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EC number: 229-158-0 | CAS number: 6420-33-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 October 2016 to 02 December 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK
- Storage conditions: washed sample is maintained in mineral medium (acc yo OECD 310) on continuous aeration at approximately 21 ºC
- Storage length:< 1 day
- Preparation of inoculum for exposure: in oculum at concentration of 30 mg SS/L
- Duration of test (contact time):
- 29 d
- Initial conc.:
- 10 other: mgC/L
- Based on:
- act. ingr.
- Initial conc.:
- 22.9 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD301
- Test temperature: 22-24 °C,
- pH: pre-treatment 7.7; after adjustment 7.5-7.6; on day 28 7.5-7.7
- Aeration of dilution water: CO2-free air
- Suspended solids concentration: 30 mgSS/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5 liter vessels
- Number of culture flasks/concentration: 2 vessels for controls, reference and test item, toxicity controls 1 velssel
- Method used to create aerobic conditions: bubbling of vessels with CO2-free air
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: NaOH in Dreschel bottle (2 traps)
SAMPLING
- Sampling frequency: first absorber on day 0, 2, 6, 8, 10, 14, 21, 28 and 29; second absorber on day 0 and 29
- Sample storage before analysis: either analysed directly or stored frozen
CONTROL AND BLANK SYSTEM
- Inoculum blank: in duplicate
- Abiotic sterile control: NA
- Toxicity control: single veseel
- Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- No indication of adsorption (filtered solutions 84-93% of nominal C-content)
- Test performance:
- positive control and toxicity control showed sufficient degradation to pass the validity criteria
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 21 d
- Remarks on result:
- other:
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9
- Sampling time:
- 29 d
- Remarks on result:
- other: after acidification with HCl
- Results with reference substance:
- the reference study attained 78% degradation over 14 days and 77% degradation at the end of the study
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable
- Executive summary:
In a CO2 evolution test the substance sludge was exposed to the substance over a 29 day period. No CO2 evolution was reported. Therefore the substance is considered not readily biodegradable.
Reference
Day |
% Biodegradation
|
||
|
Procedure Control |
Test Item |
Toxicity Control |
0 |
0 |
0 |
0 |
2 |
57 |
0 |
31 |
6 |
59 |
0 |
38 |
8 |
71 |
0 |
35 |
10 |
71 |
0 |
34 |
14 |
78 |
0 |
37 |
21 |
79 |
0 |
35 |
28 |
83 |
1 |
37 |
29 |
77 |
9 |
53 |
Description of key information
In a CO2 evolution test sludge was exposed to the substance over a 29 day period. No CO2 evolution was reported. Maximum degradation 9%. Therefore the substance is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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