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EC number: 279-013-0 | CAS number: 78948-87-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Fresenius
- Analytical monitoring:
- yes
- Details on sampling:
- initially and after 72 hours
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus CHODAT
- Strain: 86.81
- Source: Sammlung für Algenkulturen, Pflanzenphysiologisches Institut der Unversität Göttingen, Germany
- Pretreatment: 3 days of preculture prior to the test
- Age of inoculum (at test initiation):
- Method of cultivation: Not stated - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24.2 – 25.3 °C
- pH:
- 7.25 – 8.13 (initial)
9.96 – 10.53 (after 72 h) - Nominal and measured concentrations:
- Control, 5, 10, 20, 40, 80 and 100 mg/L (nominal)
It is assumed, that the tests substance is sufficiently stable during the test duration. Therefore the results can be based on the nominal concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass cylinder
- Aeration: prior to the test
- Initial cells density: 6.42 • 104 cells/mL (equals to an extinction E578nm of 0.2)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 7
OTHER TEST CONDITIONS
- Photoperiod: 24 h/d light
- Light intensity and quality: 8000 lux ± 20 % in a wavelength range between 400 and 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Cell multiplication inhibition using a spectrophotometer (extinction measurement at 578 nm);
calculation of growth rate and biomass production
- Sampling: 24, 48 and 72 hours - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 19.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 200 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 99.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The EC50 values are extrapolated and the NOEC values are calculated as EC0 from the dose-response relationship. Based on the percent reductionin growth rate, the NOErC is assumed to be ≥80 mg/L. This value is in good correspendance with the ErC10.
- Results with reference substance (positive control):
- Not performed
- Reported statistics and error estimates:
- ECX and NOEC calculated according to guideline (no further information)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test results indicate that MMPP is acutely of low toxicity towards algae
- Executive summary:
The green alga Scenedesmus subspicatus CHODAT (new scientific name: Desmodesmus subspicatus) was used as the test organism on toxicity tests of MMPP towards algae, and the test was performed according to the guidelines OECD 201 and 92/69/EWG, C.3.
Algae were exposed to seven concentrations of the test substance, ranging from 5 to 100 mg/L in a geometric series and a control group. Initial cell densities were 6.42 • 104 cells/mL. The test was performed at 24 - 25 °C in a fully artificial medium according to OECD guideline 201. Cell densities were assessed at 24, 48 and 72 hours using a spectrophotometer at 578 nm. Physico-chemical water parameters and MMPP concentrations were measured at 0 and 72 hours. Concentrations were analysed by an appropriate HPLC method with UV detection at 412 nm.
MMPP was found to be of low toxicity towards S. subspicatus. The effect data are re-evaluated by the applicant and the resulting numerical results are given below. A clear concentration-response relationship can be established from the study results only for the parameter biomass. However, an NOEC can be estimated from the growth rate results. In addition, the highest concentration tested was set as EC50 (without extrapolation).
The analytical monitoring of the actual test concentrations indicatesthat the tests substance is sufficiently stable during the test duration. Therefore, the results can be based on the nominal concentrations.These results confirm that volatility and adsorption of MMPP from aqueous solutions can be neglected. Although MMPP hydrolyses in aqueous solution, the aqueous solution of MMPP reflects the emission of the active substance (hydrolyses products) to aquatic organisms. In addition, MMPP is regarded as readily biodegradable. The analytical monitoring shows that this property has no influence on the results due to the relatively short test duration.
All validity criteria according to the OECD guideline 201 are met within the study.
Reference
Actual concentrations of test substance
Nominal [mg/L] |
Measured t0[mg/L] |
Measured t72[mg/L] |
100 |
127.61 |
106.89 |
80 |
104.03 |
82.27 |
40 |
45.83 |
46.87 |
Cell concentration data
|
Description of key information
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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