Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-572-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Type: mixed population of aquatic microorganisms
- Origin: secondary effluent of a wastewater treatment plant treating predominantly domestic sewage (Wupper area water authority, WWTP Odenthal)
- Date of collection: 2017-07-24
- Pre-treatment: separation of coarse particles by filtration, aeration of the resulting inoculum for 1 day - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Pre-treatment of the test item
- 5.8 mg of the test item were weighed out and added to the test flasks, filled with ca. 300 mL of mineral medium
- inoculum was added
- flasks volume was made up to 1.16 L with mineral medium
- flasks were closed free from air bubbles with glass stoppers.
- initial concentration of test item: 5.0 mg/L (5.8 mg/1.16 L)
Pre-treatment of the reference compound
Reference compound name: sodium benzoate (Acros Organics)
- 500.2 mg sodium benzoate were added to 0.5 litres of deionised water and stirred for 24 hours on a magnetic stirrer
- 3.4 mL of the stock solution were given to the test flasks and filled with ca. 300 mL of mineral medium
- inoculum was added
- flasks volume was made up to 1.16 L with mineral medium
- flasks were closed free from air bubbles with glass stoppers.
- Concentration of stock solution: 1 g/L
- pH value of the stock solution: 7.4
- initial concentration reference compound: 2.9 mg/L (3.4 mg/1.16 L)
Pre-treatment of the toxicity control
- 5.8 mg of the test item were weighed and added to the test flasks, filled with ca. 300 mL of mineral medium
- 3.4 mL of the reference compound stock solution was added
- inoculum was added
- flasks volume was made up to 1.16 L with mineral medium
- the flasks were closed free from air bubbles with glass stoppers.
- initial concentration of test item: 5.0 mg/L (5.8 mg/1.16 L)
- initial concentration reference compound: 2.9 mg/L (3.4 mg/1.16 L)
Exposure conditions
- Test volume: 1.16 L
- Incubation time: 28 days
- Incubation temperature: 22 ± 2 °C - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Acros Organics, purity 99.9 %, batch no. A0357641
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 48
- Sampling time:
- 28 d
- Details on results:
- 15 % degradation after 7 d
36 % degradation after 14 d
51 % degradation after 21 d - Results with reference substance:
- The reference compound sodium benzoate showed 81 % degradation after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- oxygen depletion in blank control <= 1.5 mg dissolved O2/L after 28 d; reference compound ≥ 60 % for ready biodegradability in 14 d; toxicity control > 25 % degradation in 14 d
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Within 28 days, a degradation of 48 % was determined for the test substance. Thus, it is considered to be not readily biodegradable.
- Executive summary:
A study was performed to assess the ready biodegradability in accordance with the Council Regulation (EC) 440/2008 Method C.4-E “Closed Bottle Test” (2008). This test method is in all essential parts identical with OECD Guideline 301 D (adopted July 1992). The test substance was inoculated with secondary effluent to result in completely filled, closed flasks and incubated under aerobic conditions in the dark at 22 +- 2 °C for 28 days. During this period, the biodegradation was followed by analysis of dissolved oxygen. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, was expressed as a percentage of theoretical oxygen demand (ThOD). Flasks with reference control, inoculum blank and toxicity control were run in parallel.
A degradation of 48 % was determined after 28 d. Therefore, the test substance is considered to be “Not Readily Biodegradable“.
The reference compound sodium benzoate showed 81 % degradation after 14 days.
Reference
Description of key information
A study was performed to assess the ready biodegradability in accordance with the Council Regulation (EC) 440/2008 Method C.4-E “Closed Bottle Test” (2008), which is in all essential parts identical with OECD Guideline 301 D (adopted July 1992).The test substance was inoculated and incubated under aerobic conditions in the dark at 22 +- 2 °C for 28 days. During this period, the biodegradation was followed by analysis of dissolved oxygen. Flasks with reference control, inoculum blank and toxicity control were run in parallel.
A degradation of 48 % was determined after 28 d. Therefore, the test substance is considered to be “Not Readily Biodegradable“.
The reference compound sodium benzoate showed 81 % degradation after 14 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.