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EC number: 431-540-9 | CAS number: 170573-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 06, 2003 to August 06, 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-system
- Details on sampling:
- Analytical report 798142 (Biedermann, 2003)
One sample was taken from the freshly prepared stock solution and three samples were taken from the freshly prepared test media of each test concentration and the control, at first treatment period (Day 0), at a treatment period in the second week (Day 12) and at a treatment period in the last week (Day 16). To determine the maintenance of the test substance concentrations during the test medium renewal periods (2 or 3 days), two stability control samples (with and without food particles) were taken at the end of two treatment periods (on Days 14 and 19). Immediately after sampling, the test substance was extracted from the water phase of the samples by dichloromethane. These organic test substance solutions were stored until analysis in a refrigerator. Based on a pre-experiment, the test substance was stable under these storage conditions. - Vehicle:
- no
- Details on test solutions:
- Prior to the start of the test, an aliquot of the test substance was carefully melted for ca. 30 min in a water bath at 40°Cto increase fluidity and homogeneity. After that, a stock dispersion of nominal 100 mg/L was freshly prepared by dispersing nominal 100 mg of the test substance homogeneously in 1000 mL test water by intensive stirring for 2 x 10 min and ultrasonic treatment for 2 x 15 min. Immediately after, this stock dispersion was diluted in relation 1:10 with test water. Then, adequate volumes of the intensively stirred diluted stock dispersion were mixed into test water to obtain the desired test concentrations.
The media with treatment as well as the control were renewed on Days 2, 5, 7, 9, 12, 14, 16 and 19. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed with females from a clone of of the species of Daphnia magna Straus, supplied originally by the university of Sheffield, UK.
Number of animals: 15-20 per beaker
Age: <24h
Temperature: 18-20°C
Artificial light-dark cycle of 16 to 8 hours with a 30 min transition period
Feeding during test: algae (Desmodesmus subspicatus) (the carbon content of the algae and fish food suspensions were determined using a Shimadzu TOC 5000 Analyser) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 2.5 mmol/L (250 mg/L) as CaCO3
- Test temperature:
- 18-20°C
- pH:
- 7.6-8.3
- Dissolved oxygen:
- at least 8.3 mg/L
- Nominal and measured concentrations:
- 0.00, 0.01, 0.032, 0.10, 0.32 and 1.00 mg/L (nominal) (based on a first test)
- Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.6 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: reproduction and survival rates
- Remarks on result:
- other: 0.22 mg/L (measured)
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.79 mg/L (measured)
- Details on results:
- - Analytical results:
In test media: 86-99% of nominal 0.32 and 1.0 mg/L (meaning a correct preparation)
Stability control samples. Without food particles: 25-64% of the nominal values and with food particles: 11-41% of the nominal values (a part of the test substance could be absorbed onto the food particles. Since filter feeding test animals as Daphnia may take up the test substance also from ingested food, the decrease of the test substance concentration was not taken into account).
Therefore, the biological results werre based on the concentrations in the test media without food. The mean measured concentrations were 0.22 mg/L (0.32 mg/L nominal) and 0.79 mg/L (1.0 mg/L nominal).
- Biological results:
In the control and all doses tested, the survival rate of the animals at the end of the test was al least 80% or higher. The 20% rate left was not considered significant since it was not dose dependent and tolerated by the test guideline.
The first young offspring released from their parent animals were recorded in the control and at all test doses up to 0.22 mg/L (nominal 0.32 mg/L) on Day 9. At the highest test concentration of 0.79 mg/L (1.0 mg/L nominal), first offspring were recorded on Day 12. The reproduction rates calculated for each individual test animal in the control was 122.1 +/- 18.7 alive offspring per adult at the end of the test. No significant toxic effect of the test substance on this parameter was observed up to and including the test concentration of 0.22 mg/L (nominal 0.32 mg/L). At the concentration of 0.79 mg/L (1.0 mg/L nominal), the mean reproduction rate of surviving daphnids was statistically reduced of about 20.2% compared to the control. The 21d EC50 for the reproduction rate of Daphnia magna was estimated to be 0.6 mg/L.
NB. No abnormalities was observed.
In conclusion, the 21d NOEC was 0.22 mg/L (0.32 mg/L nominal) and the 21d LOEC was 0.79 mg/L (1.0 mg/L).
