acetone was used as a solvent and a vehicle control containing acetone (100 µL/L) and a control (containing only dilution water) was included in the test. Twenty daphnids were exposed to each concentration level and the controls.

The concentrations of the test item were analytically verified via gas chromatography at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours) in all concentration levels and in the control. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form. Therefore, the effect levels were based on the nominal concentrations of the test item.

Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L.

Description of key information

The acute immobilization study with Daphnia magna was performed according to OECD 202 (1992). Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L (reference 6.1.3-1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.19 mg/L

Additional information

The effects of the test item to Daphnia magna (STRAUS) were determined in an acute immobilisation test according to OECD 202 (1992). The study was conducted under static conditions over a period of 48 hours with nominal test item concentrations of 0.1, 0.18, 0.32, 0.58 and 1.0 mg/L. Acetone was used as a solvent and a vehicle control containing acetone (100 µL/L) and a control (containing only dilution water) was included in the test. Twenty daphnids were exposed to each concentration level and the controls.

The concentrations of the test item were analytically verified via gas chromatography at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours) in all concentration levels and in the control. No test substance was found in any of the test solutions. All measurements were below the detection limit of 0.1 mg/L. This is related to the very low water solubility of the test substance. However, since the observed effects were nevertheless dependent on the concentration, it can be assumed that the test substance has an effect on the immobilization of the daphnia, even in an unresolved form. Therefore, the effect levels were based on the nominal concentrations of the test item.

Under the given conditions the 48h-EC50 value was 0.19 mg/L and the NOEC was 0.1 mg/L (reference 6.1.3-1).