1-bromo-3-chloropropane

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

GLP compliance:

yes (incl. QA statement)

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-bromo-3-chloropropane

EC Number:

203-697-1

EC Name:

1-bromo-3-chloropropane

Cas Number:

109-70-6

Molecular formula:

C3H6BrCl

IUPAC Name:

1-bromo-3-chloropropane

Details on test material:

Batch number : 9207093
Expiry : stable for at least three months
Purity : 99,6%
Appearence : clear colourless liquid
Storage conditions : room temperature in the dark

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Equal number of healthy male and female rats, in the weight range 206 to 246 g, aged 7 to 10 weeks.
A standard laboratory rodent diet (Biosure LAD 1) and drinking water were provided ad libitum.
The mean daily minimum and maximum temperatures of the animal room were 20°C to 22°C respectively and the mean daily relative humidity value was 56% R.H. Air exchange was maintened at 10 to 15 air changes per hour and lighting was controlled by means of a time switch to provide 12 hours of artificial light (07:00-19:00 hours) in each 24 hours period.

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

One day prior to treatment hair was removed from the dorso-lumbar region of each rat with electric clippers exposing an area to approximately 10% of the total body surface.
The test substance was applied by spreading it evenly over the prepared skin. the treated area (approximately 50 mm x 50 mm) was then promptly covered with gauze which was held in place with a non-irritative dressing encircled firmly around the trunk.
A the end of 24-hour exposure period, the dressings were carefully removed and the treated area of skin was washed with warm (30° to 40°C) water and blotted dry with absorbent paper.

Duration of exposure:

24-hour exposure period

Doses:

2,0 g/kg bodyweight

No. of animals per sex per dose:

10 rats (5 males and 5 females)

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 2 000 mg/kg bw

Based on:

test mat.

Mortality:

There were no deaths following a single dermal application of 1-bromo-3-chloropropane at 2,0 g/kg bodyweight.

Clinical signs:

other: Increased locomotor activity and respiratory rate were observed in all rats within two minutes of dosing. These observations were non longer apparent one hour after dosing and there were no further signs. recovery was complete by day 2.

Gross pathology:

No macroscopic abnormalities were observed for animals killed on day 15.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute lethal dermal dose to rats of 1-bromo-3-chloropropane was found greater than 2,0 g/kg bodyweight.
1-bromo-3-chloropropane does not require labelling concerning dermal toxicity in accordance with the CLP regulation.