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EC number: 947-719-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 December 1987 to 18 December 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- verification of test item concentration was not performed
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Purity: 50 % active ingredient
- Lot: 369 - Analytical monitoring:
- no
- Details on sampling:
- - Chemical stability of the test material in well-water was not determined.
- Vehicle:
- no
- Details on test solutions:
- MATERIALS AND METHODS
- The test material, in original form, was placed into 5 L all-glass aquaria and diluted with laboratory well-water to yield the desired nominal exposure concentrations in 4 L final volumes (liquid depth 12 cm).
- Two identical vessels containing only laboratory well-water were designated as the water controls. - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- - Juvenille unsexed fathead minnows.
- Haskell bred stock.
- Mean standard length 1.4 cm (range 1.0 to 1.8 cm).
- Mean wet weight 0.030 g (range 0.008 to 0.067 g).
- Fish were maintained for approximately 67 days, with at least 34 days in a 272 L fibreglass tank (84 cm diameter x approximately 49 cm liquid depth) before being used for the test.
- Temperature was within 3 °C of testing temperature throughout the holding period.
- Dissolved oxygen concentration during acclimatisation was assumed to be at or near saturation because air was continuously bubbled through the water.
- No treatment of fish for diseases was required during the holding period. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
- Hardness:
- - EDTA hardness 91 mg/L as CaCO3 based on water control at zero hours.
- Test temperature:
- 21.9 to 22.2 °C
- pH:
- - Total alkalinity 89 mg/L as CaCO3 based on water control at zero hours.
- pH 6.6 to 7.9 (see Table 2, attached). - Dissolved oxygen:
- 7.8 to 8.8 mg/L (see Table 2, attached).
- Salinity:
- Not applicable.
- Conductivity:
- - 150 μmhos/cm based on water control at zero hours.
- Nominal and measured concentrations:
- - Nominal concentrations of 412, 588, 840, 1200, 1720, 2450, 3500 and 5000 mg/L.
- Details on test conditions:
- - Ten fish were randomly placed in the test vessels (two test vessels per concentration).
- Loading was 0.075 g fish/L.
- Fathead minnows were identified by labelling the culture and test vessels.
- Fish were not fed for approximately 48 hours prior to exposure or during the 96-hour exposure period.
- Test solutions were not aerated.
- Temperature was maintained between 21.9 and 22.2 °C.
- Photoperiod was maintained at 16 hours light and 8 hours dark.
- Mortality counts and observations were made approximately every 24 hours during the test.
- Dissolved oxygen and pH were measured in the water control and in the low test concentrations at the beginning of the test and at approximately 24-hour intervals during the 96-hour exposure period.
- Dissolved oxygen and pH were measured in the medium and high test concentrations at the beginning of the test and within one hour after the test was initiated by which time all the fish had died.
- Total alkalinity, hardness (EDTA) and conductivity were measured at the beginning of the test in the water control. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % confidence interval 1200 to 1400 mg/L
- Details on results:
- - Clinical signs seen in some fish exposed to concentrations of ≥ 1200 mg/L included lying on the bottom, erratic swimming, swimming at the surface, darkening colouration, gasping for air and lethargy.
- Reported statistics and error estimates:
- - The results of LC50 calculations are presented in the table below.
- Concentration data were not altered and the 96-hour LC50 and confidence intervals were calculated by probit analysis (Finney DJ, Probit Analysis, 3rd Ed, Cambridge University Press, 333 pp, 1971). - Sublethal observations / clinical signs:
Table 1 – Results of a 96-hour static acute toxicity test with fathead minnows
Nominal test concentration (mg/L)
Cumulative observed mortality (%)
24 h
48 h
72 h
96 h
A*
B*
A*
B*
A*
B*
A*
B*
5000
100
100
100
100
100
100
100
100
3500
100
100
100
100
100
100
100
100
2450
100
100
100
100
100
100
100
100
1720
100
100
100
100
100
100
100
100
1200
10
40
10
40
20
50
20
50
840
10
0
10
0
10
0
10
0
588
0
0
0
0
0
0
0
0
412
0
0
0
0
0
0
0
0
H20 control
0
0
0
0
0
0
0
0
* Replicate exposure chambers containing ten fish each
LC50 calculations
Time
LC50 (mg/L)
95 % confidence limits (mg/L)
Lower
Upper
24 h
1300
1200
1410
48 h
1300
1200
1410
72 h
1260
1160
1370
96 h
1260
1160
1370
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest test concentration resulting in 0 % mortality was determined to be 588 mg/L and the lowest test concentration resulting in 100 % mortality was determined to be 1720 mg/L. The LC50 (96 h) value was determined to be 1300 mg/L.
- Executive summary:
In a study equivalent or similar to OECD 203 fathead minnows were exposed to eight nominal concentrations of test material plus well-water as the control for 96 hours under static conditions. Ten fish were placed into the test chamber for each treatment group and the control group. Nominal concentrations of test item selected were 412, 588, 840, 1200, 1720, 2450, 3500 and 5000 mg/L. Verification of test item concentration was not performed. Clinical signs observed in fish exposed to ≥ 1200 mg/L included lying on the bottom, erratic swimming, swimming at the surface, darkening colouration, gasping for air and lethargy. The highest test concentration resulting in 0 % mortality was determined to be 588 mg/L and the lowest test concentration resulting in 100 % mortality was determined to be 1720 mg/L. The LC50 (96 h) value was determined to be 1300 mg/L.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 300 mg/L
Additional information
In a study equivalent or similar to OECD 203 fathead minnows were exposed to eight nominal concentrations of test material plus well-water as the control for 96 hours under static conditions. Ten fish were placed into the test chamber for each treatment group and the control group. Nominal concentrations of test item selected were 412, 588, 840, 1200, 1720, 2450, 3500 and 5000 mg/L. Verification of test item concentration was not performed. Clinical signs observed in fish exposed to ≥ 1200 mg/L included lying on the bottom, erratic swimming, swimming at the surface, darkening colouration, gasping for air and lethargy. The highest test concentration resulting in 0 % mortality was determined to be 588 mg/L and the lowest test concentration resulting in 100 % mortality was determined to be 1720 mg/L. The LC50 (96 h) value was determined to be 1300 mg/L.
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