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EC number: 445-090-6 | CAS number: 5614-37-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2013-03-12 to 2013-05-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch No.: 2820246
Purity: ≥99.9% - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- water
- Details on preparation and application of test substrate:
- Since the test substance was soluble in water. An amount of test substance was dissolved in water to prepare 1000 mg/L stock solution, then incorporated into formulated artificial soil with deionised reverse osmosis water. The treatment groups were prepared by test matrix component of test substance stock solution, Silica sand, Deionized water and Basic test substrate. These were mixed by hand to give control, 29, 53, 95, 171, 309, 556 and 1000 mg/kg respectively with a nominal moisture content of approximately 35% of dry weight.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida
- Source: Shanghai Yinquan Agriculture Development Co., Ltd.
- Weight at test initiation (mean and range, SD): wet weight: 300 to 600 mg
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions: same as test - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20.0-21.0 °C
- pH:
- ranged from 6.05 at initiation and 6.11 at termination
- Moisture:
- 37.51% at initiation and 29.66% at termination.
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size):1 litre closed glass vessel
- Amount of soil or substrate: 750 wet weight of substrate
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): Sphagnum peat: 10% w/w; Kaolinite clay: 20% w/w; Silica sand (screened with 0.2 mm sieve): 70% w/w
- Pretreatment of soil: The pH of the artificial soil was adjusted to 6.0±0.5 by the addition of calcium carbonate.
OTHER TEST CONDITIONS
- Photoperiod: continous
- Light intensity: 620 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The gross weight of each beaker of the controls and 1000 mg/kg was recorded on Day 0 before addition to the test or control vessels. The surviving earthworms of each beaker were weighed on Day 14.
Any mortalities were recorded 7 and 14 days after the start of treatment.
The temperature of climate chamber and light intensity were recorded daily hroughout the test.
Soil moisture content and pH were recorded at the start and termination of the test.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 10, 100, 1000 mg/kg
- Results used to determine the conditions for the definitive study: The results showed no mortalities at 10mg/kg and 100mg/kg on the 7th and 14th day and all died at 1000 mg/kg on the 7th day. - Nominal and measured concentrations:
- Nominal: 29, 53, 95, 171, 309, 556 and 1000 mg/kg
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 676 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 756 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Mortalities in control group on 7th and 14th day both were 0%. Mortalities in the treatment groups which were 29 mg/kg and 95 mg/kg of on the 7th and 14th day were 0%; 53 mg/kg group was 2.5% on the 7th day and 12.5% on the 14th day; 171 mg/kg group were 2.5% on the 7th day and 5% on the 14th day; 309 mg/kg group was 0% on the 7th day and 2.5% on the 14th day; 556 mg/kg group was 5% on the 7th day and 17.5% on the 14th day; 1000 mg/kg group was 92.5% on the 7th day and 100% on the 14th day.
There was no statistically significant change in the average body weight of the surviving earthworms in the control group at the beginning and at the end (According to the result of t test, p=0.6333, p>0.05). - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 14d-LC50: 11 mg/kg dry soil weight (95% confidence limits: 10 -12 mg/kg dry soil weight) - Reported statistics and error estimates:
- Data were analyzed by appropriate statistical methods to calculate the LC50with 95% confidence limits using software Toxcalc v5.0.26.
The differences of the average body weight of the surviving earthworms between the control group and the treatment groups were determined oy using the t-Test at the end of the study. The data treatment were carried out with software Toxcalc v5.0.26. - Validity criteria fulfilled:
- yes
- Conclusions:
- Expressed as nominal test substance concentration, the median lethal concentrations of test item on Eisenia fetida are as following:
The 7 days LC50 value was higher than 756 mg/kg dry soil weight.
The 14 days LC50 value was higher than 676 mg/kg dry soil weight. - Executive summary:
The acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test according to OECD 207.
Based on the results of range-finding test, 7 treatment groups and one control group which had four replicates of 10 worms per concentration were included in definitive test. The nominal concentrations of the treatment groups were 29, 53, 95, 171, 309, 556 and 1000 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days exposure.
Mortality of the earthworms in control group was 0, which was not more than 10 percent. So the test was valid.
Under present condition, the 7-Day LC50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations was higher than 756 mg/kg; the 14-Day LC50 was higher than 676 mg/kg.
Reference
Description of key information
Acute study:
The 7-Day LC50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations was higher than 756 mg/kg; the 14-Day LC50 was higher than 676 mg/kg.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 676 mg/kg soil dw
Additional information
The acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test according to OECD 207.
Based on the results of range-finding test, 7 treatment groups and one control group which had four replicates of 10 worms per concentration were included in definitive test. The nominal concentrations of the treatment groups were 29, 53, 95, 171, 309, 556 and 1000 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days exposure.
Mortality of the earthworms in control group was 0, which was not more than 10 percent. So the test was valid.
Under present condition, the 7-Day LC50 for the test substance to earthworms (Eisenia fetida) based on nominal test concentrations was higher than 756 mg/kg; the 14-Day LC50 was higher than 676 mg/kg.
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