No remarkable observations were made concerning the appearance of the test media. - Reported statistics and error estimates:
- The NOEC and the LOEC of the reproduction rate were statistically evaluated by testing the mean reproduction rate at the test concentrations for statistically significant differences to the control value by the multiple Williams-test after a one-way analysis of variance (ANOVA). The EC50 of the reproduction rate after 21 d was estimated by linear interpolation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 21 d nominal NOEC of the test substance for reproduction rate of Daphnia magna was estimated to be 0.32 mg/L (0.22 mg/L measured).
- Executive summary:
A long term toxicity study was conducted in Daphnia magna with the test substance, isoC18 MIPA (96% active), according to OECD Guideline 204, in compliance with GLP. Based on a pre-test, daphnids were exposed to a blank control and to test substance concentrations of 0, 0.01, 0.032, 0.10, 0.32 and 1.00 mg/L (nominal) for 21 d, applying a semi-static design. Test substance concentrations were verified analytically with HPLC and MS detection. In the test media, 86-99% of the nominal concentrations were found. The stability of control samples were without food particles: 25-64% of the nominal values and with food particles: 11-41% of the nominal values. A part of the test substance could be absorbed onto the food particles. Since filter feeding test animals such as Daphnia may take up the test substance also from ingested food, the decrease of the test substance concentration was not taken into account. Therefore, the biological results were based on the concentrations in the test media without food. The mean measured concentrations were 0.22 mg/L (0.32 mg/L nominal) and 0.79 mg/L (1.0 mg/L nominal). In the control and all doses tested, the survival rate of the animals at the end of the test was 80% or higher. The first young offspring released from their parent animals were recorded in the control and at all test doses up to 0.22 mg/L (nominal 0.32 mg/L) on Day 9. At the highest test concentration of 0.79 mg/L (1.0 mg/L nominal), first offspring were recorded on Day 12. The reproduction rates calculated for each individual test animal in the control was 122.1 +/- 18.7 alive offspring per adult at the end of the test. No significant toxic effect of the test substance on this parameter was observed up to and including the concentration 0.22 mg/L (nominal 0.32 mg/L). At 0.79 mg/L (1.0 mg/L nominal), the mean reproduction rate of surviving daphnids was statistically reduced about 20.2% compared to the control. Moreover, no abnormalities were observed. Under the study conditions, the nominal 21 d NOEC of the test substance was 0.32 mg/L (0.22 mg/L measured) (Peither, 2003).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.22 mg/L
Additional information
A long term toxicity study was conducted in Daphnia magna with the test substance, isoC18 MIPA (96% active), according to OECD Guideline 204, in compliance with GLP. Based on a pre-test, daphnids were exposed to a blank control and to test substance concentrations of 0, 0.01, 0.032, 0.10, 0.32 and 1.00 mg/L (nominal) for 21 d, applying a semi-static design. Test substance concentrations were verified analytically with HPLC and MS detection. In the test media, 86-99% of the nominal concentrations were found. The stability of control samples were without food particles: 25-64% of the nominal values and with food particles: 11-41% of the nominal values. A part of the test substance could be absorbed onto the food particles. Since filter feeding test animals such as Daphnia may take up the test substance also from ingested food, the decrease of the test substance concentration was not taken into account. Therefore, the biological results were based on the concentrations in the test media without food. The mean measured concentrations were 0.22 mg/L (0.32 mg/L nominal) and 0.79 mg/L (1.0 mg/L nominal). In the control and all doses tested, the survival rate of the animals at the end of the test was 80% or higher. The first young offspring released from their parent animals were recorded in the control and at all test doses up to 0.22 mg/L (nominal 0.32 mg/L) on Day 9. At the highest test concentration of 0.79 mg/L (1.0 mg/L nominal), first offspring were recorded on Day 12. The reproduction rates calculated for each individual test animal in the control was 122.1 +/- 18.7 alive offspring per adult at the end of the test. No significant toxic effect of the test substance on this parameter was observed up to and including the concentration 0.22 mg/L (nominal 0.32 mg/L). At 0.79 mg/L (1.0 mg/L nominal), the mean reproduction rate of surviving daphnids was statistically reduced about 20.2% compared to the control. Moreover, no abnormalities were observed. Under the study conditions, the nominal 21 d NOEC of the test substance was 0.32 mg/L (0.22 mg/L measured) (Peither, 2003).
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