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Diss Factsheets
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EC number: 685-519-6 | CAS number: 93183-15-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 5
- Sampling time:
- 28 d
- Remarks on result:
- other: based on ThOD(NH4)
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Remarks on result:
- other: based on ThOD(NO3)
- Details on results:
- The test item 5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite contains nitrogen; therefore the evaluation of biodegradation has to be based on ThODNH4 and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60%, reached within a 10-day window; the 10-day window starts
when the degradation of the test item reaches 10% degradation.
5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite never reached 10% biodegradation. The mean biodegradation at test end after 28 days was 5% (based on ThODNH4, see Figure 1) and 4% (based on ThODNO3, see Figure 2). - Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 73% after 14 days and to 84 % after 28 days of incubation (based on ThODNH4). The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The mean biodegradation of the test item 5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite at test end after 28 days was 5 % (based on ThODNH4) and 4 % (based on ThODNO3).
- Executive summary:
The biodegradation of 5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite was studied in reconstituted water (pH 7.4 – 7.6) inoculated with activated sludge from a domestic sewage treatment plant (stock suspension of 3.5 g/L on dry matter base and a final sludge concentration in test flasks of 28.7 mg sludge/L). The test item was applied with 103.5 mg a.i./L. The experiment was conducted in accordance with the OECD test guideline 301 F “Ready Biodegradability: Manometric Respirometry Test”, and in compliance with the OECD-GLP standards. The test system consisted of test flasks containing a volume of approximately 500 mL. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB/BODSensor- System, Aqualytic Dortmund, Germany. The test flasks were closed gas-tight by a measuring head. Potassium hydroxide solution (45 %) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
The test item contains nitrogen; therefore the evaluation of biodegradation has to be based on ThODNH4 and ThODNO3. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60 %, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10 % degradation. The test item never reached 10 % biodegradation. The mean biodegradation of the test item at test end after 28 days was 5 % (ThODNH4) and 4 % (ThODNO3). Therefore, 5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite is considered to be not readily biodegradable.
This information is used in a read-across approach in the assessment of the target substance.
For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Reference
Validity Criteria of the Study
- Inoculum Control: The oxygen demand of the inoculum control (medium and inoculum) was 20 mg O2/L and thus not greater than 60 mg O2/L within 28 days as required by the test guideline.
- pH-Value: The pH-value of the test item flasks at the end of the test was 7.4 and therefore within the range of pH 6.0 to 8.5 as required by the test guideline.
- Reference Item: The percentage degradation of the reference item should reach the level for ready biodegradability (> 60 %) within 14 days as required by the test guideline. The reference item sodium benzoate was degraded to more than 60 % after 7 days of incubation.
- Test Item: The difference of duplicate values for the degradation of the test item at the plateau, at the end of the test was less than 20 %. The difference of duplicate values at day 28 differed by 10 % (based on ThODNH4) and 8 % (based on ThODNO3). The validity criterion was fulfilled.
- Toxicity Control: If in a toxicity test, containing both the test substance and a reference compound less than 25 % biodegradation occurred within 14 days (ThODNH4), the test substance can be assumed to be inhibitory. The biodegradation was 32% (based on ThODNH4) and 29 % (based on ThODNO3) at day 14; the test item was not inhibitory.
Description of key information
One study is available for biodegradation in water which can be used for read-across to guanosine, 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxy-N-(2-methyl-1-oxopropyl)-, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]:
The Study by Hammesfahr (2018) was conducted according to GLP principles and followed the OECD test guideline 301 F. The mean degradation rate of 5′-O-[bis(4-methoxyphenyl) phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite in a test according to the OECD method 301 F after 28 days was 5 % (based on ThODNH4) and 4 % (based on ThODNO3). Therefore, the test item is not readily biodegradable.
This information is used in a read-across approach in the assessment of the target substance.
For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.