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EC number: 274-958-5 | CAS number: 70865-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 9, 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted April 4, 1994
- GLP compliance:
- not specified
- Details on test solutions:
- The test article was dissolved in distilled water at a concentration of 500 mg/l.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: aerobic activated sludge from a domestlc waste-water treatment plant (ARA, Sissach/Switzerland).
- Pre-treatment: the sludge was coarsely sieved, centrifugated, the supernatant liquid phase decanted, the solid material resuspended in tap water and again centrifugated. The procedure was repeated a further two times.
- Preparation of inoculum for exposure: an aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (ml) to its dry weight (g) determined. Based on this ratio, a volume of washed sludge suspension corresponding to 4.0 g dry material were made up with Soerenses buffer (pH = 7) to 1 litre. To that mlxture, 50 ml 0ECD reccomended synthetic sewage feed were added. Thereafter, the sludge was incubated overnight at 20 - 25 °C under aeration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- ca 22 °C
- pH:
- 7.8 - 7.9
- Nominal and measured concentrations:
- 10 and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flasks (> 500 ml).
- Aeration: aeration with 0.2 l/minute.
- No. of vessels per concentration: 1 replicate per concentration.
- No. of vessels per control: 2 replicates.
- No. of vessels per reference substance: 1 replicate per concentration.
- Sludge concentration: 200 ml of activated sludge.
DETERMINATION OF OXYGEN CONCENTRATION
- Measurements: oxygen consumption was measured over a period of 10 minutes.
- Conditions: during measurements, the sample was not aerated but continuously stirred on a magnetic stirrer.
VALIDATION OF THE TESTS
The tests are validated by testing a known standard, i.e. 3,5-dichlorophenol. The IC50-value should be in the range of 5 - 30 mg/l.
Furthermore, the variation between the two control samples (start, end) should not be greater than 15 %. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The aeration of test medium containing test article caused the solutions to foam strongly. The foam was coloured. Under these conditions, the respiration rate of the sludge was moderately inhibited (21.0 %) at the highest concentration of test item (i.e. 100 mg/l).
- Results with reference substance (positive control):
- IC5 (3h): 12.9 mg/l
- Validity criteria fulfilled:
- yes
- Remarks:
- variance between the control samples was 12.5 % and the inhibitory effect of reference substance was withing an acceptable range
- Conclusions:
- IC50 (3h) > 100 mg/l (nominal)
- Executive summary:
The inhibltory effect of test item on aerobic waste-water bacteria was investigated in a respiration test, according to the OECD guideline 209. The test article was dissolved in distilled water; the tested concentrations were 10 and 100 mg/l. Two replicates of negative control were prepared and 3,5-dichlorophenol was used as reference substance.
The test article showed moderate inhibition (21.0 %) of the respiration rate up to a concentration of 100 mg/l. The IC50 was determined to be higher than 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l (nominal)
Reference
Influence of test item on oxygen consumption of activated sludge.
Substance | Oxygen consumption (mg O2/l min) | Inhibition (%) | Starting pH |
Control | 0.56 | -- | 7.8 |
Control | 0.63 | -- | 7.9 |
Control mean | 0.595 ± 0.035 (12.5 %) | ||
Test item at 100 mg/l | 0.47 | 21 | 7.8 |
Test item at 10 mg/l | 0.48 | 19.3 | 7.8 |
3,5-dichlorophenol 50 mg/l | 0.04 | 93.3 | 7.8 |
4,5-dichlorophenol 10 mg/l | 0.35 | 41.2 | 7.8 |
5,5-dichlorophenol 3.2 mg/l | 0.56 | 5.9 | 7.8 |
Description of key information
IC50 (3h) > 100 mg/l (nominal)
Key value for chemical safety assessment
Additional information
Two tests are available on the inhibltory effect of Acid Red 057 on aerobic waste-water bacteria. The results obtained in the two different experiments are consistent: it can be concluded that the EC50 (3h) is higher than 100 mg/l; the NOEC/EC10 values are expected to be lower than 10 mg/l.
In the key study , the test article was dissolved in distilled water; the tested concentrations were 10 and 100 mg/l. Two replicates of negative control were prepared and 3,5-dichlorophenol was used as reference substance. The test article showed moderate inhibition (21.0 %) of the respiration rate up to a concentration of 100 mg/l, after the 3 hours exposure period; an inhibition of 19.3 % was recorded at the concentration of 10 mg/l.
In the second experiment available, the substance was firstly assayed in a range-finding test: an inhibition of 13 %, 35 % and 45 % at the tested concentration of 10, 100 and 1000 mg/l, respectively, was recorded. The main test was performed only at the concentration of 10 mg/l and, after the exposure period of 3 hours, the O2 consumption rate in the test vessels containing the test item was not impacted respect to those containing negative control.
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