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EC number: 262-634-6 | CAS number: 61167-58-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Dec 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- Adopted 22 July 2010
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test vessels were prepared individually by weighing out the appropriate amount of the test item (0.0050, 0.0500, 0.5000, 0.5000, 0.5000, and 0.5000 g as active ingredient) and directly adding it to the respective vessels.
- Eluate: no
- Controls: yes, negative and abiotic control - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
New Bedford Waste Water Treatment Plant, New Bedford, Massachusetts, USA
- Preparation of inoculum for exposure: The inoculum received 1.75 mL of an undiluted synthetic sewage feed, was aerated overnight, and used for testing the next day. The inoculum was prepared one day before test initiation and had a final pH value of 7.23.
- Pretreatment: The sludge solution was passed through a 2-mm stainless steel sieve, concentrated by centrifuging for 10 minutes at 1000 rpm, and the resulting supernatant poured off. The sludge was washed with 600 mL of laboratory well water and centrifuged at 1000 rpm for 10 minutes, for a total of four times, with the supernatant being discarded each time. A small amount of the washed sludge was weighed and dried to calculate the quantity of wet sludge required to be suspended in 3.5 L of laboratory well water to obtain an activated sludge containing a solid level of three grams per liter (± 10%).
- Initial biomass concentration: 3 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.9 °C
- pH:
- 7.46 (abiotic control)
7.65 - 7.88 (remaining test vessels) - Nominal and measured concentrations:
- nominal: 10, 100, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glass beakers
- Aeration: yes
- No. of vessels per concentration (replicates): 1 (10 mg/L and 100 mg/L), 3 (1000 mg/L)
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
according to the guideline
EFFECT PARAMETERS MEASURED: Following approximately three hours of aeration, an aliquot of each sludge mixture was transferred to a 50 mL cylinder which was placed inside a 20 °C water bath placed over a stir plate. The pH of the sludge mixture was measured before the respiration measurement. The cylinders received a stir bar and were fitted with a dissolved oxygen probe after receiving the sample. The probes were attached to a Strathkelvin Instruments (SI) Model 928 oxygen system and a computer which ran the Strathkelvin software. Immediately after the dissolved oxygen probes were attached to the Strathkelvin system, the computer program was initiated so that oxygen depletion rates were measured starting at time zero. The oxygen depletion rate was terminated after a 10 min period. The sludge mixtures were stirred continuously while the oxygen measurements were taken. The temperature of the environmental chamber in which the test was conducted was monitored using a minimum/maximum thermometer. Solution pH was measured with a Yellow Springs Instrument (YSI) Model pH100 pH meter. The SI Multisample Activated Sludge Respirometer was used for determination of O2 consumption. The oxygen depletion rate was measured in a single sample from each beaker such that there was one replicate per beaker with zero headspace.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate:
At test termination, the respiration rate for the two control vessels was determined to be 51.8 and 46.9 mg O2/L/h, which met the acceptability criterion (i.e., within 30% of each other during the test).
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 8.3 mg/L
- Other: Respiration rate in the 3,5-dichlorophenol test vessels (1.0, 3.0, 10 and 30 mg/L) was 41.8, 38.5, 20.8, and 12.9 mg O2/L/hr, respectively. Compared to the controls, respiratory inhibition for the 3,5-dichlorophenol test vessels was 15.3, 22.0, 57.9, and 73.9%, respectively. - Reported statistics and error estimates:
- No statistical analysis was necessary since no effects were observed. The EC10 value (equivalent to a No-Observed-Effect-Concentration, NOEC) could not be mathematically determined due to an inverted dose curve.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Reference
Table 1: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
32.9 mg O2/g sludge dw/h |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
7 % |
yes |
Table 2: The pH, respiration rate, percent inhibition and EC50 values determined in all test vessels during the activated sludge respiration inhibition test.
Sample type |
pH |
Respiration rate (mg O2/L/h) |
% inhibition |
Abiotic |
7.46 |
- 0.2 |
NA |
Control 1 |
7.65 |
51.8 |
NA |
Control 2 |
7.72 |
46.9 |
NA |
3,5 dichlorophenol (mg/L) |
|||
1.0 |
7.73 |
41.8 |
15.3 |
3.0 |
7.80 |
38.5 |
22.0 |
10 |
7.85 |
20.8 |
57.9 |
30 |
7.88 |
12.9 |
73.9 |
EC50: 8.3 mg/L |
|
|
|
10 |
7.83 |
36.7 |
25.6 |
100 |
7.83 |
41.0 |
16.9 |
1000 |
7.84 |
42.4 |
14.1 |
1000 |
7.84 |
40.3 |
18.3 |
1000 |
7.84 |
38.9 |
21.2 |
EC50: > 1000 mg/L |
|
|
|
Description of key information
EC50 (3 h) > 1000 mg/L (nominal, activated sludge microorganisms, OECD 209)
Key value for chemical safety assessment
Additional information
One experimental study is available investigating the toxicity of 2-(1,1-dimethylethyl)-6-[[3-(1,1-dimethylethyl)-2-hydroxy-5-methylphenyl]methyl]-4-methylphenyl acrylate (CAS 61167-58-6) to activated sludge microorganisms. Activated sludge from a sewage treatment plant treating predominantly domestic sewage was used for the study. Three nominal concentrations of 10, 100 and 1000 mg/L were tested. The respiration rate was continuously measured for 10 min after 3 h exposure. Due to the inverted dose response curve no EC10 value could be statistically determined. However, the EC50 (3 h) was > 1000 mg/L (nominal).
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