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EC number: 210-043-9 | CAS number: 603-48-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: DIN 38 412 "Testverfahren mit Wasseroganismen (GruppeL). Allgemeine Hinweise zur Planung, Durchfuehrung und Auswertung Biologischer Test-Verfahren (L1)" und "Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische - Fischtest (L15)", June 1982
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N,N,N',N',N'',N''-hexamethyl-4,4',4''-methylidynetrianiline
- EC Number:
- 210-043-9
- EC Name:
- N,N,N',N',N'',N''-hexamethyl-4,4',4''-methylidynetrianiline
- Cas Number:
- 603-48-5
- Molecular formula:
- C25H31N3
- IUPAC Name:
- 4-{bis[4-(dimethylamino)phenyl]methyl}-N,N-dimethylaniline
- Test material form:
- solid
- Details on test material:
- - Purity: technical
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- The test substance was added to the test water without any pre-treatment.
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus idus L., Golden variety
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt, FRG
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 6.2 cm (5.7-6.9 cm)
- Weight at study initiation (mean and range, SD): 2.7 g (2.2-3.4 g)
- Method of breeding: not reported
- Maintenance of the brood fish: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Medical treatment: Twice with 0.05 mg/L malachite green chloride and once with 10 mg/L tetracycline hydrochloride
ACCLIMATION
- Acclimation period: ~ 6 weeks
- Acclimation conditions (same as test or not): Same
- Type and amount of food during acclimation: Growing feed F/B 50 (Ssniff Spezialdiaeten GMBH, D-4770 Soest, FRG)
- Feeding frequency during acclimation: ad libitum
- Health during acclimation (any mortality observed): No mortality was observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- ~ 2.5 mmol/L
- Test temperature:
- In the test substance vessel, 21°C at start of test, 21°C at 24 hours, 20°C at 48 hours, 21°C at 72 and 96 hours and In the control vessel, 21°C at start of test, 21°C at 24 hours, 20°C at 48 hours, 21°C at 72 and 96 hours
- pH:
- In the test substance vessel, 8.0 at start of test and at 24, 48, 72, and 96 hours and In the control vessel, 8.0 at start of test, 8.0 at 24 hours, 8.0 at 48 hours, and 7.9 at 72 and 96 hours
- Dissolved oxygen:
- In the test substance vessel, 7.4 mg/L at start of test, 8.1 mg/L at 24 hours, 8.3 mg/L at 48 hours, 8.2 mg/L at 72 hours, and 8.0 mg/L at 96 hours and In the control vessel, 7.8 mg/L at start of test, 6.7 mg/L at 24 hours, 7.4 mg/L at 48 hours, 7.2 mg/L at 72 hours, and 7.6 mg/L at 96 hours
- Conductivity:
- Max. 10 micro MHO
- Nominal and measured concentrations:
- 10000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass aquarium (30 cm x 22 cm x 24 cm)
- Material, size, headspace, fill volume: 10 litres
- Aeration: Aerated with oil-free air
- Type of flow-through (e.g. peristaltic or proportional diluter): Static
- Renewal rate of test solution (frequency/flow rate): No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 2.7
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11, Draft Sept. 1981 preparation from fully demineralized tap water
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No
- Intervals of water quality measurement: not reported - Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The test solution was cloudy and coloured. Undissolved test substance was visible on the water surface. No mortality or adverse symptoms were observed at any time point.
- Results with reference substance (positive control):
- Positive control of animals was conducted with chloroacetamide. LC50 after 48 hours: about 31 mg/L (determined Nov. 30, 1987)
- Reported statistics and error estimates:
- Calculation of the median lethal concentration (LC50) and, if possible, the LC5 and the LC95 were determined using probit analysis.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Fish mortality after 1, 4, 24, 48, 72, and 96 hours of exposure
Concentration
Number of fish
Exposure time
Mortality
% Mortality
0
10
1
4
24
48
72
96
0
0
0
0
0
0
0
0
0
0
0
0
10000 mg/L
10
1
4
24
48
72
96
0
0
0
0
0
0
0
0
0
0
0
0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hour LC50 was greater than 10000 mg/L, the highest concentration tested.
- Executive summary:
Groups of 10 golden orfe fish, Leuciscus idus L., were exposed for 96 hours to 0 or 10000 mg/L of the test substance dissolved in water. There was no mortality and no signs of toxicity.
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