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EC number: 951-779-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2020-11-16 to 2020-11-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Chemical analysis in the test solutions was performed at T=0h and T=72h.
At T=0h, solutions for analysis were taken direcly from starting solutions without algae. At T=72h, measurement swere performed in abiotic replicates.
The algae cells per unit volume were measured during the test at 24, 48 and 72 hours with a particle counter and/or under a microscope with a Malassez counting cell if need be. - Vehicle:
- no
- Details on test solutions:
- According to the results of the preliminary test, we proposed to test five concentrations for this study: 10 – 32 – 102 – 328 and 1050 mg/L(loading rates).
The prepared samples were stirred 24 hours at 20 ± 2°C, darkness. After this step, the samples were filtrated on cellulose acetate filter (porosity: 0.45 µm) after a settling period of 10 minutes.
The solutions obtained were named WAF (Water Accommodated Fraction). - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Freshwater unicellular green alga Pseudokirchneriella subcapitata of the chlorococcal order strain number CCAP 278.4 was used in this study.
The algae cultures are stored in the laboratory on a solid medium (LC/agar Petri plates) and in a liquid medium with regular sub-culturing (every week). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21 to 24 °C
- pH:
- 7.6 - 8
- Nominal and measured concentrations:
- 0, 10, 32, 102, 328, 1050 mg test item /L (loading rates)
- Details on test conditions:
- A growing exponential phase inoculum of the freshwater unicellular alga Pseudokirchneriella subcapitata was added in order to have an initial concentration of 100 000 cells/mL at the start of the test:
- 100 mL of algae cultures were grown in 250 mL erlenmeyer flasks which were placed in a cooling incubator at a temperature in the range of 21 to 24°C controlled at ±2°C and under a constant shaking fixed at 120 rpm and a continuous illumination comprised between 4 440 and 8 880 lux. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Loading rates
- Basis for effect:
- other: yield (migrated information)
- Remarks on result:
- other: CL: [47-136] mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Loading rates
- Basis for effect:
- other: yield (migrated information)
- Remarks on result:
- other: CL: [0-24] mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Loading rates
- Basis for effect:
- growth rate
- Remarks on result:
- other: CL: [21-44] mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 349 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Loading rates
- Basis for effect:
- growth rate
- Remarks on result:
- other: CL: [260-498] mg/L
- Results with reference substance (positive control):
- Results obtained with the reference item potassium dichromate:
The last algal growth inhibition test performed with the reference item K2Cr2O7 (June 02, 2020) gave an ErC50(0-72h = 0.88 mg/L (0.82 – 0.95), indicating that the algae sensitivity is correct (within the accepted interval of 0.65 to 1.73 mg/L fixed by the International Standard NF EN ISO 8692 from Mai 2012 as mentioned in the OECD 201 Guideline) and in accordance with previous data obtained by the laboratory. - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of Total Carbon in solutions containing the test item “TOBACCO ABSOLUTE”, the measured concentration are well maintained within ± 20%t of the initial measured Total Organic Carbon (- 0.4% to +15.5%).
Consequently, the test item “TOBACCO ABSOLUTE” can be considered as stable in the testing conditions after 72 hours of exposure.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL50-(0-72h) = 349 mg/L [260 – 498], value expressed in nominal test item loading rate.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL10-(0-72h) =28 mg/L [21 – 44], value expressed in nominal test item loading rate. - Executive summary:
The aim of this study was to determine the effects of the test item “TOBACCO ABSOLUTE” on the growth of the unicellular green alga Pseudokirchneriella subcapitata, according to the OECD 201 guideline (March 2006, corrected July 2011).
The algae were exposed to Water Accommodated Fractions (WAFs) of the test item, following the “Guidance document on aquatic toxicity testing of difficult substances and mixtures” (OECD No. 23).
There was no deviation and no amendment during this study.
Following the results of preliminary tests (non-GLP), a final test at five nominal test item loading rates was performed, in order to evaluate the significant effect on the test organism. The effects on the algal growth were determined by cell counting daily for 72 hours.
The concentration of “TOBACCO ABSOLUTE” in the control and the solutions was checked from WAF algal solutions by Carbon Analyzer during the study at the start (T=0h) and at the end of the test (T=72h).
Test item: TOBACCO ABSOLUTE
Test organisms: Pseudokirchneriella subcapitata
Exposure duration: 72 hours
Test concentrations: Control – 10 – 32 – 102 – 328 and 1050 mg/L
Incubator: Temperature 22.8 °C – 23.2°C ; shaking at 120 rpm; light intensity: 5928 Lux
Measurement of cell growth: Beckman Coulter Z2 and Malassez counting cell
Validity criteria: In the control, the increase of the biomass during 72 hours was determined to be higher than a factor of 16.The coefficients of variation of daily section-by-section specific growth rate and of average specific growth rate were determined respectively to be less than 35% and 7% in the control.
