Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-303-2 | CAS number: 80-71-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-hydroxy-3-methylcyclopent-2-enone
- EC Number:
- 201-303-2
- EC Name:
- 2-hydroxy-3-methylcyclopent-2-enone
- Cas Number:
- 80-71-7
- Molecular formula:
- C6H8O2
- IUPAC Name:
- 2-hydroxy-3-methylcyclopent-2-en-1-one
Constituent 1
- Specific details on test material used for the study:
- Test item: Methyl Cyclopentenolone
Common name of active ingredient (a.i): Methyl cyclopentenolone
CAS number of a.i: 80-71-7
Molecular weight of a.i.: 112 g.moL-1
Molecular formula of a.i.: C6H8O2
Received on: 12/Dec/2017
Bioagri Laboratórios Ltda. code: AGR-0921/17
Batch number: D270001706002
Physical state: White, crystal powder
Manufacture date: 08/Jun/2017
Expiry date: 07/Jun/2018
Test solutions
- Details on test solutions:
- The primary stock solution used in the range-finding test was prepared by dissolving 10 mg of the test substance in 40 mL of dilution water – M4 and the total volume was completed to 100 mL, resulting in a concentration of 100 mg.L-1. The secondary stock solutions of the 10; 1 and 0,1 mg.L-1 were prepared by dilutions of the primary stock solution.
The stock solution used in the definitive test was prepared by dissolving 50 mg of the test substance in 40 mL of dilution water – M4 and the total volume was completed to 500 mL, resulting in a concentration of 100 mg.L-1.
The test solutions were prepared from dilutions of the stock solutions.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test System:
The test system used for this study is described below, according to Straus (1820):
Kingdom: Animalia
Phylum: Arthropoda
Subphylum: Crustacea
Class: Branchiopoda
Order: Cladocera
Family: Daphniidae
Genus: Daphnia
Species: Daphnia magna
Young Daphnia magna ≤ 24 hours old were obtained from the culture kept at the Ecotoxicology Laboratory, maintenance and cultivation records are archived at BIOAGRI.
Test System Justification:
Daphnia magna is the preferred test species as cited in the Guideline OECD 202 (2004).
As these organisms are very sensitive and easy to breed under laboratory conditions, they are useful for evaluating the acute and chronic toxicity of chemical substances and environmental samples.
Origin of the Test System:
The test system Daphnia magna was obtained from the CAROLINA BIOLOGICAL SUPPLY COMPANY - Burlington, North Carolina (USA) and maintained as a stock culture at BIOAGRI.
Test System Obtainment:
Nearly 24 hours before the Acute Toxicity Test started, Daphnia with eggs were isolated in crystallizing dishes and fed with algae suspension. In the following morning, the newly born Daphnia, aged ≤ 24 hours, were separated from the adults and used in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 174.24 to 185.2 mg.L-1 CaCO3
- Test temperature:
- 20.1 to 20.2°C
- pH:
- 7.7 to 7.8
- Dissolved oxygen:
- 8.0 to 8.1 mg.L-1
- Nominal and measured concentrations:
- In the range-finding test the exposure conditions:
Nominal used concentrations: control; 0.01; 0.1; 1; 10 and 100 mg.L-1;
In the definitive test the exposure condition:
Nominal used concentrations: control; 6.5; 13; 25; 50 and 100 mg.L-1; - Details on test conditions:
In the range-finding test the exposure conditions were:
a) Total number of organisms per each tested concentration or control: 10;
b) Number of replicates per each tested concentration and control: 2;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 0.01; 0.1; 1; 10 and 100 mg.L-1;
g) Temperature of the test solutions: 20.0 to 20.3°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks.
In the definitive test the exposure conditions were:
a) Total number of organisms per each tested concentration or control: 20;
b) Number of replicates per each tested concentration and control: 4;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 6.5; 13; 25; 50 and 100 mg.L-1;
g) Temperature of the test solutions: 20.2 to 20.3°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks;
j) Analytical determination: content dissolved oxygen, pH of the test solutions and the test substance concentration were determined at the beginning of the test and after 48 hours.- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 43.74 mg/L
- Remarks on result:
- other: (95% confidence limits = 35.74 to 53.53 mg.L-1)
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 93.89 mg/L
- Remarks on result:
- other: (95% confidence limits was not determined)
- Details on results:
- In the range-finding test, In 48 hours of exposure, the lowest concentration that caused 100% immobilization to the organisms was 100 mg.L-1 and the highest concentration that caused 0% immobilization to the organism was 1 mg.L-1.
The 24 and 48-hours EC50 values of the product Methyl Cyclopentenolone were 93.89 mg.L-1 (95% confidence limits was not determined) and 43.74 mg.L-1 (95% confidence limits = 35.74 to 53.53 mg.L-1), respectively.
In the definitive test the highest tested concentration that did not cause acute effect (immobility) to the organisms after 48 hours was 13 mg.L-1. The lowest concentration that caused 100% immobility was 100 mg.L-1. - Results with reference substance (positive control):
- The 24-hours EC50 value of the reference substance (potassium dichromate) was 1.03 mg.L-1 (95% confidence limits = 0.91 to 1.17 mg.L-1), showing the sensitivity of the test system (ISO 6341 / OECD 202, 2004).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hours EC50 value of Methyl Cyclopentenolone was 43.74 mg.L-1 (95% confidence limits = 35.74 to 53.53 mg.L-1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.