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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

readily-biodegradable

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradation of the substance was assessed under aerobic static exposure conditions using the Manometric Respirometry Test (OECD 301 F) and EU Method C.4 Part V. The substance was exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant for 28 days and its biodegradability was based on O2 consumption measured continuously measured with a manometric BOD measuring device. Procedure, blank, abiotic sterile and toxicity controls run concurrently and the pH and temperature were checked periodically.

The biodegradability of the substance based on O2 consumption was calculated to be 87.1 % after 28 days as compared to the chemical oxygen demand (COD). The biodegradation reached 63 % at the end of the 10-d window (i.e. within 10 days after attainment of 10 % degradation). The procedure control sodium benzoate reached 80 % biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.

The substance reached the pass level of 60 % for ready biodegradability in the Manometric Respirometry Test within the 10-d window and, therefore, can be termed as readily biodegradable.