The validity criteria were successfully fulfilled.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL50-(0-72h) = 349 mg/L [260 – 498], value expressed in nominal test item loading rate.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL10-(0-72h) =28 mg/L [21 – 44], value expressed in nominal test item loading rate.
Reference
pH values and observations
Nominal test item loading rate (mg/L) | pH at the start (biotic replicates) | pH after 72 hours (biotic replicates) |
WAF Control | 7.9 | 8.0 |
WAF 10 | 7.9 | 7.7 |
WAF 32 | 7.9 | 7.7 |
WAF 102 | 7.9 | 7.6 |
WAF 328 | 7.9 | 7.7 |
WAF 1050 | 8.0 | 7.8 |
Algal growth inhibition
Nominal test item loading rate (mg/L) | [algae] *104 /mL at 24 hours | [algae] *104 /mL at 48 hours | [algae] *104 /mL at 72 hours | |||||||
Count. | Average | CV | Count. | Average | CV | Count. | Average | CV | ||
WAF Control | a | 2,6 | 2,8 | 12,1 | 15,6 | 16,1 | 7,6 | 122,7 | 128,8 | 4,6 |
b | 3,2 | 15,8 | 121,7 | |||||||
c | 3,2 | 14,7 | 128,7 | |||||||
d | 2,7 | 17,6 | 137,8 | |||||||
e | 2,4 | 15,3 | 129,8 | |||||||
f | 2,6 | 17,6 | 131,8 | |||||||
WAF 10 | a | 2,7 | 2,5 | 6,0 | 17,8 | 17,0 | 4,2 | 131,7 | 126,2 | 4,2 |
b | 2,4 | 16,8 | 125,8 | |||||||
c | 2,5 | 16,4 | 121,1 | |||||||
WAF 32 | a | 2,6 | 2,5 | 4,6 | 17,9 | 17,8 | 6,7 | 129,6 | 131,5 | 5,3 |
b | 2,6 | 19,0 | 139,2 | |||||||
c | 2,4 | 16,6 | 125,6 | |||||||
WAF 102 | a | 2,6 | 2,6 | 2,2 | 11,9 | 11,6 | 2,3 | 31,6 | 25,5 | 20,9 |
b | 2,6 | 11,5 | 22,8 | |||||||
c | 2,5 | 11,4 | 22,0 | |||||||
WAF 328 | a | 2,4 | 2,4 | 2,4 | 3,1 | 3,0 | 3,9 | 11,5 | 11,4 | 6,1 |
b | 2,4 | 2,9 | 10,7 | |||||||
c | 2,5 | 2,9 | 12,1 | |||||||
WAF 1050 | a | 2,3 | 2,2 | 2,6 | 2,4 | 2,4 | 0,0 | 5,9 | 5,7 | 4,6 |
b | 2,2 | 2,4 | 5,4 | |||||||
c | 2,2 | 2,4 | 5,8 |
Description of key information
The aim of this study was to determine the effects of the test item “TOBACCO ABSOLUTE” on the growth of the unicellular green alga Pseudokirchneriella subcapitata, according to the OECD 201 guideline (March 2006, corrected July 2011).
The algae were exposed to Water Accommodated Fractions (WAFs) of the test item, following the “Guidance document on aquatic toxicity testing of difficult substances and mixtures” (OECD No. 23).
There was no deviation and no amendment during this study.
Following the results of preliminary tests (non-GLP), a final test at five nominal test item loading rates was performed, in order to evaluate the significant effect on the test organism. The effects on the algal growth were determined by cell counting daily for 72 hours.
The concentration of “TOBACCO ABSOLUTE” in the control and the solutions was checked from WAF algal solutions by Carbon Analyzer during the study at the start (T=0h) and at the end of the test (T=72h).
Test item: TOBACCO ABSOLUTE
Test organisms: Pseudokirchneriella subcapitata
Exposure duration: 72 hours
Test concentrations: Control – 10 – 32 – 102 – 328 and 1050 mg/L
Incubator: Temperature 22.8 °C – 23.2°C ; shaking at 120 rpm; light intensity: 5928 Lux
Measurement of cell growth: Beckman Coulter Z2 and Malassez counting cell
Validity criteria: In the control, the increase of the biomass during 72 hours was determined to be higher than a factor of 16.The coefficients of variation of daily section-by-section specific growth rate and of average specific growth rate were determined respectively to be less than 35% and 7% in the control.
The validity criteria were successfully fulfilled.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL50-(0-72h) = 349 mg/L [260 – 498], value expressed in nominal test item loading rate.
The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “inhibition of growth rate” indicate an ErL10-(0-72h) =28 mg/L [21 – 44], value expressed in nominal test item loading rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 349 mg/L
- EC10 or NOEC for freshwater algae:
- 28 mg/L
Additional information
An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, is on-going. It should be considered as a key study. The result should be retained as key data.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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